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Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major)
Globally increasing levels of artificial light at night (ALAN) are associated with shifting rhythms of behaviour in many wild species. However, it is unclear whether changes in behavioural timing are paralleled by consistent shifts in the molecular clock and its associated physiological pathways. In...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799718/ https://www.ncbi.nlm.nih.gov/pubmed/35091579 http://dx.doi.org/10.1038/s41598-022-05059-4 |
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author | Dominoni, Davide M. de Jong, Maaike van Oers, Kees O’Shaughnessy, Peter Blackburn, Gavin J. Atema, Els Mateman, A. Christa D’Amelio, Pietro B. Trost, Lisa Bellingham, Michelle Clark, Jessica Visser, Marcel E. Helm, Barbara |
author_facet | Dominoni, Davide M. de Jong, Maaike van Oers, Kees O’Shaughnessy, Peter Blackburn, Gavin J. Atema, Els Mateman, A. Christa D’Amelio, Pietro B. Trost, Lisa Bellingham, Michelle Clark, Jessica Visser, Marcel E. Helm, Barbara |
author_sort | Dominoni, Davide M. |
collection | PubMed |
description | Globally increasing levels of artificial light at night (ALAN) are associated with shifting rhythms of behaviour in many wild species. However, it is unclear whether changes in behavioural timing are paralleled by consistent shifts in the molecular clock and its associated physiological pathways. Inconsistent shifts between behavioural and molecular rhythms, and between different tissues and physiological systems, disrupt the circadian system, which coordinates all major body functions. We therefore compared behavioural, transcriptional and metabolomic responses of captive great tits (Parus major) to three ALAN intensities or to dark nights, recording activity and sampling brain, liver, spleen and blood at mid-day and midnight. ALAN advanced wake-up time, and this shift was paralleled by advanced expression of the clock gene BMAL1 in all tissues, suggesting close links between behaviour and clock gene expression across tissues. However, further analysis of gene expression and metabolites revealed that clock shifts were inconsistent across physiological systems. Untargeted metabolomic profiling showed that only 9.7% of the 755 analysed metabolites followed the behavioural shift. This high level of desynchronization indicates that ALAN disrupted the circadian system on a deep, easily overlooked level. Thus, circadian disruption could be a key mediator of health impacts of ALAN on wild animals. |
format | Online Article Text |
id | pubmed-8799718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-87997182022-02-01 Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) Dominoni, Davide M. de Jong, Maaike van Oers, Kees O’Shaughnessy, Peter Blackburn, Gavin J. Atema, Els Mateman, A. Christa D’Amelio, Pietro B. Trost, Lisa Bellingham, Michelle Clark, Jessica Visser, Marcel E. Helm, Barbara Sci Rep Article Globally increasing levels of artificial light at night (ALAN) are associated with shifting rhythms of behaviour in many wild species. However, it is unclear whether changes in behavioural timing are paralleled by consistent shifts in the molecular clock and its associated physiological pathways. Inconsistent shifts between behavioural and molecular rhythms, and between different tissues and physiological systems, disrupt the circadian system, which coordinates all major body functions. We therefore compared behavioural, transcriptional and metabolomic responses of captive great tits (Parus major) to three ALAN intensities or to dark nights, recording activity and sampling brain, liver, spleen and blood at mid-day and midnight. ALAN advanced wake-up time, and this shift was paralleled by advanced expression of the clock gene BMAL1 in all tissues, suggesting close links between behaviour and clock gene expression across tissues. However, further analysis of gene expression and metabolites revealed that clock shifts were inconsistent across physiological systems. Untargeted metabolomic profiling showed that only 9.7% of the 755 analysed metabolites followed the behavioural shift. This high level of desynchronization indicates that ALAN disrupted the circadian system on a deep, easily overlooked level. Thus, circadian disruption could be a key mediator of health impacts of ALAN on wild animals. Nature Publishing Group UK 2022-01-28 /pmc/articles/PMC8799718/ /pubmed/35091579 http://dx.doi.org/10.1038/s41598-022-05059-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Dominoni, Davide M. de Jong, Maaike van Oers, Kees O’Shaughnessy, Peter Blackburn, Gavin J. Atema, Els Mateman, A. Christa D’Amelio, Pietro B. Trost, Lisa Bellingham, Michelle Clark, Jessica Visser, Marcel E. Helm, Barbara Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) |
title | Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) |
title_full | Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) |
title_fullStr | Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) |
title_full_unstemmed | Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) |
title_short | Integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male Great tits (Parus major) |
title_sort | integrated molecular and behavioural data reveal deep circadian disruption in response to artificial light at night in male great tits (parus major) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799718/ https://www.ncbi.nlm.nih.gov/pubmed/35091579 http://dx.doi.org/10.1038/s41598-022-05059-4 |
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