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Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway

Nasopharyngeal carcinoma (NPC) is a cancer that occurs in the nasopharynx. Infinite proliferation and distant metastasis are the main characteristics of NPC cells, and the main reason for the current failure of malignant tumor treatment. In this study, by integrating the immunohistochemical, cell tr...

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Autores principales: Li, Tingting, Tian, Yong, Wang, Yixuan, Cui, Zhen, He, Zelai, Wu, Xiao, Zhang, Yajun, Jiang, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8804215/
https://www.ncbi.nlm.nih.gov/pubmed/35117986
http://dx.doi.org/10.3389/fonc.2021.724251
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author Li, Tingting
Tian, Yong
Wang, Yixuan
Cui, Zhen
He, Zelai
Wu, Xiao
Zhang, Yajun
Jiang, Hao
author_facet Li, Tingting
Tian, Yong
Wang, Yixuan
Cui, Zhen
He, Zelai
Wu, Xiao
Zhang, Yajun
Jiang, Hao
author_sort Li, Tingting
collection PubMed
description Nasopharyngeal carcinoma (NPC) is a cancer that occurs in the nasopharynx. Infinite proliferation and distant metastasis are the main characteristics of NPC cells, and the main reason for the current failure of malignant tumor treatment. In this study, by integrating the immunohistochemical, cell transfection, western blot and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis, we observed that the expression of KISS1 and its receptor gene (KISS1R) negatively related with the proliferation of NPC cells. Overexpression of the KISS1 genes in cells reduced cell proliferation, slow down the cell cycle, and increased apoptosis. Additionally, overexpression of these genes significantly increased Liver Kinase B1 (LKB1), phosphorylation of LKB1 and AMPK, indicated by Western blotting. Together, all of these results suggested for the first time that KISS1 and KISS1R suppress the proliferation of NPC cells by activating the LKB1/AMPK pathway, thus revealing a viable indicator for diagnosis of NPC in clinical practice.
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spelling pubmed-88042152022-02-02 Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway Li, Tingting Tian, Yong Wang, Yixuan Cui, Zhen He, Zelai Wu, Xiao Zhang, Yajun Jiang, Hao Front Oncol Oncology Nasopharyngeal carcinoma (NPC) is a cancer that occurs in the nasopharynx. Infinite proliferation and distant metastasis are the main characteristics of NPC cells, and the main reason for the current failure of malignant tumor treatment. In this study, by integrating the immunohistochemical, cell transfection, western blot and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis, we observed that the expression of KISS1 and its receptor gene (KISS1R) negatively related with the proliferation of NPC cells. Overexpression of the KISS1 genes in cells reduced cell proliferation, slow down the cell cycle, and increased apoptosis. Additionally, overexpression of these genes significantly increased Liver Kinase B1 (LKB1), phosphorylation of LKB1 and AMPK, indicated by Western blotting. Together, all of these results suggested for the first time that KISS1 and KISS1R suppress the proliferation of NPC cells by activating the LKB1/AMPK pathway, thus revealing a viable indicator for diagnosis of NPC in clinical practice. Frontiers Media S.A. 2022-01-18 /pmc/articles/PMC8804215/ /pubmed/35117986 http://dx.doi.org/10.3389/fonc.2021.724251 Text en Copyright © 2022 Li, Tian, Wang, Cui, He, Wu, Zhang and Jiang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Tingting
Tian, Yong
Wang, Yixuan
Cui, Zhen
He, Zelai
Wu, Xiao
Zhang, Yajun
Jiang, Hao
Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
title Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
title_full Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
title_fullStr Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
title_full_unstemmed Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
title_short Kiss1 Inhibits the Proliferation of Nasopharyngeal Carcinoma Cells Via Activation of the LKB1/AMPK Pathway
title_sort kiss1 inhibits the proliferation of nasopharyngeal carcinoma cells via activation of the lkb1/ampk pathway
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8804215/
https://www.ncbi.nlm.nih.gov/pubmed/35117986
http://dx.doi.org/10.3389/fonc.2021.724251
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