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Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448
In recent years, the incidence and mortality of myocardial infarction (MI) have been increasing throughout the world, threatening public health. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), play critical roles in the progression of MI. The present study...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806428/ https://www.ncbi.nlm.nih.gov/pubmed/34369259 http://dx.doi.org/10.1080/21655979.2021.1954135 |
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author | Nie, Shen Cui, Xiaoya Guo, Jinping Ma, Xiaohua Zhi, Haijun Li, Shilei Li, Yong |
author_facet | Nie, Shen Cui, Xiaoya Guo, Jinping Ma, Xiaohua Zhi, Haijun Li, Shilei Li, Yong |
author_sort | Nie, Shen |
collection | PubMed |
description | In recent years, the incidence and mortality of myocardial infarction (MI) have been increasing throughout the world, threatening public health. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), play critical roles in the progression of MI. The present study aimed to investigate the role of lncRNA AK006774 in the progression of myocardial infarction and find out novel therapeutic or diagnostic target of myocardial infarction. A mouse ischemia/reperfusion (I/R) model and 2,3,5-Triphenyte-trazoliumchloride (TTC) staining were performed to evaluate the effects of AK006774 on I/R injury in vivo. Hypoxia/reoxygenation (H/R) models using primary cardiomyocytes have been established. Flow cytometry and Terminal Deoxynucleotide Transferase dUTP Nick End Labeling (TUNEL) assays were performed to evaluate the effects of AK006774 on cardiomyocyte apoptosis. Luciferase and RNA pull-down assays were performed to verify the interaction between miR-448 and its targets. Western blotting and quantitative PCR were performed to determine protein and gene expression, respectively. We first found that AK006774 overexpression reduced I/R-induced infarct area and cardiomyocyte apoptosis in vivo. Accordingly, AK006774 inhibited apoptosis and oxidative stress in cardiomyocytes subjected to H/R treatment in vitro. Mechanistically, AK006774 modulated the expression of bcl-2 by sponging miR-448. Overexpression of miR-448 antagonized the effects of AK006774 on cardiomyocyte apoptosis. The AK006774/miR-448/bcl-2 signaling axis acts as a key regulator of I/R injury and may be a potential therapeutic or diagnostic target for the treatment of MI. |
format | Online Article Text |
id | pubmed-8806428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-88064282022-02-02 Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 Nie, Shen Cui, Xiaoya Guo, Jinping Ma, Xiaohua Zhi, Haijun Li, Shilei Li, Yong Bioengineered Research Paper In recent years, the incidence and mortality of myocardial infarction (MI) have been increasing throughout the world, threatening public health. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), play critical roles in the progression of MI. The present study aimed to investigate the role of lncRNA AK006774 in the progression of myocardial infarction and find out novel therapeutic or diagnostic target of myocardial infarction. A mouse ischemia/reperfusion (I/R) model and 2,3,5-Triphenyte-trazoliumchloride (TTC) staining were performed to evaluate the effects of AK006774 on I/R injury in vivo. Hypoxia/reoxygenation (H/R) models using primary cardiomyocytes have been established. Flow cytometry and Terminal Deoxynucleotide Transferase dUTP Nick End Labeling (TUNEL) assays were performed to evaluate the effects of AK006774 on cardiomyocyte apoptosis. Luciferase and RNA pull-down assays were performed to verify the interaction between miR-448 and its targets. Western blotting and quantitative PCR were performed to determine protein and gene expression, respectively. We first found that AK006774 overexpression reduced I/R-induced infarct area and cardiomyocyte apoptosis in vivo. Accordingly, AK006774 inhibited apoptosis and oxidative stress in cardiomyocytes subjected to H/R treatment in vitro. Mechanistically, AK006774 modulated the expression of bcl-2 by sponging miR-448. Overexpression of miR-448 antagonized the effects of AK006774 on cardiomyocyte apoptosis. The AK006774/miR-448/bcl-2 signaling axis acts as a key regulator of I/R injury and may be a potential therapeutic or diagnostic target for the treatment of MI. Taylor & Francis 2021-08-08 /pmc/articles/PMC8806428/ /pubmed/34369259 http://dx.doi.org/10.1080/21655979.2021.1954135 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Nie, Shen Cui, Xiaoya Guo, Jinping Ma, Xiaohua Zhi, Haijun Li, Shilei Li, Yong Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 |
title | Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 |
title_full | Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 |
title_fullStr | Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 |
title_full_unstemmed | Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 |
title_short | Long non-coding RNA AK006774 inhibits cardiac ischemia-reperfusion injury via sponging miR-448 |
title_sort | long non-coding rna ak006774 inhibits cardiac ischemia-reperfusion injury via sponging mir-448 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806428/ https://www.ncbi.nlm.nih.gov/pubmed/34369259 http://dx.doi.org/10.1080/21655979.2021.1954135 |
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