A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath

Changes in adipose tissue morphology, depicted by cell morphology alterations such as enlargement of fat cells, always accompany over-weight and obesity. The variables related to cell size have been shown to associate with low-grade inflammation of adipose tissue and common obesity-related comorbidi...

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Autores principales: Palomäki, Ville A, Koivukangas, Vesa, Meriläinen, Sanna, Lehenkari, Petri, Karttunen, Tuomo J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803053/
https://www.ncbi.nlm.nih.gov/pubmed/35094637
http://dx.doi.org/10.1080/21623945.2022.2027610
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author Palomäki, Ville A
Koivukangas, Vesa
Meriläinen, Sanna
Lehenkari, Petri
Karttunen, Tuomo J
author_facet Palomäki, Ville A
Koivukangas, Vesa
Meriläinen, Sanna
Lehenkari, Petri
Karttunen, Tuomo J
author_sort Palomäki, Ville A
collection PubMed
description Changes in adipose tissue morphology, depicted by cell morphology alterations such as enlargement of fat cells, always accompany over-weight and obesity. The variables related to cell size have been shown to associate with low-grade inflammation of adipose tissue and common obesity-related comorbidities including metabolic syndrome and type 2 diabetes. Quantifying fat cell morphology from images of histological specimens can be tedious. Here, we present a straightforward method for the task using the free open-source software QuPath with its inbuilt tools only. Measurements of human adipose tissue samples with the described protocol showed an excellent correlation with those obtained with ImageJ software with Adipocyte Tools plugin combined with manual correction of misdetections. Intraclass correlation between the two methods was at good to excellent level. The method described here can be applied to considerably large tissue areas, even whole-slide analysis.
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spelling pubmed-88030532022-02-01 A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath Palomäki, Ville A Koivukangas, Vesa Meriläinen, Sanna Lehenkari, Petri Karttunen, Tuomo J Adipocyte Research Paper Changes in adipose tissue morphology, depicted by cell morphology alterations such as enlargement of fat cells, always accompany over-weight and obesity. The variables related to cell size have been shown to associate with low-grade inflammation of adipose tissue and common obesity-related comorbidities including metabolic syndrome and type 2 diabetes. Quantifying fat cell morphology from images of histological specimens can be tedious. Here, we present a straightforward method for the task using the free open-source software QuPath with its inbuilt tools only. Measurements of human adipose tissue samples with the described protocol showed an excellent correlation with those obtained with ImageJ software with Adipocyte Tools plugin combined with manual correction of misdetections. Intraclass correlation between the two methods was at good to excellent level. The method described here can be applied to considerably large tissue areas, even whole-slide analysis. Taylor & Francis 2022-01-30 /pmc/articles/PMC8803053/ /pubmed/35094637 http://dx.doi.org/10.1080/21623945.2022.2027610 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Palomäki, Ville A
Koivukangas, Vesa
Meriläinen, Sanna
Lehenkari, Petri
Karttunen, Tuomo J
A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath
title A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath
title_full A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath
title_fullStr A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath
title_full_unstemmed A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath
title_short A Straightforward Method for Adipocyte Size and Count Analysis Using Open-source Software QuPath
title_sort straightforward method for adipocyte size and count analysis using open-source software qupath
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803053/
https://www.ncbi.nlm.nih.gov/pubmed/35094637
http://dx.doi.org/10.1080/21623945.2022.2027610
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