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Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma

Non-functional pituitary adenomas (NFPAs) are one of the most prevalent pituitary adenoma subtypes. The lack of reliable screening approach for NFPAs for the insidious clinical course usually leads to delays in medical therapy and consequently worse prognosis. Hence, we employed a sequence cohort (p...

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Autores principales: Lyu, Liang, Li, Haiyan, Chen, Cheng, Yu, Yang, Wang, Li, Yin, Senlin, Hu, Yu, Jiang, Shu, Ye, Feng, Zhou, Peizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8804500/
https://www.ncbi.nlm.nih.gov/pubmed/35118066
http://dx.doi.org/10.3389/fcell.2021.771354
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author Lyu, Liang
Li, Haiyan
Chen, Cheng
Yu, Yang
Wang, Li
Yin, Senlin
Hu, Yu
Jiang, Shu
Ye, Feng
Zhou, Peizhi
author_facet Lyu, Liang
Li, Haiyan
Chen, Cheng
Yu, Yang
Wang, Li
Yin, Senlin
Hu, Yu
Jiang, Shu
Ye, Feng
Zhou, Peizhi
author_sort Lyu, Liang
collection PubMed
description Non-functional pituitary adenomas (NFPAs) are one of the most prevalent pituitary adenoma subtypes. The lack of reliable screening approach for NFPAs for the insidious clinical course usually leads to delays in medical therapy and consequently worse prognosis. Hence, we employed a sequence cohort (patient: control, 6:2) and a validation cohort (patient: control, 22:8) to develop a serum exosomal miRNA profile-based method for NFPA screening and prognosis prediction. We found that a total of 1,395 kinds of human miRNA were detected. Compared with healthy donors, 18 up-regulated and 36 down-regulated miRNAs showed significant expression alterations in NFPA patients. Target genes of differentially expressed miRNAs are mainly enriched in axonogenesis and cancer-associated terms. After validation, hsa-miR-486-5p, hsa-miR-151a-5p, hsa-miR-652-3p_R+1, and hsa-miR-1180-3p were promising biomarkers for NFPA, in which miR-486-5p was the most competent one. After a median of 33 months of prospective follow-up, exosomal hsa-miR-486-5p also was an efficient predictive biomarker for progression or relapse of NFPAs. By protein-protein interaction network construction of hsa-miR-486-5p targeted genes, the core modules revealed a high possibility that exosomal hsa-miR-486-5p regulated tumor progression by epigenetic regulation of MAPK signaling pathways. In conclusion, exosomal hsa-miR-486-5p, hsa-miR-151a-5p, hsa-miR-652-3p_R+1, and hsa-miR-1180-3p are candidate biomarkers for diagnosis and screening of NFPAs. More importantly, prospective follow-up reveals that hsa-miR-486-5p can be regarded as a significant predictor for prognosis of NFPAs.
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spelling pubmed-88045002022-02-02 Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma Lyu, Liang Li, Haiyan Chen, Cheng Yu, Yang Wang, Li Yin, Senlin Hu, Yu Jiang, Shu Ye, Feng Zhou, Peizhi Front Cell Dev Biol Cell and Developmental Biology Non-functional pituitary adenomas (NFPAs) are one of the most prevalent pituitary adenoma subtypes. The lack of reliable screening approach for NFPAs for the insidious clinical course usually leads to delays in medical therapy and consequently worse prognosis. Hence, we employed a sequence cohort (patient: control, 6:2) and a validation cohort (patient: control, 22:8) to develop a serum exosomal miRNA profile-based method for NFPA screening and prognosis prediction. We found that a total of 1,395 kinds of human miRNA were detected. Compared with healthy donors, 18 up-regulated and 36 down-regulated miRNAs showed significant expression alterations in NFPA patients. Target genes of differentially expressed miRNAs are mainly enriched in axonogenesis and cancer-associated terms. After validation, hsa-miR-486-5p, hsa-miR-151a-5p, hsa-miR-652-3p_R+1, and hsa-miR-1180-3p were promising biomarkers for NFPA, in which miR-486-5p was the most competent one. After a median of 33 months of prospective follow-up, exosomal hsa-miR-486-5p also was an efficient predictive biomarker for progression or relapse of NFPAs. By protein-protein interaction network construction of hsa-miR-486-5p targeted genes, the core modules revealed a high possibility that exosomal hsa-miR-486-5p regulated tumor progression by epigenetic regulation of MAPK signaling pathways. In conclusion, exosomal hsa-miR-486-5p, hsa-miR-151a-5p, hsa-miR-652-3p_R+1, and hsa-miR-1180-3p are candidate biomarkers for diagnosis and screening of NFPAs. More importantly, prospective follow-up reveals that hsa-miR-486-5p can be regarded as a significant predictor for prognosis of NFPAs. Frontiers Media S.A. 2022-01-18 /pmc/articles/PMC8804500/ /pubmed/35118066 http://dx.doi.org/10.3389/fcell.2021.771354 Text en Copyright © 2022 Lyu, Li, Chen, Yu, Wang, Yin, Hu, Jiang, Ye and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Lyu, Liang
Li, Haiyan
Chen, Cheng
Yu, Yang
Wang, Li
Yin, Senlin
Hu, Yu
Jiang, Shu
Ye, Feng
Zhou, Peizhi
Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma
title Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma
title_full Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma
title_fullStr Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma
title_full_unstemmed Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma
title_short Exosomal miRNA Profiling is a Potential Screening Route for Non-Functional Pituitary Adenoma
title_sort exosomal mirna profiling is a potential screening route for non-functional pituitary adenoma
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8804500/
https://www.ncbi.nlm.nih.gov/pubmed/35118066
http://dx.doi.org/10.3389/fcell.2021.771354
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