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Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration

The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of sof...

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Autores principales: Miranda, Anamar, Seyer, Damien, Palomino-Durand, Carla, Morakchi-Goudjil, Houda, Massonie, Mathilde, Agniel, Rémy, Rammal, Hassan, Pauthe, Emmanuel, Gand, Adeline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801876/
https://www.ncbi.nlm.nih.gov/pubmed/35111738
http://dx.doi.org/10.3389/fbioe.2021.807697
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author Miranda, Anamar
Seyer, Damien
Palomino-Durand, Carla
Morakchi-Goudjil, Houda
Massonie, Mathilde
Agniel, Rémy
Rammal, Hassan
Pauthe, Emmanuel
Gand, Adeline
author_facet Miranda, Anamar
Seyer, Damien
Palomino-Durand, Carla
Morakchi-Goudjil, Houda
Massonie, Mathilde
Agniel, Rémy
Rammal, Hassan
Pauthe, Emmanuel
Gand, Adeline
author_sort Miranda, Anamar
collection PubMed
description The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of soft tissue onto the implant, while preventing potential early and late infection. Indeed, implant rejection is often jeopardized by lack of stable tissue surrounding the biomaterial combined with infections which reduce the lifespan and increase the failure rate of implants and morbidity and account for high medical costs. Thin films formed by the layer-by-layer (LbL) assembly of oppositely charged polyelectrolytes are particularly versatile and attractive for applications involving cell–material contact. With the combination of the extracellular matrix protein fibronectin (Fn, purified from human plasma) and poly-L-lysine (PLL, exhibiting specific chain lengths), we proposed proactive and biomimetic coatings able to guarantee enhanced cell attachment and exhibiting antimicrobial properties. Fn, able to create a biomimetic interface that could enhance cell attachment and promote extracellular cell matrix remodeling, is incorporated as the anionic polymer during film construction by the LbL technic whereas PLL is used as the cationic polymer for its capacity to confer remarkable antibacterial properties.
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spelling pubmed-88018762022-02-01 Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration Miranda, Anamar Seyer, Damien Palomino-Durand, Carla Morakchi-Goudjil, Houda Massonie, Mathilde Agniel, Rémy Rammal, Hassan Pauthe, Emmanuel Gand, Adeline Front Bioeng Biotechnol Bioengineering and Biotechnology The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of soft tissue onto the implant, while preventing potential early and late infection. Indeed, implant rejection is often jeopardized by lack of stable tissue surrounding the biomaterial combined with infections which reduce the lifespan and increase the failure rate of implants and morbidity and account for high medical costs. Thin films formed by the layer-by-layer (LbL) assembly of oppositely charged polyelectrolytes are particularly versatile and attractive for applications involving cell–material contact. With the combination of the extracellular matrix protein fibronectin (Fn, purified from human plasma) and poly-L-lysine (PLL, exhibiting specific chain lengths), we proposed proactive and biomimetic coatings able to guarantee enhanced cell attachment and exhibiting antimicrobial properties. Fn, able to create a biomimetic interface that could enhance cell attachment and promote extracellular cell matrix remodeling, is incorporated as the anionic polymer during film construction by the LbL technic whereas PLL is used as the cationic polymer for its capacity to confer remarkable antibacterial properties. Frontiers Media S.A. 2022-01-17 /pmc/articles/PMC8801876/ /pubmed/35111738 http://dx.doi.org/10.3389/fbioe.2021.807697 Text en Copyright © 2022 Miranda, Seyer, Palomino-Durand, Morakchi-Goudjil, Massonie, Agniel, Rammal, Pauthe and Gand. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Miranda, Anamar
Seyer, Damien
Palomino-Durand, Carla
Morakchi-Goudjil, Houda
Massonie, Mathilde
Agniel, Rémy
Rammal, Hassan
Pauthe, Emmanuel
Gand, Adeline
Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
title Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
title_full Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
title_fullStr Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
title_full_unstemmed Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
title_short Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
title_sort poly-l-lysine and human plasmatic fibronectin films as proactive coatings to improve implant biointegration
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801876/
https://www.ncbi.nlm.nih.gov/pubmed/35111738
http://dx.doi.org/10.3389/fbioe.2021.807697
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