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Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs
Carbapenems are broad-spectrum antibiotics widely used for the treatment of human infections caused by multidrug-resistant (MDR) Gram-negative bacteria. However, emerging carbapenemase-producing Enterobacterales (CPE) are rising as a public threat to human and animal health. We screened clinical bac...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799648/ https://www.ncbi.nlm.nih.gov/pubmed/35091689 http://dx.doi.org/10.1038/s41598-022-05585-1 |
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author | Kyung, Su Min Choi, Sung-Woon Lim, Jaewon Shim, Soojin Kim, Suji Im, Young Bin Lee, Na-Eun Hwang, Cheol-Yong Kim, Donghyuk Yoo, Han Sang |
author_facet | Kyung, Su Min Choi, Sung-Woon Lim, Jaewon Shim, Soojin Kim, Suji Im, Young Bin Lee, Na-Eun Hwang, Cheol-Yong Kim, Donghyuk Yoo, Han Sang |
author_sort | Kyung, Su Min |
collection | PubMed |
description | Carbapenems are broad-spectrum antibiotics widely used for the treatment of human infections caused by multidrug-resistant (MDR) Gram-negative bacteria. However, emerging carbapenemase-producing Enterobacterales (CPE) are rising as a public threat to human and animal health. We screened clinical bacterial isolates from 241 dogs and 18 cats hospitalized at Veterinary Medical Teaching Hospital, Seoul National University, from 2018 to 2020 for carbapenemase production. In our study, 5 strains of metallo-β-lactamase NDM-5-producing Escherichia coli and Klebsiella pneumoniae were isolated from 4 different dogs. Multilocus sequence typing (MLST) results showed that all E. coli strains were ST410 and all K. pneumoniae strains were ST378. Whole genome analysis of the plasmid showed that bla(NDM-5) is carried on a IncX3 plasmid, showing a high concordance rate with plasmids detected worldwide in human and animal isolates. The bla(NDM) gene was associated with the ble(MBL) gene and the ISAba125 element, truncated with the IS5 element. The results of this study show that CPE has already become as a threat to both animals and humans in our society, posing the necessity to solve it in terms of "One Health". Therefore, preventive strategies should be developed to prevent the spread of CPE in animal and human societies. |
format | Online Article Text |
id | pubmed-8799648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-87996482022-02-01 Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs Kyung, Su Min Choi, Sung-Woon Lim, Jaewon Shim, Soojin Kim, Suji Im, Young Bin Lee, Na-Eun Hwang, Cheol-Yong Kim, Donghyuk Yoo, Han Sang Sci Rep Article Carbapenems are broad-spectrum antibiotics widely used for the treatment of human infections caused by multidrug-resistant (MDR) Gram-negative bacteria. However, emerging carbapenemase-producing Enterobacterales (CPE) are rising as a public threat to human and animal health. We screened clinical bacterial isolates from 241 dogs and 18 cats hospitalized at Veterinary Medical Teaching Hospital, Seoul National University, from 2018 to 2020 for carbapenemase production. In our study, 5 strains of metallo-β-lactamase NDM-5-producing Escherichia coli and Klebsiella pneumoniae were isolated from 4 different dogs. Multilocus sequence typing (MLST) results showed that all E. coli strains were ST410 and all K. pneumoniae strains were ST378. Whole genome analysis of the plasmid showed that bla(NDM-5) is carried on a IncX3 plasmid, showing a high concordance rate with plasmids detected worldwide in human and animal isolates. The bla(NDM) gene was associated with the ble(MBL) gene and the ISAba125 element, truncated with the IS5 element. The results of this study show that CPE has already become as a threat to both animals and humans in our society, posing the necessity to solve it in terms of "One Health". Therefore, preventive strategies should be developed to prevent the spread of CPE in animal and human societies. Nature Publishing Group UK 2022-01-28 /pmc/articles/PMC8799648/ /pubmed/35091689 http://dx.doi.org/10.1038/s41598-022-05585-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kyung, Su Min Choi, Sung-Woon Lim, Jaewon Shim, Soojin Kim, Suji Im, Young Bin Lee, Na-Eun Hwang, Cheol-Yong Kim, Donghyuk Yoo, Han Sang Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs |
title | Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs |
title_full | Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs |
title_fullStr | Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs |
title_full_unstemmed | Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs |
title_short | Comparative genomic analysis of plasmids encoding metallo-β-lactamase NDM-5 in Enterobacterales Korean isolates from companion dogs |
title_sort | comparative genomic analysis of plasmids encoding metallo-β-lactamase ndm-5 in enterobacterales korean isolates from companion dogs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799648/ https://www.ncbi.nlm.nih.gov/pubmed/35091689 http://dx.doi.org/10.1038/s41598-022-05585-1 |
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