Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA

BACKGROUND: Recombinase (uvsY and uvsX) from bacteriophage T4 is a key enzyme for recombinase polymerase amplification (RPA) that amplifies a target DNA sequence at a constant temperature with a single-stranded DNA-binding protein and a strand-displacing polymerase. The present study was conducted t...

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Detalles Bibliográficos
Autores principales: Juma, Kevin Maafu, Takita, Teisuke, Yamagata, Masaya, Ishitani, Mika, Hayashi, Kaichi, Kojima, Kenji, Suzuki, Koichiro, Ando, Yuri, Fukuda, Wakao, Fujiwara, Shinsuke, Nakura, Yukiko, Yanagihara, Itaru, Yasukawa, Kiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801280/
https://www.ncbi.nlm.nih.gov/pubmed/35098395
http://dx.doi.org/10.1007/s11033-021-07098-y

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