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Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean
Castor bean (Ricinus communis L.) is an important cultivated oilseed. Seeds contain ricinoleic acid, a valuable product for a variety of industries. Castor cake is a residue of ricinoleic manufacture and could be used as animal feed due to its high amount of protein. However, castor cake contains ri...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799790/ https://www.ncbi.nlm.nih.gov/pubmed/35089467 http://dx.doi.org/10.1186/s43141-022-00303-w |
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author | de Sousa, Natália L. Cabral, Glaucia B. Aragão, Francisco J. L. |
author_facet | de Sousa, Natália L. Cabral, Glaucia B. Aragão, Francisco J. L. |
author_sort | de Sousa, Natália L. |
collection | PubMed |
description | Castor bean (Ricinus communis L.) is an important cultivated oilseed. Seeds contain ricinoleic acid, a valuable product for a variety of industries. Castor cake is a residue of ricinoleic manufacture and could be used as animal feed due to its high amount of protein. However, castor cake contains ricin and RCA(120), both highly toxic and allergenic proteins. In 2017, we reported the development of a transgenic event (named TB14S-5D) with an undetectable amount of ricin/RCA(120.) In the present work, we evaluate TB14S-5D for tolerance to the herbicide imazapyr, as it contains the selectable marker gene, ahas, which was previously isolated from Arabidopsis thaliana and contains a mutation at position 653 bp. In addition, we demonstrated that the ricin coding genes are stably silenced over three generations. |
format | Online Article Text |
id | pubmed-8799790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-87997902022-02-07 Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean de Sousa, Natália L. Cabral, Glaucia B. Aragão, Francisco J. L. J Genet Eng Biotechnol Short Communications Castor bean (Ricinus communis L.) is an important cultivated oilseed. Seeds contain ricinoleic acid, a valuable product for a variety of industries. Castor cake is a residue of ricinoleic manufacture and could be used as animal feed due to its high amount of protein. However, castor cake contains ricin and RCA(120), both highly toxic and allergenic proteins. In 2017, we reported the development of a transgenic event (named TB14S-5D) with an undetectable amount of ricin/RCA(120.) In the present work, we evaluate TB14S-5D for tolerance to the herbicide imazapyr, as it contains the selectable marker gene, ahas, which was previously isolated from Arabidopsis thaliana and contains a mutation at position 653 bp. In addition, we demonstrated that the ricin coding genes are stably silenced over three generations. Springer Berlin Heidelberg 2022-01-28 /pmc/articles/PMC8799790/ /pubmed/35089467 http://dx.doi.org/10.1186/s43141-022-00303-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Short Communications de Sousa, Natália L. Cabral, Glaucia B. Aragão, Francisco J. L. Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
title | Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
title_full | Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
title_fullStr | Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
title_full_unstemmed | Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
title_short | Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
title_sort | herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8799790/ https://www.ncbi.nlm.nih.gov/pubmed/35089467 http://dx.doi.org/10.1186/s43141-022-00303-w |
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