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Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis

Although the mechanism of osteoarthritis (OA) has been widely studied and the use of quercetin for OA therapy is well documented, the relevant characteristics of the microbiome and metabolism remain unclear. This study reports changes in the gut microbiota and metabolism during quercetin therapy for...

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Autores principales: Lan, Haifeng, Hong, Wei, Qian, Dongyang, Peng, Fang, Li, Haiqing, Liang, Chunxiao, Du, Min, Gu, Jinlan, Mai, Junxuan, Bai, Bo, Peng, Gongyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806632/
https://www.ncbi.nlm.nih.gov/pubmed/34486477
http://dx.doi.org/10.1080/21655979.2021.1969194
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author Lan, Haifeng
Hong, Wei
Qian, Dongyang
Peng, Fang
Li, Haiqing
Liang, Chunxiao
Du, Min
Gu, Jinlan
Mai, Junxuan
Bai, Bo
Peng, Gongyong
author_facet Lan, Haifeng
Hong, Wei
Qian, Dongyang
Peng, Fang
Li, Haiqing
Liang, Chunxiao
Du, Min
Gu, Jinlan
Mai, Junxuan
Bai, Bo
Peng, Gongyong
author_sort Lan, Haifeng
collection PubMed
description Although the mechanism of osteoarthritis (OA) has been widely studied and the use of quercetin for OA therapy is well documented, the relevant characteristics of the microbiome and metabolism remain unclear. This study reports changes in the gut microbiota and metabolism during quercetin therapy for OA in a rat model and provides an integrative analysis of the biomechanism. In this study, the rats were categorized into 3 different groups: the OA model, quercetin treatment, and control groups. The OA rats was conducted using a monoiodoacetate (MIA) injection protocol. The rats in the quercetin group received daily intragastric administration of quercetin from day 1 to day 28. Stool samples were collected, and DNA was extracted. We used an integrated approach that combined the sequencing of whole 16S rRNA, short-chain fatty acid (SCFA) measurements and metabolomics analysis by mass spectrometry (MS) to characterize the functional impact of quercetin on the gut microbiota and metabolism in a rat model of OA. The use of quercetin partially abrogated intestinal flora disorder and reversed fecal metabolite abnormalities. Compared with the control rats, the OA rats showed differences at both the class level (Clostridia, Bacteroidia, and Bacilli) and the genus level (Lactobacillus and unidentified Ruminococcaceae). Acetic acid, propionic acid and 24 metabolites were significantly altered among the three groups. However, the changes were significantly abrogated in quercetin-treated OA rats. Consequently, this study provided important evidence regarding perturbations of the gut microbiome and the function of these changes in a potential new mechanism of quercetin treatment.
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spelling pubmed-88066322022-02-02 Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis Lan, Haifeng Hong, Wei Qian, Dongyang Peng, Fang Li, Haiqing Liang, Chunxiao Du, Min Gu, Jinlan Mai, Junxuan Bai, Bo Peng, Gongyong Bioengineered Research Paper Although the mechanism of osteoarthritis (OA) has been widely studied and the use of quercetin for OA therapy is well documented, the relevant characteristics of the microbiome and metabolism remain unclear. This study reports changes in the gut microbiota and metabolism during quercetin therapy for OA in a rat model and provides an integrative analysis of the biomechanism. In this study, the rats were categorized into 3 different groups: the OA model, quercetin treatment, and control groups. The OA rats was conducted using a monoiodoacetate (MIA) injection protocol. The rats in the quercetin group received daily intragastric administration of quercetin from day 1 to day 28. Stool samples were collected, and DNA was extracted. We used an integrated approach that combined the sequencing of whole 16S rRNA, short-chain fatty acid (SCFA) measurements and metabolomics analysis by mass spectrometry (MS) to characterize the functional impact of quercetin on the gut microbiota and metabolism in a rat model of OA. The use of quercetin partially abrogated intestinal flora disorder and reversed fecal metabolite abnormalities. Compared with the control rats, the OA rats showed differences at both the class level (Clostridia, Bacteroidia, and Bacilli) and the genus level (Lactobacillus and unidentified Ruminococcaceae). Acetic acid, propionic acid and 24 metabolites were significantly altered among the three groups. However, the changes were significantly abrogated in quercetin-treated OA rats. Consequently, this study provided important evidence regarding perturbations of the gut microbiome and the function of these changes in a potential new mechanism of quercetin treatment. Taylor & Francis 2021-09-05 /pmc/articles/PMC8806632/ /pubmed/34486477 http://dx.doi.org/10.1080/21655979.2021.1969194 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Lan, Haifeng
Hong, Wei
Qian, Dongyang
Peng, Fang
Li, Haiqing
Liang, Chunxiao
Du, Min
Gu, Jinlan
Mai, Junxuan
Bai, Bo
Peng, Gongyong
Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
title Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
title_full Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
title_fullStr Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
title_full_unstemmed Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
title_short Quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
title_sort quercetin modulates the gut microbiota as well as the metabolome in a rat model of osteoarthritis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806632/
https://www.ncbi.nlm.nih.gov/pubmed/34486477
http://dx.doi.org/10.1080/21655979.2021.1969194
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