Optofluidic flow meter for sub-nanoliter per minute flow measurements

SIGNIFICANCE: Performance improvements in microfluidic systems depend on accurate measurement and fluid control on the micro- and nanoscales. New applications are continuously leading to lower volumetric flow rates. AIM: We focus on improving an optofluidic system for measuring and calibrating micro...

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Autores principales: Sadeghi, Jalal, Patrone, Paul N., Kearsley, Anthony J., Cooksey, Gregory A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802908/
https://www.ncbi.nlm.nih.gov/pubmed/35102729
http://dx.doi.org/10.1117/1.JBO.27.1.017001
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author Sadeghi, Jalal
Patrone, Paul N.
Kearsley, Anthony J.
Cooksey, Gregory A.
author_facet Sadeghi, Jalal
Patrone, Paul N.
Kearsley, Anthony J.
Cooksey, Gregory A.
author_sort Sadeghi, Jalal
collection PubMed
description SIGNIFICANCE: Performance improvements in microfluidic systems depend on accurate measurement and fluid control on the micro- and nanoscales. New applications are continuously leading to lower volumetric flow rates. AIM: We focus on improving an optofluidic system for measuring and calibrating microflows to the sub-nanoliter per minute range. APPROACH: Measurements rely on an optofluidic system that delivers excitation light and records fluorescence in a precise interrogation region of a microfluidic channel. Exploiting a scaling relationship between the flow rate and fluorescence emission after photobleaching, the system enables real-time determination of flow rates. RESULTS: Here, we demonstrate improved calibration of a flow controller to 1% uncertainty. Further, the resolution of the optofluidic flow meter improved to less than [Formula: see text] with 5% uncertainty using a molecule with a 14-fold smaller diffusion coefficient than our previous report. CONCLUSIONS: We demonstrate new capabilities in sub-nanoliter per minute flow control and measurement that are generalizable to cutting-edge light-material interaction and molecular diffusion for chemical and biomedical industries.
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spelling pubmed-88029082022-02-02 Optofluidic flow meter for sub-nanoliter per minute flow measurements Sadeghi, Jalal Patrone, Paul N. Kearsley, Anthony J. Cooksey, Gregory A. J Biomed Opt Sensing SIGNIFICANCE: Performance improvements in microfluidic systems depend on accurate measurement and fluid control on the micro- and nanoscales. New applications are continuously leading to lower volumetric flow rates. AIM: We focus on improving an optofluidic system for measuring and calibrating microflows to the sub-nanoliter per minute range. APPROACH: Measurements rely on an optofluidic system that delivers excitation light and records fluorescence in a precise interrogation region of a microfluidic channel. Exploiting a scaling relationship between the flow rate and fluorescence emission after photobleaching, the system enables real-time determination of flow rates. RESULTS: Here, we demonstrate improved calibration of a flow controller to 1% uncertainty. Further, the resolution of the optofluidic flow meter improved to less than [Formula: see text] with 5% uncertainty using a molecule with a 14-fold smaller diffusion coefficient than our previous report. CONCLUSIONS: We demonstrate new capabilities in sub-nanoliter per minute flow control and measurement that are generalizable to cutting-edge light-material interaction and molecular diffusion for chemical and biomedical industries. Society of Photo-Optical Instrumentation Engineers 2022-01-31 2022-01 /pmc/articles/PMC8802908/ /pubmed/35102729 http://dx.doi.org/10.1117/1.JBO.27.1.017001 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Sensing
Sadeghi, Jalal
Patrone, Paul N.
Kearsley, Anthony J.
Cooksey, Gregory A.
Optofluidic flow meter for sub-nanoliter per minute flow measurements
title Optofluidic flow meter for sub-nanoliter per minute flow measurements
title_full Optofluidic flow meter for sub-nanoliter per minute flow measurements
title_fullStr Optofluidic flow meter for sub-nanoliter per minute flow measurements
title_full_unstemmed Optofluidic flow meter for sub-nanoliter per minute flow measurements
title_short Optofluidic flow meter for sub-nanoliter per minute flow measurements
title_sort optofluidic flow meter for sub-nanoliter per minute flow measurements
topic Sensing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802908/
https://www.ncbi.nlm.nih.gov/pubmed/35102729
http://dx.doi.org/10.1117/1.JBO.27.1.017001
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