Cargando…
Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis
Radiotherapy (RT) is an effective cancer treatment modality, but standard RT often causes collateral damage to nearby healthy tissues. To increase therapeutic ratio, radiosensitization via gold nanoparticles (GNPs) has been shown to be effective. One challenge is that megavoltage beams generated by...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799734/ https://www.ncbi.nlm.nih.gov/pubmed/35091583 http://dx.doi.org/10.1038/s41598-022-05339-z |
_version_ | 1784642125385695232 |
---|---|
author | Piccolo, Olivia Lincoln, John D. Melong, Nicole Orr, Benno C. Fernandez, Nicholas R. Borsavage, Jennifer Berman, Jason N. Robar, James Ha, Michael N. |
author_facet | Piccolo, Olivia Lincoln, John D. Melong, Nicole Orr, Benno C. Fernandez, Nicholas R. Borsavage, Jennifer Berman, Jason N. Robar, James Ha, Michael N. |
author_sort | Piccolo, Olivia |
collection | PubMed |
description | Radiotherapy (RT) is an effective cancer treatment modality, but standard RT often causes collateral damage to nearby healthy tissues. To increase therapeutic ratio, radiosensitization via gold nanoparticles (GNPs) has been shown to be effective. One challenge is that megavoltage beams generated by clinical linear accelerators are poor initiators of the photoelectric effect. Previous computer models predicted that a diamond target beam (DTB) will yield 400% more low-energy photons, increasing the probability of interacting with GNPs to enhance the radiation dose by 7.7-fold in the GNP vicinity. After testing DTB radiation coupled with GNPs in multiple cell types, we demonstrate decreased head-and-neck cancer (HNC) cell viability in vitro and enhanced cell-killing in zebrafish xenografts compared to standard RT. HNC cell lines also displayed increased double-stranded DNA breaks with DTB irradiation in the presence of GNPs. This study presents preclinical responses to GNP-enhanced radiotherapy with the novel DTB, providing the first functional data to support the theoretical evidence for radiosensitization via GNPs in this context, and highlighting the potential of this approach to optimize the efficacy of RT in anatomically difficult-to-treat tumors. |
format | Online Article Text |
id | pubmed-8799734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-87997342022-02-01 Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis Piccolo, Olivia Lincoln, John D. Melong, Nicole Orr, Benno C. Fernandez, Nicholas R. Borsavage, Jennifer Berman, Jason N. Robar, James Ha, Michael N. Sci Rep Article Radiotherapy (RT) is an effective cancer treatment modality, but standard RT often causes collateral damage to nearby healthy tissues. To increase therapeutic ratio, radiosensitization via gold nanoparticles (GNPs) has been shown to be effective. One challenge is that megavoltage beams generated by clinical linear accelerators are poor initiators of the photoelectric effect. Previous computer models predicted that a diamond target beam (DTB) will yield 400% more low-energy photons, increasing the probability of interacting with GNPs to enhance the radiation dose by 7.7-fold in the GNP vicinity. After testing DTB radiation coupled with GNPs in multiple cell types, we demonstrate decreased head-and-neck cancer (HNC) cell viability in vitro and enhanced cell-killing in zebrafish xenografts compared to standard RT. HNC cell lines also displayed increased double-stranded DNA breaks with DTB irradiation in the presence of GNPs. This study presents preclinical responses to GNP-enhanced radiotherapy with the novel DTB, providing the first functional data to support the theoretical evidence for radiosensitization via GNPs in this context, and highlighting the potential of this approach to optimize the efficacy of RT in anatomically difficult-to-treat tumors. Nature Publishing Group UK 2022-01-28 /pmc/articles/PMC8799734/ /pubmed/35091583 http://dx.doi.org/10.1038/s41598-022-05339-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Piccolo, Olivia Lincoln, John D. Melong, Nicole Orr, Benno C. Fernandez, Nicholas R. Borsavage, Jennifer Berman, Jason N. Robar, James Ha, Michael N. Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
title | Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
title_full | Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
title_fullStr | Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
title_full_unstemmed | Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
title_short | Radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
title_sort | radiation dose enhancement using gold nanoparticles with a diamond linear accelerator target: a multiple cell type analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799734/ https://www.ncbi.nlm.nih.gov/pubmed/35091583 http://dx.doi.org/10.1038/s41598-022-05339-z |
work_keys_str_mv | AT piccoloolivia radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT lincolnjohnd radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT melongnicole radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT orrbennoc radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT fernandeznicholasr radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT borsavagejennifer radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT bermanjasonn radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT robarjames radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis AT hamichaeln radiationdoseenhancementusinggoldnanoparticleswithadiamondlinearacceleratortargetamultiplecelltypeanalysis |