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Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay
Diaveridine (DVD) is widely used for the prevention and treatment of coccidiosis and leucocytozoonosis infections in food-producing animals. To gain a better understanding of DVD metabolism and pharmacokinetics in healthy Landrace/Doric Cross castrated male pigs and both female and male Cobb 500 bro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803906/ https://www.ncbi.nlm.nih.gov/pubmed/35118152 http://dx.doi.org/10.3389/fvets.2021.799773 |
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author | Wang, Liye Wen, Lihua Pan, Yuanhu Wang, Zhenzhen Zhou, Kaixiang Mi, Kun Liu, Zhenli Qu, Wei Huang, Lingli |
author_facet | Wang, Liye Wen, Lihua Pan, Yuanhu Wang, Zhenzhen Zhou, Kaixiang Mi, Kun Liu, Zhenli Qu, Wei Huang, Lingli |
author_sort | Wang, Liye |
collection | PubMed |
description | Diaveridine (DVD) is widely used for the prevention and treatment of coccidiosis and leucocytozoonosis infections in food-producing animals. To gain a better understanding of DVD metabolism and pharmacokinetics in healthy Landrace/Doric Cross castrated male pigs and both female and male Cobb 500 broiler chickens, a method involving radioactive tracing coupled with LC/MS-IT-TOF was developed for the identification and quantitation of DVD and its metabolites in pig and chicken plasma, and then was applied to investigate DVD pharmacokinetics. A simple MCX solid phase extraction procedure was adopted for sample preparation. After a single oral administration of (3)H-DVD (10 mg/kg BW), three radioactive compounds (D0: DVD; D1: 3′-desmethyl-DVD; and D2: monoglucuronide of 3′-desmethyl-DVD) were identified in pig plasma, while only two radioactive compounds (D0 and D2) were identified in chicken plasma. In both species, the C(max) values for all detected compounds were reached at 2 h after dosing. The C(max) order was D2 (1.38 μg/ml) > D0 (0.49 μg/ml) > D1 (0.24 μg/ml) in pigs and D0 (1.55 μg/ml) > D2 (0.27 μg/ml) in chickens. The longer t(1/2) (elimination half-life) of D0 contributed to the slow elimination of DVD-related compounds. The t(1/2β) of D0 in pigs (66.41 h) was significantly longer than that in chickens (48.30 h), but the t(1/2) of total DVD-related metabolites in pigs (42.86 h) was lower than that in chickens (56.11 h). These findings suggested that the metabolism and pharmacokinetics of DVD in pigs and chickens were significantly different, and that this would affect its effectiveness, toxicology, and food safety in these animals. |
format | Online Article Text |
id | pubmed-8803906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88039062022-02-02 Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay Wang, Liye Wen, Lihua Pan, Yuanhu Wang, Zhenzhen Zhou, Kaixiang Mi, Kun Liu, Zhenli Qu, Wei Huang, Lingli Front Vet Sci Veterinary Science Diaveridine (DVD) is widely used for the prevention and treatment of coccidiosis and leucocytozoonosis infections in food-producing animals. To gain a better understanding of DVD metabolism and pharmacokinetics in healthy Landrace/Doric Cross castrated male pigs and both female and male Cobb 500 broiler chickens, a method involving radioactive tracing coupled with LC/MS-IT-TOF was developed for the identification and quantitation of DVD and its metabolites in pig and chicken plasma, and then was applied to investigate DVD pharmacokinetics. A simple MCX solid phase extraction procedure was adopted for sample preparation. After a single oral administration of (3)H-DVD (10 mg/kg BW), three radioactive compounds (D0: DVD; D1: 3′-desmethyl-DVD; and D2: monoglucuronide of 3′-desmethyl-DVD) were identified in pig plasma, while only two radioactive compounds (D0 and D2) were identified in chicken plasma. In both species, the C(max) values for all detected compounds were reached at 2 h after dosing. The C(max) order was D2 (1.38 μg/ml) > D0 (0.49 μg/ml) > D1 (0.24 μg/ml) in pigs and D0 (1.55 μg/ml) > D2 (0.27 μg/ml) in chickens. The longer t(1/2) (elimination half-life) of D0 contributed to the slow elimination of DVD-related compounds. The t(1/2β) of D0 in pigs (66.41 h) was significantly longer than that in chickens (48.30 h), but the t(1/2) of total DVD-related metabolites in pigs (42.86 h) was lower than that in chickens (56.11 h). These findings suggested that the metabolism and pharmacokinetics of DVD in pigs and chickens were significantly different, and that this would affect its effectiveness, toxicology, and food safety in these animals. Frontiers Media S.A. 2022-01-18 /pmc/articles/PMC8803906/ /pubmed/35118152 http://dx.doi.org/10.3389/fvets.2021.799773 Text en Copyright © 2022 Wang, Wen, Pan, Wang, Zhou, Mi, Liu, Qu and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Wang, Liye Wen, Lihua Pan, Yuanhu Wang, Zhenzhen Zhou, Kaixiang Mi, Kun Liu, Zhenli Qu, Wei Huang, Lingli Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay |
title | Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay |
title_full | Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay |
title_fullStr | Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay |
title_full_unstemmed | Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay |
title_short | Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay |
title_sort | metabolite identification and pharmacokinetic behavior of diaveridine in the plasma of pigs and chickens based on radioactive tracing coupled with lc/ms-it-tof assay |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8803906/ https://www.ncbi.nlm.nih.gov/pubmed/35118152 http://dx.doi.org/10.3389/fvets.2021.799773 |
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