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Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family
Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytopla...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806188/ https://www.ncbi.nlm.nih.gov/pubmed/35001872 http://dx.doi.org/10.7554/eLife.74589 |
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author | Ramanadane, Karthik Straub, Monique S Dutzler, Raimund Manatschal, Cristina |
author_facet | Ramanadane, Karthik Straub, Monique S Dutzler, Raimund Manatschal, Cristina |
author_sort | Ramanadane, Karthik |
collection | PubMed |
description | Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H(+) serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg(2+). The protein transports Mg(2+) and Mn(2+) but not Ca(2+) by a mechanism that is not coupled to H(+). Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell. |
format | Online Article Text |
id | pubmed-8806188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-88061882022-02-02 Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family Ramanadane, Karthik Straub, Monique S Dutzler, Raimund Manatschal, Cristina eLife Structural Biology and Molecular Biophysics Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H(+) serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg(2+). The protein transports Mg(2+) and Mn(2+) but not Ca(2+) by a mechanism that is not coupled to H(+). Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell. eLife Sciences Publications, Ltd 2022-01-10 /pmc/articles/PMC8806188/ /pubmed/35001872 http://dx.doi.org/10.7554/eLife.74589 Text en © 2022, Ramanadane et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Structural Biology and Molecular Biophysics Ramanadane, Karthik Straub, Monique S Dutzler, Raimund Manatschal, Cristina Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family |
title | Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family |
title_full | Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family |
title_fullStr | Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family |
title_full_unstemmed | Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family |
title_short | Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family |
title_sort | structural and functional properties of a magnesium transporter of the slc11/nramp family |
topic | Structural Biology and Molecular Biophysics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806188/ https://www.ncbi.nlm.nih.gov/pubmed/35001872 http://dx.doi.org/10.7554/eLife.74589 |
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