Cargando…

Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation

Oral squamous cell carcinoma (OSCC) is the most common tumor of the oral cavity. Studies have shown that exosomal miRNAs from cancer cells play an important role in mediating the cellular environment. The objective was to investigate the effect of OSCC-derived exosomes microRNA-221 (miR-221) in OSCC...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Shuqi, Zhang, Wu, Li, Xian, Wang, Junjie, Chen, Xufeng, Chen, Yu, Lai, Renfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806445/
https://www.ncbi.nlm.nih.gov/pubmed/34098850
http://dx.doi.org/10.1080/21655979.2021.1932222
_version_ 1784643449497059328
author He, Shuqi
Zhang, Wu
Li, Xian
Wang, Junjie
Chen, Xufeng
Chen, Yu
Lai, Renfa
author_facet He, Shuqi
Zhang, Wu
Li, Xian
Wang, Junjie
Chen, Xufeng
Chen, Yu
Lai, Renfa
author_sort He, Shuqi
collection PubMed
description Oral squamous cell carcinoma (OSCC) is the most common tumor of the oral cavity. Studies have shown that exosomal miRNAs from cancer cells play an important role in mediating the cellular environment. The objective was to investigate the effect of OSCC-derived exosomes microRNA-221 (miR-221) in OSCC. We used quantitative real-time PCR (qRT-PCR) and western blotting to determine PIK3R1 and miR-221 expressions in OSCC tissue or peripheral blood serum. Exosomes of OSCC cell line CAL27 were extracted and characterized. Exosomal miR-221 expression was detected by qRT-PCR. Dual-luciferase was performed to validate the targeted regulatory relationship of miR-221 on PIK3R1. Transwell and tube formation assay were applied to detect the effect of OSCC-derived exosomal miR-221 on HUVEC migration and angiogenesis. qRT-PCR confirmed that PIK3R1 expression was downregulated in OSCC tissue and cell line, while miR-221 expression was upregulated. miR-221 expression in OSCC cell line-derived exosome elevated. miR-221 could target and negatively regulate PIK3R1 expression. In addition, OSCC-derived miR-221 could promote HUVEC migration and angiogenesis. In conclusion, OSCC-derived exosomal miR-221 could target and negatively regulate PIK3R1 expression, as well as promote vascular endothelial cell migration and angiogenesis.
format Online
Article
Text
id pubmed-8806445
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-88064452022-02-02 Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation He, Shuqi Zhang, Wu Li, Xian Wang, Junjie Chen, Xufeng Chen, Yu Lai, Renfa Bioengineered Research Paper Oral squamous cell carcinoma (OSCC) is the most common tumor of the oral cavity. Studies have shown that exosomal miRNAs from cancer cells play an important role in mediating the cellular environment. The objective was to investigate the effect of OSCC-derived exosomes microRNA-221 (miR-221) in OSCC. We used quantitative real-time PCR (qRT-PCR) and western blotting to determine PIK3R1 and miR-221 expressions in OSCC tissue or peripheral blood serum. Exosomes of OSCC cell line CAL27 were extracted and characterized. Exosomal miR-221 expression was detected by qRT-PCR. Dual-luciferase was performed to validate the targeted regulatory relationship of miR-221 on PIK3R1. Transwell and tube formation assay were applied to detect the effect of OSCC-derived exosomal miR-221 on HUVEC migration and angiogenesis. qRT-PCR confirmed that PIK3R1 expression was downregulated in OSCC tissue and cell line, while miR-221 expression was upregulated. miR-221 expression in OSCC cell line-derived exosome elevated. miR-221 could target and negatively regulate PIK3R1 expression. In addition, OSCC-derived miR-221 could promote HUVEC migration and angiogenesis. In conclusion, OSCC-derived exosomal miR-221 could target and negatively regulate PIK3R1 expression, as well as promote vascular endothelial cell migration and angiogenesis. Taylor & Francis 2021-06-08 /pmc/articles/PMC8806445/ /pubmed/34098850 http://dx.doi.org/10.1080/21655979.2021.1932222 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
He, Shuqi
Zhang, Wu
Li, Xian
Wang, Junjie
Chen, Xufeng
Chen, Yu
Lai, Renfa
Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation
title Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation
title_full Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation
title_fullStr Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation
title_full_unstemmed Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation
title_short Oral squamous cell carcinoma (OSCC)-derived exosomal MiR-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) to promote human umbilical vein endothelial cells migration and tube formation
title_sort oral squamous cell carcinoma (oscc)-derived exosomal mir-221 targets and regulates phosphoinositide-3-kinase regulatory subunit 1 (pik3r1) to promote human umbilical vein endothelial cells migration and tube formation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806445/
https://www.ncbi.nlm.nih.gov/pubmed/34098850
http://dx.doi.org/10.1080/21655979.2021.1932222
work_keys_str_mv AT heshuqi oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation
AT zhangwu oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation
AT lixian oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation
AT wangjunjie oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation
AT chenxufeng oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation
AT chenyu oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation
AT lairenfa oralsquamouscellcarcinomaosccderivedexosomalmir221targetsandregulatesphosphoinositide3kinaseregulatorysubunit1pik3r1topromotehumanumbilicalveinendothelialcellsmigrationandtubeformation