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The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia

BACKGROUND: The present research focuses on preeclampsia (PE), a clinically relevant pregnancy disease. To date, the majority of research on PE was centered on placental insufficiency. However, the genes that regulate these processes, and the exact molecular mechanisms modulating these processes, ar...

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Autores principales: Hou, Fei, Jin, Hua, Cao, Luquan, Jiao, Xinlin, Wang, Bingyu, Liu, Haiying, Cui, Baoxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799331/
https://www.ncbi.nlm.nih.gov/pubmed/35096127
http://dx.doi.org/10.1155/2022/1457398
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author Hou, Fei
Jin, Hua
Cao, Luquan
Jiao, Xinlin
Wang, Bingyu
Liu, Haiying
Cui, Baoxia
author_facet Hou, Fei
Jin, Hua
Cao, Luquan
Jiao, Xinlin
Wang, Bingyu
Liu, Haiying
Cui, Baoxia
author_sort Hou, Fei
collection PubMed
description BACKGROUND: The present research focuses on preeclampsia (PE), a clinically relevant pregnancy disease. To date, the majority of research on PE was centered on placental insufficiency. However, the genes that regulate these processes, and the exact molecular mechanisms modulating these processes, are still unclear. METHODS: We obtained placentae from a clinically well-specified group of patients with preeclampsia and gestationally matched control pregnancies in order to evaluate the expression of homeobox gene DLX3 by immunohistochemical staining, real-time PCR, and Western immunoblotting and determine the function of DLX3 utilizing lentivirus transfection in HTR-8/SVneo cells. RESULTS: In the present study, we detected DLX3 expression in a clinically well defined cohort of preeclampsia-affected and gestation-matched control pregnancies. As opposed to the controls, DLX3 was overexpressed in preeclampsia-affected placentae. Moreover, we found that the in vitro cell growth and invasive ability of HTR8/SVneo cells was enhanced by the exogenous overexpression of DLX3 (P < 0.05). It can be seen that DLX3 influences the cell cycle of HTR-8/SVneo cells in vitro. CONCLUSIONS: DLX3 has been shown to be strongly related to normal placental growth as well as the pathophysiology of preeclampsia. The imbalanced expression of DLX3 may perform an integral function in the occurrence and progression of preeclampsia.
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spelling pubmed-87993312022-01-29 The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia Hou, Fei Jin, Hua Cao, Luquan Jiao, Xinlin Wang, Bingyu Liu, Haiying Cui, Baoxia Comput Math Methods Med Research Article BACKGROUND: The present research focuses on preeclampsia (PE), a clinically relevant pregnancy disease. To date, the majority of research on PE was centered on placental insufficiency. However, the genes that regulate these processes, and the exact molecular mechanisms modulating these processes, are still unclear. METHODS: We obtained placentae from a clinically well-specified group of patients with preeclampsia and gestationally matched control pregnancies in order to evaluate the expression of homeobox gene DLX3 by immunohistochemical staining, real-time PCR, and Western immunoblotting and determine the function of DLX3 utilizing lentivirus transfection in HTR-8/SVneo cells. RESULTS: In the present study, we detected DLX3 expression in a clinically well defined cohort of preeclampsia-affected and gestation-matched control pregnancies. As opposed to the controls, DLX3 was overexpressed in preeclampsia-affected placentae. Moreover, we found that the in vitro cell growth and invasive ability of HTR8/SVneo cells was enhanced by the exogenous overexpression of DLX3 (P < 0.05). It can be seen that DLX3 influences the cell cycle of HTR-8/SVneo cells in vitro. CONCLUSIONS: DLX3 has been shown to be strongly related to normal placental growth as well as the pathophysiology of preeclampsia. The imbalanced expression of DLX3 may perform an integral function in the occurrence and progression of preeclampsia. Hindawi 2022-01-21 /pmc/articles/PMC8799331/ /pubmed/35096127 http://dx.doi.org/10.1155/2022/1457398 Text en Copyright © 2022 Fei Hou et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hou, Fei
Jin, Hua
Cao, Luquan
Jiao, Xinlin
Wang, Bingyu
Liu, Haiying
Cui, Baoxia
The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia
title The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia
title_full The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia
title_fullStr The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia
title_full_unstemmed The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia
title_short The Imbalance Expression of DLX3 May Perform Critical Function in the Occurrence and Progression of Preeclampsia
title_sort imbalance expression of dlx3 may perform critical function in the occurrence and progression of preeclampsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799331/
https://www.ncbi.nlm.nih.gov/pubmed/35096127
http://dx.doi.org/10.1155/2022/1457398
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