Cargando…
Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration
The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of sof...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801876/ https://www.ncbi.nlm.nih.gov/pubmed/35111738 http://dx.doi.org/10.3389/fbioe.2021.807697 |
_version_ | 1784642551068753920 |
---|---|
author | Miranda, Anamar Seyer, Damien Palomino-Durand, Carla Morakchi-Goudjil, Houda Massonie, Mathilde Agniel, Rémy Rammal, Hassan Pauthe, Emmanuel Gand, Adeline |
author_facet | Miranda, Anamar Seyer, Damien Palomino-Durand, Carla Morakchi-Goudjil, Houda Massonie, Mathilde Agniel, Rémy Rammal, Hassan Pauthe, Emmanuel Gand, Adeline |
author_sort | Miranda, Anamar |
collection | PubMed |
description | The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of soft tissue onto the implant, while preventing potential early and late infection. Indeed, implant rejection is often jeopardized by lack of stable tissue surrounding the biomaterial combined with infections which reduce the lifespan and increase the failure rate of implants and morbidity and account for high medical costs. Thin films formed by the layer-by-layer (LbL) assembly of oppositely charged polyelectrolytes are particularly versatile and attractive for applications involving cell–material contact. With the combination of the extracellular matrix protein fibronectin (Fn, purified from human plasma) and poly-L-lysine (PLL, exhibiting specific chain lengths), we proposed proactive and biomimetic coatings able to guarantee enhanced cell attachment and exhibiting antimicrobial properties. Fn, able to create a biomimetic interface that could enhance cell attachment and promote extracellular cell matrix remodeling, is incorporated as the anionic polymer during film construction by the LbL technic whereas PLL is used as the cationic polymer for its capacity to confer remarkable antibacterial properties. |
format | Online Article Text |
id | pubmed-8801876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88018762022-02-01 Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration Miranda, Anamar Seyer, Damien Palomino-Durand, Carla Morakchi-Goudjil, Houda Massonie, Mathilde Agniel, Rémy Rammal, Hassan Pauthe, Emmanuel Gand, Adeline Front Bioeng Biotechnol Bioengineering and Biotechnology The success of stable and long-term implant integration implies the promotion, control, and respect of the cell microenvironment at the site of implantation. The key is to enhance the implant–host tissue cross talk by developing interfacial strategies that guarantee an optimal and stable seal of soft tissue onto the implant, while preventing potential early and late infection. Indeed, implant rejection is often jeopardized by lack of stable tissue surrounding the biomaterial combined with infections which reduce the lifespan and increase the failure rate of implants and morbidity and account for high medical costs. Thin films formed by the layer-by-layer (LbL) assembly of oppositely charged polyelectrolytes are particularly versatile and attractive for applications involving cell–material contact. With the combination of the extracellular matrix protein fibronectin (Fn, purified from human plasma) and poly-L-lysine (PLL, exhibiting specific chain lengths), we proposed proactive and biomimetic coatings able to guarantee enhanced cell attachment and exhibiting antimicrobial properties. Fn, able to create a biomimetic interface that could enhance cell attachment and promote extracellular cell matrix remodeling, is incorporated as the anionic polymer during film construction by the LbL technic whereas PLL is used as the cationic polymer for its capacity to confer remarkable antibacterial properties. Frontiers Media S.A. 2022-01-17 /pmc/articles/PMC8801876/ /pubmed/35111738 http://dx.doi.org/10.3389/fbioe.2021.807697 Text en Copyright © 2022 Miranda, Seyer, Palomino-Durand, Morakchi-Goudjil, Massonie, Agniel, Rammal, Pauthe and Gand. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Miranda, Anamar Seyer, Damien Palomino-Durand, Carla Morakchi-Goudjil, Houda Massonie, Mathilde Agniel, Rémy Rammal, Hassan Pauthe, Emmanuel Gand, Adeline Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration |
title | Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration |
title_full | Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration |
title_fullStr | Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration |
title_full_unstemmed | Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration |
title_short | Poly-L-Lysine and Human Plasmatic Fibronectin Films as Proactive Coatings to Improve Implant Biointegration |
title_sort | poly-l-lysine and human plasmatic fibronectin films as proactive coatings to improve implant biointegration |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801876/ https://www.ncbi.nlm.nih.gov/pubmed/35111738 http://dx.doi.org/10.3389/fbioe.2021.807697 |
work_keys_str_mv | AT mirandaanamar polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT seyerdamien polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT palominodurandcarla polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT morakchigoudjilhouda polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT massoniemathilde polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT agnielremy polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT rammalhassan polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT pautheemmanuel polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration AT gandadeline polyllysineandhumanplasmaticfibronectinfilmsasproactivecoatingstoimproveimplantbiointegration |