Functional genetic dropout screens and in vivo validation of candidate therapeutic targets using mouse mammary tumoroids

We have previously developed 3D tumoroids derived from genetically engineered mouse models (GEMMs) of BRCA1/2-deficient breast cancer. Here, we describe how to genetically modify tumoroids, use them for functional genetic dropout screens, and carry out orthotopic transplantation of modified tumoroid...

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Detalles Bibliográficos
Autores principales: Paes Dias, Mariana, Rottenberg, Sven, Jonkers, Jos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801381/
https://www.ncbi.nlm.nih.gov/pubmed/35146447
http://dx.doi.org/10.1016/j.xpro.2022.101132

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801381/
https://www.ncbi.nlm.nih.gov/pubmed/35146447
http://dx.doi.org/10.1016/j.xpro.2022.101132

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