Development of a sensitive, quantitative assay with broad subtype specificity for detection of total HIV-1 nucleic acids in plasma and PBMC

An LTR-based quantitative PCR (qPCR) assay was modified and optimized for the quantification of total HIV-1 nucleic acids in plasma and PBMC. TaqMan qPCR primers and probes were designed against the NCBI/LANL HIV-1 compendium database by analyzing sequences used in assays for sensitive cross-clade d...

Descripción completa

Detalles Bibliográficos
Autores principales: Kibirige, C. N., Manak, M., King, D., Abel, B., Hack, H., Wooding, D., Liu, Y., Fernandez, N., Dalel, J., Kaye, Steve, Imami, N., Jagodzinski, L., Gilmour, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799642/
https://www.ncbi.nlm.nih.gov/pubmed/35091568
http://dx.doi.org/10.1038/s41598-021-03016-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799642/
https://www.ncbi.nlm.nih.gov/pubmed/35091568
http://dx.doi.org/10.1038/s41598-021-03016-1

Ejemplares similares