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Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells

[Image: see text] The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu(3+) antenna, after self-assembling with this cation...

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Autores principales: Fueyo-González, Francisco, Espinar-Barranco, Laura, Herranz, Rosario, Alkorta, Ibon, Crovetto, Luis, Fribourg, Miguel, Paredes, Jose Manuel, Orte, Angel, González-Vera, Juan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805117/
https://www.ncbi.nlm.nih.gov/pubmed/35034437
http://dx.doi.org/10.1021/acssensors.1c02439
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author Fueyo-González, Francisco
Espinar-Barranco, Laura
Herranz, Rosario
Alkorta, Ibon
Crovetto, Luis
Fribourg, Miguel
Paredes, Jose Manuel
Orte, Angel
González-Vera, Juan A.
author_facet Fueyo-González, Francisco
Espinar-Barranco, Laura
Herranz, Rosario
Alkorta, Ibon
Crovetto, Luis
Fribourg, Miguel
Paredes, Jose Manuel
Orte, Angel
González-Vera, Juan A.
author_sort Fueyo-González, Francisco
collection PubMed
description [Image: see text] The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu(3+) antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4(+) T, CD8(+) T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu(3+) (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4(+) T, CD8(+) T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4(+) naïve T cells into regulatory T cells (T(REG)).
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spelling pubmed-88051172022-02-02 Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells Fueyo-González, Francisco Espinar-Barranco, Laura Herranz, Rosario Alkorta, Ibon Crovetto, Luis Fribourg, Miguel Paredes, Jose Manuel Orte, Angel González-Vera, Juan A. ACS Sens [Image: see text] The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu(3+) antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4(+) T, CD8(+) T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu(3+) (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4(+) T, CD8(+) T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4(+) naïve T cells into regulatory T cells (T(REG)). American Chemical Society 2022-01-15 2022-01-28 /pmc/articles/PMC8805117/ /pubmed/35034437 http://dx.doi.org/10.1021/acssensors.1c02439 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Fueyo-González, Francisco
Espinar-Barranco, Laura
Herranz, Rosario
Alkorta, Ibon
Crovetto, Luis
Fribourg, Miguel
Paredes, Jose Manuel
Orte, Angel
González-Vera, Juan A.
Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
title Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
title_full Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
title_fullStr Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
title_full_unstemmed Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
title_short Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
title_sort self-assembled lanthanide antenna glutathione sensor for the study of immune cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805117/
https://www.ncbi.nlm.nih.gov/pubmed/35034437
http://dx.doi.org/10.1021/acssensors.1c02439
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