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Saliva as a non-invasive specimen for COPD assessment

BACKGROUND: People with COPD have been reported to bear a distinct airway microbiota from healthy individuals based on bronchoalveolar lavage (BAL) and sputum samples. Unfortunately, the collection of these samples involves relatively invasive procedures and is resource-demanding, limiting its regul...

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Autores principales: Melo-Dias, Sara, Valente, Carla, Andrade, Lília, Marques, Alda, Sousa, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8800366/
https://www.ncbi.nlm.nih.gov/pubmed/35093093
http://dx.doi.org/10.1186/s12931-022-01935-9
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author Melo-Dias, Sara
Valente, Carla
Andrade, Lília
Marques, Alda
Sousa, Ana
author_facet Melo-Dias, Sara
Valente, Carla
Andrade, Lília
Marques, Alda
Sousa, Ana
author_sort Melo-Dias, Sara
collection PubMed
description BACKGROUND: People with COPD have been reported to bear a distinct airway microbiota from healthy individuals based on bronchoalveolar lavage (BAL) and sputum samples. Unfortunately, the collection of these samples involves relatively invasive procedures and is resource-demanding, limiting its regular use. Non-invasive samples from the upper airways could constitute an interesting alternative, but its relationship with COPD is still underexplored. We examined the merits of saliva to identify the typical profile of COPD oral bacteria and test its association with the disease. METHODS: Outpatients with COPD and age-sex matched healthy controls were recruited and characterised based on clinical parameters and 16S rRNA profiling of oral bacteria. A clustering analysis based on patients’ oral bacteria beta-diversity and logistic regressions were performed to evaluate the association between oral bacteria composition and COPD. RESULTS: 128 individuals participated (70 patients and 58 controls). Differential abundance analyses showed differences in patients comparable to the ones previously observed in samples from the lower respiratory tract, i.e., an increase in Proteobacteria (particularly Haemophilus) and loss of microbiota diversity. An unsupervised clustering analysis separated patients in two groups based on microbiota composition differing significantly in the frequency of patients hospitalized due to severe acute exacerbation of COPD (AECOPD) and in the frequency of GOLD D patients. Furthermore, a low frequency of Prevotella was associated with a significantly higher risk of recent severe AECOPD and of being GOLD D. CONCLUSION: Salivary bacteria showed an association with COPD, particularly with severe exacerbations, supporting the use of this non-invasive specimen for future studies of heterogeneous respiratory diseases like COPD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-022-01935-9.
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spelling pubmed-88003662022-02-02 Saliva as a non-invasive specimen for COPD assessment Melo-Dias, Sara Valente, Carla Andrade, Lília Marques, Alda Sousa, Ana Respir Res Research BACKGROUND: People with COPD have been reported to bear a distinct airway microbiota from healthy individuals based on bronchoalveolar lavage (BAL) and sputum samples. Unfortunately, the collection of these samples involves relatively invasive procedures and is resource-demanding, limiting its regular use. Non-invasive samples from the upper airways could constitute an interesting alternative, but its relationship with COPD is still underexplored. We examined the merits of saliva to identify the typical profile of COPD oral bacteria and test its association with the disease. METHODS: Outpatients with COPD and age-sex matched healthy controls were recruited and characterised based on clinical parameters and 16S rRNA profiling of oral bacteria. A clustering analysis based on patients’ oral bacteria beta-diversity and logistic regressions were performed to evaluate the association between oral bacteria composition and COPD. RESULTS: 128 individuals participated (70 patients and 58 controls). Differential abundance analyses showed differences in patients comparable to the ones previously observed in samples from the lower respiratory tract, i.e., an increase in Proteobacteria (particularly Haemophilus) and loss of microbiota diversity. An unsupervised clustering analysis separated patients in two groups based on microbiota composition differing significantly in the frequency of patients hospitalized due to severe acute exacerbation of COPD (AECOPD) and in the frequency of GOLD D patients. Furthermore, a low frequency of Prevotella was associated with a significantly higher risk of recent severe AECOPD and of being GOLD D. CONCLUSION: Salivary bacteria showed an association with COPD, particularly with severe exacerbations, supporting the use of this non-invasive specimen for future studies of heterogeneous respiratory diseases like COPD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12931-022-01935-9. BioMed Central 2022-01-29 2022 /pmc/articles/PMC8800366/ /pubmed/35093093 http://dx.doi.org/10.1186/s12931-022-01935-9 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Melo-Dias, Sara
Valente, Carla
Andrade, Lília
Marques, Alda
Sousa, Ana
Saliva as a non-invasive specimen for COPD assessment
title Saliva as a non-invasive specimen for COPD assessment
title_full Saliva as a non-invasive specimen for COPD assessment
title_fullStr Saliva as a non-invasive specimen for COPD assessment
title_full_unstemmed Saliva as a non-invasive specimen for COPD assessment
title_short Saliva as a non-invasive specimen for COPD assessment
title_sort saliva as a non-invasive specimen for copd assessment
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8800366/
https://www.ncbi.nlm.nih.gov/pubmed/35093093
http://dx.doi.org/10.1186/s12931-022-01935-9
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