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Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization

Tumor-associated macrophage (TAM) is a major component of tumor microenvironment (TME) and plays critical role in the progression of cancer metastasis. However, TAM-mediated regulation in gallbladder cancer (GBC) has not been fully characterized. Here, we found that exosomes derived from GBC cell po...

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Autores principales: Zhao, Songling, Liu, Yunxia, He, Linhai, Li, Yuehua, Lin, Ke, Kang, Qiang, Liu, Lixin, Zou, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803447/
https://www.ncbi.nlm.nih.gov/pubmed/35111566
http://dx.doi.org/10.1155/2022/9994906
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author Zhao, Songling
Liu, Yunxia
He, Linhai
Li, Yuehua
Lin, Ke
Kang, Qiang
Liu, Lixin
Zou, Hao
author_facet Zhao, Songling
Liu, Yunxia
He, Linhai
Li, Yuehua
Lin, Ke
Kang, Qiang
Liu, Lixin
Zou, Hao
author_sort Zhao, Songling
collection PubMed
description Tumor-associated macrophage (TAM) is a major component of tumor microenvironment (TME) and plays critical role in the progression of cancer metastasis. However, TAM-mediated regulation in gallbladder cancer (GBC) has not been fully characterized. Here, we found that exosomes derived from GBC cell polarized macrophage to M2 phenotype, which then facilitated the invasion and migration of GBC cells. We discovered that leptin was enriched in GBC cell-derived exosomes. Exosomal leptin levels promoted invasion and migration of GBC-SD cells. The inhibition of leptin not only attenuated M2 macrophage of polarization but also inhibited the invasive and migratory ability of GBC cell. In addition, GBC-SD cell-derived exosomal leptin induced M2 polarization of macrophage via activation of STAT3 signal pathway. Taken together, our results suggested that GBC cells secrete exosome-enclosed leptin facilitated cell invasion and migration via polarizing TAM.
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spelling pubmed-88034472022-02-01 Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization Zhao, Songling Liu, Yunxia He, Linhai Li, Yuehua Lin, Ke Kang, Qiang Liu, Lixin Zou, Hao Anal Cell Pathol (Amst) Research Article Tumor-associated macrophage (TAM) is a major component of tumor microenvironment (TME) and plays critical role in the progression of cancer metastasis. However, TAM-mediated regulation in gallbladder cancer (GBC) has not been fully characterized. Here, we found that exosomes derived from GBC cell polarized macrophage to M2 phenotype, which then facilitated the invasion and migration of GBC cells. We discovered that leptin was enriched in GBC cell-derived exosomes. Exosomal leptin levels promoted invasion and migration of GBC-SD cells. The inhibition of leptin not only attenuated M2 macrophage of polarization but also inhibited the invasive and migratory ability of GBC cell. In addition, GBC-SD cell-derived exosomal leptin induced M2 polarization of macrophage via activation of STAT3 signal pathway. Taken together, our results suggested that GBC cells secrete exosome-enclosed leptin facilitated cell invasion and migration via polarizing TAM. Hindawi 2022-01-24 /pmc/articles/PMC8803447/ /pubmed/35111566 http://dx.doi.org/10.1155/2022/9994906 Text en Copyright © 2022 Songling Zhao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Songling
Liu, Yunxia
He, Linhai
Li, Yuehua
Lin, Ke
Kang, Qiang
Liu, Lixin
Zou, Hao
Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization
title Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization
title_full Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization
title_fullStr Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization
title_full_unstemmed Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization
title_short Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization
title_sort gallbladder cancer cell-derived exosome-mediated transfer of leptin promotes cell invasion and migration by modulating stat3-mediated m2 macrophage polarization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803447/
https://www.ncbi.nlm.nih.gov/pubmed/35111566
http://dx.doi.org/10.1155/2022/9994906
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