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Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters

[Image: see text] Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of...

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Autores principales: Grenier, Vincent, Martinez, Kayli N., Benlian, Brittany R., García-Almedina, Derek M., Raliski, Benjamin K., Boggess, Steven C., Maza, Johnathan C., Yang, Samantha J., Gest, Anneliese M. M., Miller, Evan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802189/
https://www.ncbi.nlm.nih.gov/pubmed/35111902
http://dx.doi.org/10.1021/acscentsci.1c01153
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author Grenier, Vincent
Martinez, Kayli N.
Benlian, Brittany R.
García-Almedina, Derek M.
Raliski, Benjamin K.
Boggess, Steven C.
Maza, Johnathan C.
Yang, Samantha J.
Gest, Anneliese M. M.
Miller, Evan W.
author_facet Grenier, Vincent
Martinez, Kayli N.
Benlian, Brittany R.
García-Almedina, Derek M.
Raliski, Benjamin K.
Boggess, Steven C.
Maza, Johnathan C.
Yang, Samantha J.
Gest, Anneliese M. M.
Miller, Evan W.
author_sort Grenier, Vincent
collection PubMed
description [Image: see text] Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes.
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spelling pubmed-88021892022-02-01 Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters Grenier, Vincent Martinez, Kayli N. Benlian, Brittany R. García-Almedina, Derek M. Raliski, Benjamin K. Boggess, Steven C. Maza, Johnathan C. Yang, Samantha J. Gest, Anneliese M. M. Miller, Evan W. ACS Cent Sci [Image: see text] Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes. American Chemical Society 2022-01-13 2022-01-26 /pmc/articles/PMC8802189/ /pubmed/35111902 http://dx.doi.org/10.1021/acscentsci.1c01153 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Grenier, Vincent
Martinez, Kayli N.
Benlian, Brittany R.
García-Almedina, Derek M.
Raliski, Benjamin K.
Boggess, Steven C.
Maza, Johnathan C.
Yang, Samantha J.
Gest, Anneliese M. M.
Miller, Evan W.
Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
title Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
title_full Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
title_fullStr Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
title_full_unstemmed Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
title_short Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
title_sort molecular prosthetics for long-term functional imaging with fluorescent reporters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802189/
https://www.ncbi.nlm.nih.gov/pubmed/35111902
http://dx.doi.org/10.1021/acscentsci.1c01153
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