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Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
[Image: see text] The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu(3+) antenna, after self-assembling with this cation...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805117/ https://www.ncbi.nlm.nih.gov/pubmed/35034437 http://dx.doi.org/10.1021/acssensors.1c02439 |
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author | Fueyo-González, Francisco Espinar-Barranco, Laura Herranz, Rosario Alkorta, Ibon Crovetto, Luis Fribourg, Miguel Paredes, Jose Manuel Orte, Angel González-Vera, Juan A. |
author_facet | Fueyo-González, Francisco Espinar-Barranco, Laura Herranz, Rosario Alkorta, Ibon Crovetto, Luis Fribourg, Miguel Paredes, Jose Manuel Orte, Angel González-Vera, Juan A. |
author_sort | Fueyo-González, Francisco |
collection | PubMed |
description | [Image: see text] The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu(3+) antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4(+) T, CD8(+) T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu(3+) (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4(+) T, CD8(+) T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4(+) naïve T cells into regulatory T cells (T(REG)). |
format | Online Article Text |
id | pubmed-8805117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-88051172022-02-02 Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells Fueyo-González, Francisco Espinar-Barranco, Laura Herranz, Rosario Alkorta, Ibon Crovetto, Luis Fribourg, Miguel Paredes, Jose Manuel Orte, Angel González-Vera, Juan A. ACS Sens [Image: see text] The small molecule 8-methoxy-2-oxo-1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu(3+) antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4(+) T, CD8(+) T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu(3+) (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4(+) T, CD8(+) T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4(+) naïve T cells into regulatory T cells (T(REG)). American Chemical Society 2022-01-15 2022-01-28 /pmc/articles/PMC8805117/ /pubmed/35034437 http://dx.doi.org/10.1021/acssensors.1c02439 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Fueyo-González, Francisco Espinar-Barranco, Laura Herranz, Rosario Alkorta, Ibon Crovetto, Luis Fribourg, Miguel Paredes, Jose Manuel Orte, Angel González-Vera, Juan A. Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells |
title | Self-Assembled Lanthanide Antenna Glutathione Sensor
for the Study of Immune Cells |
title_full | Self-Assembled Lanthanide Antenna Glutathione Sensor
for the Study of Immune Cells |
title_fullStr | Self-Assembled Lanthanide Antenna Glutathione Sensor
for the Study of Immune Cells |
title_full_unstemmed | Self-Assembled Lanthanide Antenna Glutathione Sensor
for the Study of Immune Cells |
title_short | Self-Assembled Lanthanide Antenna Glutathione Sensor
for the Study of Immune Cells |
title_sort | self-assembled lanthanide antenna glutathione sensor
for the study of immune cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805117/ https://www.ncbi.nlm.nih.gov/pubmed/35034437 http://dx.doi.org/10.1021/acssensors.1c02439 |
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