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Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
[Image: see text] Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802189/ https://www.ncbi.nlm.nih.gov/pubmed/35111902 http://dx.doi.org/10.1021/acscentsci.1c01153 |
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author | Grenier, Vincent Martinez, Kayli N. Benlian, Brittany R. García-Almedina, Derek M. Raliski, Benjamin K. Boggess, Steven C. Maza, Johnathan C. Yang, Samantha J. Gest, Anneliese M. M. Miller, Evan W. |
author_facet | Grenier, Vincent Martinez, Kayli N. Benlian, Brittany R. García-Almedina, Derek M. Raliski, Benjamin K. Boggess, Steven C. Maza, Johnathan C. Yang, Samantha J. Gest, Anneliese M. M. Miller, Evan W. |
author_sort | Grenier, Vincent |
collection | PubMed |
description | [Image: see text] Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes. |
format | Online Article Text |
id | pubmed-8802189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-88021892022-02-01 Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters Grenier, Vincent Martinez, Kayli N. Benlian, Brittany R. García-Almedina, Derek M. Raliski, Benjamin K. Boggess, Steven C. Maza, Johnathan C. Yang, Samantha J. Gest, Anneliese M. M. Miller, Evan W. ACS Cent Sci [Image: see text] Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes. American Chemical Society 2022-01-13 2022-01-26 /pmc/articles/PMC8802189/ /pubmed/35111902 http://dx.doi.org/10.1021/acscentsci.1c01153 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Grenier, Vincent Martinez, Kayli N. Benlian, Brittany R. García-Almedina, Derek M. Raliski, Benjamin K. Boggess, Steven C. Maza, Johnathan C. Yang, Samantha J. Gest, Anneliese M. M. Miller, Evan W. Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters |
title | Molecular Prosthetics for Long-Term Functional Imaging
with Fluorescent Reporters |
title_full | Molecular Prosthetics for Long-Term Functional Imaging
with Fluorescent Reporters |
title_fullStr | Molecular Prosthetics for Long-Term Functional Imaging
with Fluorescent Reporters |
title_full_unstemmed | Molecular Prosthetics for Long-Term Functional Imaging
with Fluorescent Reporters |
title_short | Molecular Prosthetics for Long-Term Functional Imaging
with Fluorescent Reporters |
title_sort | molecular prosthetics for long-term functional imaging
with fluorescent reporters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802189/ https://www.ncbi.nlm.nih.gov/pubmed/35111902 http://dx.doi.org/10.1021/acscentsci.1c01153 |
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