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Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma
Epithelial–mesenchymal transition (EMT)-related long non-coding RNAs (lncRNAs) may be exploited as potential therapeutic targets in gliomas. However, the prognostic value of EMT-related lncRNAs in gliomas is unclear. We obtained lncRNAs from The Cancer Genome Atlas and constructed EMT-related lncRNA...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806607/ https://www.ncbi.nlm.nih.gov/pubmed/34288803 http://dx.doi.org/10.1080/21655979.2021.1951927 |
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author | Tao, Chuming Luo, Haitao Chen, Luyue Li, Jingying Zhu, Xingen Huang, Kai |
author_facet | Tao, Chuming Luo, Haitao Chen, Luyue Li, Jingying Zhu, Xingen Huang, Kai |
author_sort | Tao, Chuming |
collection | PubMed |
description | Epithelial–mesenchymal transition (EMT)-related long non-coding RNAs (lncRNAs) may be exploited as potential therapeutic targets in gliomas. However, the prognostic value of EMT-related lncRNAs in gliomas is unclear. We obtained lncRNAs from The Cancer Genome Atlas and constructed EMT-related lncRNA co-expression networks to identify EMT-related lncRNAs. The Chinese Glioma Genome Atlas (CGGA) was used for validation. Gene set enrichment and principal component analyses were used for functional annotation. The EMT–lncRNA co-expression networks were constructed. A real-time quantitative polymerase chain reaction assay was performed to validate the bioinformatics results. A nine-EMT-related lncRNAs (HAR1A, LINC00641, LINC00900, MIR210HG, MIR22HG, PVT1, SLC25A21-AS1, SNAI3-AS1, and SNHG18) signature was identified in patients with glioma. Patients in the low-risk group had a longer overall survival (OS) than those in the high-risk group (P < 0.0001). Additionally, patients in the high-risk group showed no deletion of chromosomal arms 1p and/or 19q, isocitrate dehydrogenase wild type, and higher World Health Organization grade. Moreover, the signature was identified as an independent factor and was significantly associated with OS (P = 0.041, hazard ratio = 1.806). These findings were further validated using the CGGA dataset. The low- and high-risk groups showed different EMT statuses based on principal component analysis. To study the regulatory function of lncRNAs, a lncRNA-mediated ceRNA network was constructed, which showed that complex interactions of lncRNA–miRNA–mRNA may be a potential cause of EMT progression in gliomas. This study showed that the nine-EMT-related lncRNA signature has a prognostic value in gliomas. |
format | Online Article Text |
id | pubmed-8806607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-88066072022-02-02 Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma Tao, Chuming Luo, Haitao Chen, Luyue Li, Jingying Zhu, Xingen Huang, Kai Bioengineered Research Paper Epithelial–mesenchymal transition (EMT)-related long non-coding RNAs (lncRNAs) may be exploited as potential therapeutic targets in gliomas. However, the prognostic value of EMT-related lncRNAs in gliomas is unclear. We obtained lncRNAs from The Cancer Genome Atlas and constructed EMT-related lncRNA co-expression networks to identify EMT-related lncRNAs. The Chinese Glioma Genome Atlas (CGGA) was used for validation. Gene set enrichment and principal component analyses were used for functional annotation. The EMT–lncRNA co-expression networks were constructed. A real-time quantitative polymerase chain reaction assay was performed to validate the bioinformatics results. A nine-EMT-related lncRNAs (HAR1A, LINC00641, LINC00900, MIR210HG, MIR22HG, PVT1, SLC25A21-AS1, SNAI3-AS1, and SNHG18) signature was identified in patients with glioma. Patients in the low-risk group had a longer overall survival (OS) than those in the high-risk group (P < 0.0001). Additionally, patients in the high-risk group showed no deletion of chromosomal arms 1p and/or 19q, isocitrate dehydrogenase wild type, and higher World Health Organization grade. Moreover, the signature was identified as an independent factor and was significantly associated with OS (P = 0.041, hazard ratio = 1.806). These findings were further validated using the CGGA dataset. The low- and high-risk groups showed different EMT statuses based on principal component analysis. To study the regulatory function of lncRNAs, a lncRNA-mediated ceRNA network was constructed, which showed that complex interactions of lncRNA–miRNA–mRNA may be a potential cause of EMT progression in gliomas. This study showed that the nine-EMT-related lncRNA signature has a prognostic value in gliomas. Taylor & Francis 2021-07-21 /pmc/articles/PMC8806607/ /pubmed/34288803 http://dx.doi.org/10.1080/21655979.2021.1951927 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Tao, Chuming Luo, Haitao Chen, Luyue Li, Jingying Zhu, Xingen Huang, Kai Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma |
title | Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma |
title_full | Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma |
title_fullStr | Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma |
title_full_unstemmed | Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma |
title_short | Identification of an epithelial-mesenchymal transition related long non-coding RNA (LncRNA) signature in Glioma |
title_sort | identification of an epithelial-mesenchymal transition related long non-coding rna (lncrna) signature in glioma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806607/ https://www.ncbi.nlm.nih.gov/pubmed/34288803 http://dx.doi.org/10.1080/21655979.2021.1951927 |
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