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HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells

BACKGROUND: Heat-shock protein 70 (HSP70) is a member of the heat-shock protein family which is expressed in various types of cancer and associated with apoptosis in cancer cells. However, the role of HSP70 in the regulation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (...

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Autores principales: Zhang, Kai, Zhai, Ruonan, Xue, Teng, Xu, Xiaoyan, Ren, Yanan, Ma, Mingze, Shi, Fengxian, Wang, Hang, Wang, Na, Zhou, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798149/
https://www.ncbi.nlm.nih.gov/pubmed/35117461
http://dx.doi.org/10.21037/tcr.2019.12.100
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author Zhang, Kai
Zhai, Ruonan
Xue, Teng
Xu, Xiaoyan
Ren, Yanan
Ma, Mingze
Shi, Fengxian
Wang, Hang
Wang, Na
Zhou, Fang
author_facet Zhang, Kai
Zhai, Ruonan
Xue, Teng
Xu, Xiaoyan
Ren, Yanan
Ma, Mingze
Shi, Fengxian
Wang, Hang
Wang, Na
Zhou, Fang
author_sort Zhang, Kai
collection PubMed
description BACKGROUND: Heat-shock protein 70 (HSP70) is a member of the heat-shock protein family which is expressed in various types of cancer and associated with apoptosis in cancer cells. However, the role of HSP70 in the regulation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK)-dependent growth and apoptosis in lung cancer cells remains largely unknown. METHODS: In this study, we conducted in vitro experiments. First, we examined the effect of HSP70 by treatment with mild heat and JNK/p38 MAPK inhibitors on cell proliferation in A549 cells by MTT assay. And then, through the use of flow cytometric assay, we examined the effect of HSP70 by treatment with mild heat and JNK/p38 MAPK inhibitors on cell apoptosis in A549 cells. Finally, we determined the role of HSP70 in the regulation of JNK and p38 MAPK-dependent growth and apoptosis in A549 cells by siRNA HSPAIA-2009 and Western blot. RESULTS: We found that treatment of the cells with mild heat and JNK/p38 MAPK pathway inhibitors (SP600125 and SB203580) promoted cell proliferation in the presence of actinomycin D (ActD) in A549 cells. We also showed that treatment with mild heat or SP600125 and SB203580 significantly slowed the steps of apoptosis induced by ActD in A549 cells. Moreover, we found that HSP70 overexpression induced by mild heat markedly decreased the expression of cell growth and apoptosis-related protein p-JNK, p38 and caspase-3. By contrast, knockdown of HSP70 by siRNA HSPAIA-2009 effectively promoted the expression of the JNK and p38 MAPK. CONCLUSIONS: Our results indicate that HSP70 plays an important role in lung cancer growth and apoptosis through the activation of JNK and p38 MAPK signaling in actinomycin-D-treated lung cancer A549 cells.
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spelling pubmed-87981492022-02-02 HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells Zhang, Kai Zhai, Ruonan Xue, Teng Xu, Xiaoyan Ren, Yanan Ma, Mingze Shi, Fengxian Wang, Hang Wang, Na Zhou, Fang Transl Cancer Res Original Article BACKGROUND: Heat-shock protein 70 (HSP70) is a member of the heat-shock protein family which is expressed in various types of cancer and associated with apoptosis in cancer cells. However, the role of HSP70 in the regulation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK)-dependent growth and apoptosis in lung cancer cells remains largely unknown. METHODS: In this study, we conducted in vitro experiments. First, we examined the effect of HSP70 by treatment with mild heat and JNK/p38 MAPK inhibitors on cell proliferation in A549 cells by MTT assay. And then, through the use of flow cytometric assay, we examined the effect of HSP70 by treatment with mild heat and JNK/p38 MAPK inhibitors on cell apoptosis in A549 cells. Finally, we determined the role of HSP70 in the regulation of JNK and p38 MAPK-dependent growth and apoptosis in A549 cells by siRNA HSPAIA-2009 and Western blot. RESULTS: We found that treatment of the cells with mild heat and JNK/p38 MAPK pathway inhibitors (SP600125 and SB203580) promoted cell proliferation in the presence of actinomycin D (ActD) in A549 cells. We also showed that treatment with mild heat or SP600125 and SB203580 significantly slowed the steps of apoptosis induced by ActD in A549 cells. Moreover, we found that HSP70 overexpression induced by mild heat markedly decreased the expression of cell growth and apoptosis-related protein p-JNK, p38 and caspase-3. By contrast, knockdown of HSP70 by siRNA HSPAIA-2009 effectively promoted the expression of the JNK and p38 MAPK. CONCLUSIONS: Our results indicate that HSP70 plays an important role in lung cancer growth and apoptosis through the activation of JNK and p38 MAPK signaling in actinomycin-D-treated lung cancer A549 cells. AME Publishing Company 2020-02 /pmc/articles/PMC8798149/ /pubmed/35117461 http://dx.doi.org/10.21037/tcr.2019.12.100 Text en 2020 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
spellingShingle Original Article
Zhang, Kai
Zhai, Ruonan
Xue, Teng
Xu, Xiaoyan
Ren, Yanan
Ma, Mingze
Shi, Fengxian
Wang, Hang
Wang, Na
Zhou, Fang
HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells
title HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells
title_full HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells
title_fullStr HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells
title_full_unstemmed HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells
title_short HSP70 regulates cell proliferation and apoptosis in actinomycin-D-treated lung cancer cells
title_sort hsp70 regulates cell proliferation and apoptosis in actinomycin-d-treated lung cancer cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798149/
https://www.ncbi.nlm.nih.gov/pubmed/35117461
http://dx.doi.org/10.21037/tcr.2019.12.100
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