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Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children
Severe mycoplasma pneumoniae pneumonia (MPP) in children presents with serious clinical complications. Without proper and prompt intervention, it could lead to deadly consequences. Dynamics of the inflammatory airway milieu and activation status of immune cells were believed to be the hallmark of th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801936/ https://www.ncbi.nlm.nih.gov/pubmed/35111152 http://dx.doi.org/10.3389/fimmu.2021.760488 |
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author | Chen, Xi Liu, Fang Zheng, Baoying Kang, Xiaohui Wang, Xiaolin Mou, Wenjun Zhang, Hui Jiao, Anxia Zhao, Shunying Gui, Jingang |
author_facet | Chen, Xi Liu, Fang Zheng, Baoying Kang, Xiaohui Wang, Xiaolin Mou, Wenjun Zhang, Hui Jiao, Anxia Zhao, Shunying Gui, Jingang |
author_sort | Chen, Xi |
collection | PubMed |
description | Severe mycoplasma pneumoniae pneumonia (MPP) in children presents with serious clinical complications. Without proper and prompt intervention, it could lead to deadly consequences. Dynamics of the inflammatory airway milieu and activation status of immune cells were believed to be the hallmark of the pathogenesis and progress of the disease. In this study, by employing the T-cell sorting and mRNA microarray, we were able to define the main feature of the chemokine/cytokine expression and the unique characteristics of T cells in the bronchoalveolar lavage fluid (BALF) from severe MPP patients at acute phase. Our study for the first time delineated the molecular changes in isolated BALF T cells in severe MPP children with respect to the cytokine/chemokine expression, cell activation, exhaustion, and apoptosis. By comparing the BALF aqueous expression of cytokines/chemokines with that in sorted T cells, our data give a preliminary clue capable of finishing out the possible cell source of the proinflammatory cytokines/chemokines from the BALF mixture. Meanwhile, our data provide a distinctively pellucid expression profile particularly belonging to the isolated BALF T cells demonstrating that in the inflammatory airway, overactivated T cells were exhausted and on the verge of apoptotic progress. |
format | Online Article Text |
id | pubmed-8801936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88019362022-02-01 Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children Chen, Xi Liu, Fang Zheng, Baoying Kang, Xiaohui Wang, Xiaolin Mou, Wenjun Zhang, Hui Jiao, Anxia Zhao, Shunying Gui, Jingang Front Immunol Immunology Severe mycoplasma pneumoniae pneumonia (MPP) in children presents with serious clinical complications. Without proper and prompt intervention, it could lead to deadly consequences. Dynamics of the inflammatory airway milieu and activation status of immune cells were believed to be the hallmark of the pathogenesis and progress of the disease. In this study, by employing the T-cell sorting and mRNA microarray, we were able to define the main feature of the chemokine/cytokine expression and the unique characteristics of T cells in the bronchoalveolar lavage fluid (BALF) from severe MPP patients at acute phase. Our study for the first time delineated the molecular changes in isolated BALF T cells in severe MPP children with respect to the cytokine/chemokine expression, cell activation, exhaustion, and apoptosis. By comparing the BALF aqueous expression of cytokines/chemokines with that in sorted T cells, our data give a preliminary clue capable of finishing out the possible cell source of the proinflammatory cytokines/chemokines from the BALF mixture. Meanwhile, our data provide a distinctively pellucid expression profile particularly belonging to the isolated BALF T cells demonstrating that in the inflammatory airway, overactivated T cells were exhausted and on the verge of apoptotic progress. Frontiers Media S.A. 2022-01-17 /pmc/articles/PMC8801936/ /pubmed/35111152 http://dx.doi.org/10.3389/fimmu.2021.760488 Text en Copyright © 2022 Chen, Liu, Zheng, Kang, Wang, Mou, Zhang, Jiao, Zhao and Gui https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Chen, Xi Liu, Fang Zheng, Baoying Kang, Xiaohui Wang, Xiaolin Mou, Wenjun Zhang, Hui Jiao, Anxia Zhao, Shunying Gui, Jingang Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children |
title | Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children |
title_full | Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children |
title_fullStr | Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children |
title_full_unstemmed | Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children |
title_short | Exhausted and Apoptotic BALF T Cells in Proinflammatory Airway Milieu at Acute Phase of Severe Mycoplasma Pneumoniae Pneumonia in Children |
title_sort | exhausted and apoptotic balf t cells in proinflammatory airway milieu at acute phase of severe mycoplasma pneumoniae pneumonia in children |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8801936/ https://www.ncbi.nlm.nih.gov/pubmed/35111152 http://dx.doi.org/10.3389/fimmu.2021.760488 |
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