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MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma

BACKGROUND: MicroRNAs are found to be aberrantly expressed in multiple cancers, including glioblastoma (GBM), and microRNA-221 (miR-221) has been verified as an oncogene in various human cancers. Nevertheless, the role of miR-221 in GBM is unclear. This study aimed to investigate the miR-221 express...

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Autores principales: Chang, Liang, Yin, Lisheng, Zhang, Dongzhi, Wang, Chao, Li, Guofu, Tan, Chunlei, Zhang, Xuexin, Su, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799047/
https://www.ncbi.nlm.nih.gov/pubmed/35116434
http://dx.doi.org/10.21037/tcr-21-99
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author Chang, Liang
Yin, Lisheng
Zhang, Dongzhi
Wang, Chao
Li, Guofu
Tan, Chunlei
Zhang, Xuexin
Su, Jun
author_facet Chang, Liang
Yin, Lisheng
Zhang, Dongzhi
Wang, Chao
Li, Guofu
Tan, Chunlei
Zhang, Xuexin
Su, Jun
author_sort Chang, Liang
collection PubMed
description BACKGROUND: MicroRNAs are found to be aberrantly expressed in multiple cancers, including glioblastoma (GBM), and microRNA-221 (miR-221) has been verified as an oncogene in various human cancers. Nevertheless, the role of miR-221 in GBM is unclear. This study aimed to investigate the miR-221 expression level in GBM and to evaluate its function and underlying mechanisms. METHODS: Western blotting and qPCR were used to determine the expression of human hedgehog-interacting protein (HHIP) and miR-221 levels. MiR-221-inhibited cell models were constructed, and siRNA was used for HHIP silencing. Cell proliferation was analyzed by MTT and colony formation assays and a subcutaneous xenograft model. Cell migration and invasion was analyzed by wound healing and Transwell invasion assays. A dual luciferase reporter assay system was used to clarify the relationship between miR-221 and HHIP. RESULTS: The results of this study revealed that miR-221 expression was upregulated in GBM tissues and A172, U251, as well as T98G cells, as detected by real-time PCR analysis. MTT, Transwell, and colony formation assays revealed that miR-221 knockdown could suppress GBM cells from proliferating, migrating, and invading in vitro. Moreover, animal experiments showed that tumor growth in vivo was inhibited when miR-221 expression decreased. Furthermore, HHIP was predicted and verified to be a target of miR-221 by bioinformatics analysis, and luciferase and western blot assays. In addition, HHIP silencing rescued the suppressive effect of a miR-221 inhibitor on the proliferation, migration, and invasion of GBM cells. CONCLUSIONS: Our results indicated that miR-221 is upregulated in GBM and enhances tumor progression by targeting HHIP, which suggests this may be a potential therapeutic target for GBM.
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spelling pubmed-87990472022-02-02 MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma Chang, Liang Yin, Lisheng Zhang, Dongzhi Wang, Chao Li, Guofu Tan, Chunlei Zhang, Xuexin Su, Jun Transl Cancer Res Original Article BACKGROUND: MicroRNAs are found to be aberrantly expressed in multiple cancers, including glioblastoma (GBM), and microRNA-221 (miR-221) has been verified as an oncogene in various human cancers. Nevertheless, the role of miR-221 in GBM is unclear. This study aimed to investigate the miR-221 expression level in GBM and to evaluate its function and underlying mechanisms. METHODS: Western blotting and qPCR were used to determine the expression of human hedgehog-interacting protein (HHIP) and miR-221 levels. MiR-221-inhibited cell models were constructed, and siRNA was used for HHIP silencing. Cell proliferation was analyzed by MTT and colony formation assays and a subcutaneous xenograft model. Cell migration and invasion was analyzed by wound healing and Transwell invasion assays. A dual luciferase reporter assay system was used to clarify the relationship between miR-221 and HHIP. RESULTS: The results of this study revealed that miR-221 expression was upregulated in GBM tissues and A172, U251, as well as T98G cells, as detected by real-time PCR analysis. MTT, Transwell, and colony formation assays revealed that miR-221 knockdown could suppress GBM cells from proliferating, migrating, and invading in vitro. Moreover, animal experiments showed that tumor growth in vivo was inhibited when miR-221 expression decreased. Furthermore, HHIP was predicted and verified to be a target of miR-221 by bioinformatics analysis, and luciferase and western blot assays. In addition, HHIP silencing rescued the suppressive effect of a miR-221 inhibitor on the proliferation, migration, and invasion of GBM cells. CONCLUSIONS: Our results indicated that miR-221 is upregulated in GBM and enhances tumor progression by targeting HHIP, which suggests this may be a potential therapeutic target for GBM. AME Publishing Company 2021-02 /pmc/articles/PMC8799047/ /pubmed/35116434 http://dx.doi.org/10.21037/tcr-21-99 Text en 2021 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
spellingShingle Original Article
Chang, Liang
Yin, Lisheng
Zhang, Dongzhi
Wang, Chao
Li, Guofu
Tan, Chunlei
Zhang, Xuexin
Su, Jun
MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma
title MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma
title_full MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma
title_fullStr MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma
title_full_unstemmed MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma
title_short MicroRNA-221 promotes tumor progression by targeting HHIP in human glioblastoma
title_sort microrna-221 promotes tumor progression by targeting hhip in human glioblastoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799047/
https://www.ncbi.nlm.nih.gov/pubmed/35116434
http://dx.doi.org/10.21037/tcr-21-99
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