Optofluidic flow meter for sub-nanoliter per minute flow measurements
SIGNIFICANCE: Performance improvements in microfluidic systems depend on accurate measurement and fluid control on the micro- and nanoscales. New applications are continuously leading to lower volumetric flow rates. AIM: We focus on improving an optofluidic system for measuring and calibrating micro...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802908/ https://www.ncbi.nlm.nih.gov/pubmed/35102729 http://dx.doi.org/10.1117/1.JBO.27.1.017001 |
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author | Sadeghi, Jalal Patrone, Paul N. Kearsley, Anthony J. Cooksey, Gregory A. |
author_facet | Sadeghi, Jalal Patrone, Paul N. Kearsley, Anthony J. Cooksey, Gregory A. |
author_sort | Sadeghi, Jalal |
collection | PubMed |
description | SIGNIFICANCE: Performance improvements in microfluidic systems depend on accurate measurement and fluid control on the micro- and nanoscales. New applications are continuously leading to lower volumetric flow rates. AIM: We focus on improving an optofluidic system for measuring and calibrating microflows to the sub-nanoliter per minute range. APPROACH: Measurements rely on an optofluidic system that delivers excitation light and records fluorescence in a precise interrogation region of a microfluidic channel. Exploiting a scaling relationship between the flow rate and fluorescence emission after photobleaching, the system enables real-time determination of flow rates. RESULTS: Here, we demonstrate improved calibration of a flow controller to 1% uncertainty. Further, the resolution of the optofluidic flow meter improved to less than [Formula: see text] with 5% uncertainty using a molecule with a 14-fold smaller diffusion coefficient than our previous report. CONCLUSIONS: We demonstrate new capabilities in sub-nanoliter per minute flow control and measurement that are generalizable to cutting-edge light-material interaction and molecular diffusion for chemical and biomedical industries. |
format | Online Article Text |
id | pubmed-8802908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-88029082022-02-02 Optofluidic flow meter for sub-nanoliter per minute flow measurements Sadeghi, Jalal Patrone, Paul N. Kearsley, Anthony J. Cooksey, Gregory A. J Biomed Opt Sensing SIGNIFICANCE: Performance improvements in microfluidic systems depend on accurate measurement and fluid control on the micro- and nanoscales. New applications are continuously leading to lower volumetric flow rates. AIM: We focus on improving an optofluidic system for measuring and calibrating microflows to the sub-nanoliter per minute range. APPROACH: Measurements rely on an optofluidic system that delivers excitation light and records fluorescence in a precise interrogation region of a microfluidic channel. Exploiting a scaling relationship between the flow rate and fluorescence emission after photobleaching, the system enables real-time determination of flow rates. RESULTS: Here, we demonstrate improved calibration of a flow controller to 1% uncertainty. Further, the resolution of the optofluidic flow meter improved to less than [Formula: see text] with 5% uncertainty using a molecule with a 14-fold smaller diffusion coefficient than our previous report. CONCLUSIONS: We demonstrate new capabilities in sub-nanoliter per minute flow control and measurement that are generalizable to cutting-edge light-material interaction and molecular diffusion for chemical and biomedical industries. Society of Photo-Optical Instrumentation Engineers 2022-01-31 2022-01 /pmc/articles/PMC8802908/ /pubmed/35102729 http://dx.doi.org/10.1117/1.JBO.27.1.017001 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | Sensing Sadeghi, Jalal Patrone, Paul N. Kearsley, Anthony J. Cooksey, Gregory A. Optofluidic flow meter for sub-nanoliter per minute flow measurements |
title | Optofluidic flow meter for sub-nanoliter per minute flow measurements |
title_full | Optofluidic flow meter for sub-nanoliter per minute flow measurements |
title_fullStr | Optofluidic flow meter for sub-nanoliter per minute flow measurements |
title_full_unstemmed | Optofluidic flow meter for sub-nanoliter per minute flow measurements |
title_short | Optofluidic flow meter for sub-nanoliter per minute flow measurements |
title_sort | optofluidic flow meter for sub-nanoliter per minute flow measurements |
topic | Sensing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8802908/ https://www.ncbi.nlm.nih.gov/pubmed/35102729 http://dx.doi.org/10.1117/1.JBO.27.1.017001 |
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