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Timing of Splenectomy after Acute Spinal Cord Injury

Spinal cord injury (SCI) is a devastating condition. Splenectomy may play a protective role in the development of SCI. However, little is known about whether the timing of splenectomy affects the outcome after SCI. Investigation into splenectomy after SCI would provide insight into how the timing ca...

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Autores principales: Wu, Feng, Li, Xiao-Hui, Gong, Min-Jie, An, Jia-Qi, Ding, Xiao-Yan, Huang, Sheng-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805191/
https://www.ncbi.nlm.nih.gov/pubmed/34996774
http://dx.doi.org/10.1523/ENEURO.0440-21.2021
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author Wu, Feng
Li, Xiao-Hui
Gong, Min-Jie
An, Jia-Qi
Ding, Xiao-Yan
Huang, Sheng-Li
author_facet Wu, Feng
Li, Xiao-Hui
Gong, Min-Jie
An, Jia-Qi
Ding, Xiao-Yan
Huang, Sheng-Li
author_sort Wu, Feng
collection PubMed
description Spinal cord injury (SCI) is a devastating condition. Splenectomy may play a protective role in the development of SCI. However, little is known about whether the timing of splenectomy affects the outcome after SCI. Investigation into splenectomy after SCI would provide insight into how the timing can be selected following SCI to improve neurologic outcomes. Rats were randomized into a sham group, a nonsplenectomized group (NonSPX), four splenectomized groups with the surgery performed immediately, 6 h, 12 h, and 24 h after SCI (SPX0, SPX6, SPX12, and SPX24, respectively). Rats were subjected to severe contusive SCI at the level of the third thoracic vertebra. At different time points following SCI, Basso, Beattie, and Bresnahan (BBB) score was used to assess the recovery of injury. The animals in each group were randomly selected for tissue collection at days 3, 14, and 28 after surgery. Then, immunohistochemistry of immunologic cells was performed and inflammatory mediators were determined. Our study showed that splenectomy within 6 h after SCI improved BBB scores as compared with splenectomy more than 12 h after SCI, and decrease the immune cell responses to SCI. Protein levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α were significantly elevated in nonsplenectomized group compared with sham group. No difference was observed in IL-10 at the lesion site between splenectomized and nonsplenectomized groups at 3 d post-SCI. The study demonstrates that splenectomy within 6 h after SCI would lessen the development of SCI and improve outcome.
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spelling pubmed-88051912022-02-01 Timing of Splenectomy after Acute Spinal Cord Injury Wu, Feng Li, Xiao-Hui Gong, Min-Jie An, Jia-Qi Ding, Xiao-Yan Huang, Sheng-Li eNeuro Research Article: New Research Spinal cord injury (SCI) is a devastating condition. Splenectomy may play a protective role in the development of SCI. However, little is known about whether the timing of splenectomy affects the outcome after SCI. Investigation into splenectomy after SCI would provide insight into how the timing can be selected following SCI to improve neurologic outcomes. Rats were randomized into a sham group, a nonsplenectomized group (NonSPX), four splenectomized groups with the surgery performed immediately, 6 h, 12 h, and 24 h after SCI (SPX0, SPX6, SPX12, and SPX24, respectively). Rats were subjected to severe contusive SCI at the level of the third thoracic vertebra. At different time points following SCI, Basso, Beattie, and Bresnahan (BBB) score was used to assess the recovery of injury. The animals in each group were randomly selected for tissue collection at days 3, 14, and 28 after surgery. Then, immunohistochemistry of immunologic cells was performed and inflammatory mediators were determined. Our study showed that splenectomy within 6 h after SCI improved BBB scores as compared with splenectomy more than 12 h after SCI, and decrease the immune cell responses to SCI. Protein levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α were significantly elevated in nonsplenectomized group compared with sham group. No difference was observed in IL-10 at the lesion site between splenectomized and nonsplenectomized groups at 3 d post-SCI. The study demonstrates that splenectomy within 6 h after SCI would lessen the development of SCI and improve outcome. Society for Neuroscience 2022-01-25 /pmc/articles/PMC8805191/ /pubmed/34996774 http://dx.doi.org/10.1523/ENEURO.0440-21.2021 Text en Copyright © 2022 Wu et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article: New Research
Wu, Feng
Li, Xiao-Hui
Gong, Min-Jie
An, Jia-Qi
Ding, Xiao-Yan
Huang, Sheng-Li
Timing of Splenectomy after Acute Spinal Cord Injury
title Timing of Splenectomy after Acute Spinal Cord Injury
title_full Timing of Splenectomy after Acute Spinal Cord Injury
title_fullStr Timing of Splenectomy after Acute Spinal Cord Injury
title_full_unstemmed Timing of Splenectomy after Acute Spinal Cord Injury
title_short Timing of Splenectomy after Acute Spinal Cord Injury
title_sort timing of splenectomy after acute spinal cord injury
topic Research Article: New Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8805191/
https://www.ncbi.nlm.nih.gov/pubmed/34996774
http://dx.doi.org/10.1523/ENEURO.0440-21.2021
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