Cargando…

Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer

The X-ray repair cross-complementing gene (XRCC) family participates in DNA damage repair and its dysregulation is associated with the development and progression of a variety of cancers. However, XRCCs have not been systematically studied in non-small cell lung cancer (NSCLC). Using The Cancer Geno...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Yongfei, Gao, Zhaojia, Li, Xinwei, Wei, Shuzhang, Yuan, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806547/
https://www.ncbi.nlm.nih.gov/pubmed/34486486
http://dx.doi.org/10.1080/21655979.2021.1964193
_version_ 1784643474548588544
author Fan, Yongfei
Gao, Zhaojia
Li, Xinwei
Wei, Shuzhang
Yuan, Kai
author_facet Fan, Yongfei
Gao, Zhaojia
Li, Xinwei
Wei, Shuzhang
Yuan, Kai
author_sort Fan, Yongfei
collection PubMed
description The X-ray repair cross-complementing gene (XRCC) family participates in DNA damage repair and its dysregulation is associated with the development and progression of a variety of cancers. However, XRCCs have not been systematically studied in non-small cell lung cancer (NSCLC). Using The Cancer Genome Atlas (TCGA) and Oncomine databases, we compared the expression levels of XRCCs between NSCLC and normal tissues and performed survival analysis using the data from TCGA. The correlations of XRCCs with the clinical parameters were then analyzed using UCSC Xena. Genetic alterations in XRCCs in NSCLC and their effects on the prognosis of patients were presented using cBioPortal. SurvivalMeth was used to explore the differentially methylated sites associated with NSCLC and their effect on prognosis. Next, the immunological correlations of XRCCs expression level were analyzed using TIMER 2.0. Finally, GeneMANIA was used to visualize and analyze the functionally relevant genes, while Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used for functional and pathway enrichment analyses of prognostic genes. Our results revealed that XRCCs were overexpressed in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Univariate and multivariate Cox analyses showed that XRCC4/5/6 were independent risk factors for LUAD. Additionally, genetic alterations, methylation, and immune cell infiltration demonstrated an association between XRCC4/5/6 and poor prognosis in LUAD. Finally, the KEGG-enriched and non-homologous end-joining (NHEJ) pathways were shown to be associated with XRCC4/5/6. In conclusion, our study demonstrated that XRCC4/5/6 could be used as diagnostic and prognostic biomarkers for LUAD.
format Online
Article
Text
id pubmed-8806547
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-88065472022-02-02 Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer Fan, Yongfei Gao, Zhaojia Li, Xinwei Wei, Shuzhang Yuan, Kai Bioengineered Research Paper The X-ray repair cross-complementing gene (XRCC) family participates in DNA damage repair and its dysregulation is associated with the development and progression of a variety of cancers. However, XRCCs have not been systematically studied in non-small cell lung cancer (NSCLC). Using The Cancer Genome Atlas (TCGA) and Oncomine databases, we compared the expression levels of XRCCs between NSCLC and normal tissues and performed survival analysis using the data from TCGA. The correlations of XRCCs with the clinical parameters were then analyzed using UCSC Xena. Genetic alterations in XRCCs in NSCLC and their effects on the prognosis of patients were presented using cBioPortal. SurvivalMeth was used to explore the differentially methylated sites associated with NSCLC and their effect on prognosis. Next, the immunological correlations of XRCCs expression level were analyzed using TIMER 2.0. Finally, GeneMANIA was used to visualize and analyze the functionally relevant genes, while Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used for functional and pathway enrichment analyses of prognostic genes. Our results revealed that XRCCs were overexpressed in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Univariate and multivariate Cox analyses showed that XRCC4/5/6 were independent risk factors for LUAD. Additionally, genetic alterations, methylation, and immune cell infiltration demonstrated an association between XRCC4/5/6 and poor prognosis in LUAD. Finally, the KEGG-enriched and non-homologous end-joining (NHEJ) pathways were shown to be associated with XRCC4/5/6. In conclusion, our study demonstrated that XRCC4/5/6 could be used as diagnostic and prognostic biomarkers for LUAD. Taylor & Francis 2021-09-05 /pmc/articles/PMC8806547/ /pubmed/34486486 http://dx.doi.org/10.1080/21655979.2021.1964193 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Fan, Yongfei
Gao, Zhaojia
Li, Xinwei
Wei, Shuzhang
Yuan, Kai
Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
title Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
title_full Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
title_fullStr Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
title_full_unstemmed Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
title_short Gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
title_sort gene expression and prognosis of x-ray repair cross-complementing family members in non-small cell lung cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806547/
https://www.ncbi.nlm.nih.gov/pubmed/34486486
http://dx.doi.org/10.1080/21655979.2021.1964193
work_keys_str_mv AT fanyongfei geneexpressionandprognosisofxrayrepaircrosscomplementingfamilymembersinnonsmallcelllungcancer
AT gaozhaojia geneexpressionandprognosisofxrayrepaircrosscomplementingfamilymembersinnonsmallcelllungcancer
AT lixinwei geneexpressionandprognosisofxrayrepaircrosscomplementingfamilymembersinnonsmallcelllungcancer
AT weishuzhang geneexpressionandprognosisofxrayrepaircrosscomplementingfamilymembersinnonsmallcelllungcancer
AT yuankai geneexpressionandprognosisofxrayrepaircrosscomplementingfamilymembersinnonsmallcelllungcancer