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Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma

BACKGROUND: The role of Ral and RalGAPs on the progression of head and neck squamous cell carcinoma (HNSC) remains unclear. METHODS: The predesigned siRNAs against RalGAPs were transfected into cells to evaluate the effect on RalA activation. The Data from TCGA and GTEx were combined to analyze the...

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Autores principales: Liu, Shan, Shi, Congyu, Wang, Xiaoyi, Ma, Xiangrui, Gao, Pan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799020/
https://www.ncbi.nlm.nih.gov/pubmed/35116360
http://dx.doi.org/10.21037/tcr-21-1489
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author Liu, Shan
Shi, Congyu
Wang, Xiaoyi
Ma, Xiangrui
Gao, Pan
author_facet Liu, Shan
Shi, Congyu
Wang, Xiaoyi
Ma, Xiangrui
Gao, Pan
author_sort Liu, Shan
collection PubMed
description BACKGROUND: The role of Ral and RalGAPs on the progression of head and neck squamous cell carcinoma (HNSC) remains unclear. METHODS: The predesigned siRNAs against RalGAPs were transfected into cells to evaluate the effect on RalA activation. The Data from TCGA and GTEx were combined to analyze the pan-cancer gene expression of RalA and RalGAPs in cancer and adjacent normal tissues. Kaplan-Meier analysis was used to assess the predictive value of RalA and RalGAPs expression on the overall survival of patients with HNSC. Methylation-specific PCR in vitro and next-generation bisulfite sequencing in vivo were used to evaluate the association between DNA methylation and the down-regulation of RalGAPs. RESULTS: RalGAPs negatively regulated RalA activation. HNSC patients with low level of RalGAPα2 had worse overall survival. The promoter of RalGAPα2 was widely methylated in comparison to RalGAPα1 and the DNA methylation level of RalGAPα2 promoter was increased in HNSC tissues and associated with the presence of neck lymph node metastasis. CONCLUSIONS: RalA and RalGAPs could act as a specific predictor to assess the prognosis of HNSC. DNA methylation might be a potential mechanism that downregulated the RalGAPα2 expression.
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spelling pubmed-87990202022-02-02 Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma Liu, Shan Shi, Congyu Wang, Xiaoyi Ma, Xiangrui Gao, Pan Transl Cancer Res Original Article BACKGROUND: The role of Ral and RalGAPs on the progression of head and neck squamous cell carcinoma (HNSC) remains unclear. METHODS: The predesigned siRNAs against RalGAPs were transfected into cells to evaluate the effect on RalA activation. The Data from TCGA and GTEx were combined to analyze the pan-cancer gene expression of RalA and RalGAPs in cancer and adjacent normal tissues. Kaplan-Meier analysis was used to assess the predictive value of RalA and RalGAPs expression on the overall survival of patients with HNSC. Methylation-specific PCR in vitro and next-generation bisulfite sequencing in vivo were used to evaluate the association between DNA methylation and the down-regulation of RalGAPs. RESULTS: RalGAPs negatively regulated RalA activation. HNSC patients with low level of RalGAPα2 had worse overall survival. The promoter of RalGAPα2 was widely methylated in comparison to RalGAPα1 and the DNA methylation level of RalGAPα2 promoter was increased in HNSC tissues and associated with the presence of neck lymph node metastasis. CONCLUSIONS: RalA and RalGAPs could act as a specific predictor to assess the prognosis of HNSC. DNA methylation might be a potential mechanism that downregulated the RalGAPα2 expression. AME Publishing Company 2021-12 /pmc/articles/PMC8799020/ /pubmed/35116360 http://dx.doi.org/10.21037/tcr-21-1489 Text en 2021 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
spellingShingle Original Article
Liu, Shan
Shi, Congyu
Wang, Xiaoyi
Ma, Xiangrui
Gao, Pan
Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma
title Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma
title_full Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma
title_fullStr Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma
title_full_unstemmed Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma
title_short Low expression of RalGAPs associates with the poorer overall survival of head and neck squamous cell carcinoma
title_sort low expression of ralgaps associates with the poorer overall survival of head and neck squamous cell carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799020/
https://www.ncbi.nlm.nih.gov/pubmed/35116360
http://dx.doi.org/10.21037/tcr-21-1489
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