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miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues
BACKGROUND: miR-138 was thought to be anti-tumor miRNA for its ability of suppressing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-138 and its combination with miR-21 in formalin-fixed paraffin-embedded (FFPE) tissues of papillary thyroid cancers (P...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798326/ https://www.ncbi.nlm.nih.gov/pubmed/35116921 http://dx.doi.org/10.21037/tcr.2019.08.14 |
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author | Wang, Li Duan, Yuanyuan Peng, Wen Qu, Caixing Lin, Jiang Deng, Zhaoqun You, Chao Wu, Chenguang |
author_facet | Wang, Li Duan, Yuanyuan Peng, Wen Qu, Caixing Lin, Jiang Deng, Zhaoqun You, Chao Wu, Chenguang |
author_sort | Wang, Li |
collection | PubMed |
description | BACKGROUND: miR-138 was thought to be anti-tumor miRNA for its ability of suppressing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-138 and its combination with miR-21 in formalin-fixed paraffin-embedded (FFPE) tissues of papillary thyroid cancers (PTCs). METHODS: miR-138/miR-21 expression was tested in 101 PTC and 51 benign thyroid nodule (control) patients using quantitative real-time PCR. Clinical and pathological characteristics of enrolled subjects were obtained from medical records. RESULTS: miR-138 was significantly down-regulated in PTC compared with controls (median 0.0122 vs. 0.0943), and miR-138 exhibited capability of discriminating PTC from controls, with a diagnostic accuracy of 71.1% (sensitivity 84.3%, specificity 49.5%). A combination of miR-138 and miR-21 increased the diagnostic accuracy to 79.1% (sensitivity 73.3%, specificity 76.5%). Multivariate logistic regression confirmed the association between miR-138/miR-21 expression and PTC diagnosis. Moreover, miR-138 expression was further down-regulated in PTC with capsule invasion compared with PTC tissues without capsule invasion (median 0.0043 vs. 0.0373, P=0.003). CONCLUSIONS: MiR-138 expression was not only associated with onset of PTC, but also the aggressiveness of PTC. A combination of miR-138 and miR-21 enhanced the diagnostic value of individual biomarkers. |
format | Online Article Text |
id | pubmed-8798326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-87983262022-02-02 miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues Wang, Li Duan, Yuanyuan Peng, Wen Qu, Caixing Lin, Jiang Deng, Zhaoqun You, Chao Wu, Chenguang Transl Cancer Res Original Article BACKGROUND: miR-138 was thought to be anti-tumor miRNA for its ability of suppressing tumor growth. The objective of this study was to evaluate the potential predictive value of miR-138 and its combination with miR-21 in formalin-fixed paraffin-embedded (FFPE) tissues of papillary thyroid cancers (PTCs). METHODS: miR-138/miR-21 expression was tested in 101 PTC and 51 benign thyroid nodule (control) patients using quantitative real-time PCR. Clinical and pathological characteristics of enrolled subjects were obtained from medical records. RESULTS: miR-138 was significantly down-regulated in PTC compared with controls (median 0.0122 vs. 0.0943), and miR-138 exhibited capability of discriminating PTC from controls, with a diagnostic accuracy of 71.1% (sensitivity 84.3%, specificity 49.5%). A combination of miR-138 and miR-21 increased the diagnostic accuracy to 79.1% (sensitivity 73.3%, specificity 76.5%). Multivariate logistic regression confirmed the association between miR-138/miR-21 expression and PTC diagnosis. Moreover, miR-138 expression was further down-regulated in PTC with capsule invasion compared with PTC tissues without capsule invasion (median 0.0043 vs. 0.0373, P=0.003). CONCLUSIONS: MiR-138 expression was not only associated with onset of PTC, but also the aggressiveness of PTC. A combination of miR-138 and miR-21 enhanced the diagnostic value of individual biomarkers. AME Publishing Company 2019-09 /pmc/articles/PMC8798326/ /pubmed/35116921 http://dx.doi.org/10.21037/tcr.2019.08.14 Text en 2019 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/. |
spellingShingle | Original Article Wang, Li Duan, Yuanyuan Peng, Wen Qu, Caixing Lin, Jiang Deng, Zhaoqun You, Chao Wu, Chenguang miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
title | miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
title_full | miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
title_fullStr | miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
title_full_unstemmed | miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
title_short | miR-21 facilitates the diagnostic value of miR-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
title_sort | mir-21 facilitates the diagnostic value of mir-138 for papillary thyroid cancer in formalin-fixed paraffin-embedded tissues |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798326/ https://www.ncbi.nlm.nih.gov/pubmed/35116921 http://dx.doi.org/10.21037/tcr.2019.08.14 |
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