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Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer

Endometrial cancer (EC) is the most common gynecological malignancy. Recent studies have uncovered miRNA acted a striking role in predicting the prognosis of multiple tumors. Over 500 EC samples were selected from the Cancer Genome Atlas (TCGA) database. Univariate, LASSO and multivariate Cox regres...

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Autores principales: Lu, Jing, Liang, Jianqiang, Xu, Mengting, Wu, Zhipeng, Cheng, Wenjun, Wu, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806668/
https://www.ncbi.nlm.nih.gov/pubmed/34338136
http://dx.doi.org/10.1080/21655979.2021.1952051
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author Lu, Jing
Liang, Jianqiang
Xu, Mengting
Wu, Zhipeng
Cheng, Wenjun
Wu, Jie
author_facet Lu, Jing
Liang, Jianqiang
Xu, Mengting
Wu, Zhipeng
Cheng, Wenjun
Wu, Jie
author_sort Lu, Jing
collection PubMed
description Endometrial cancer (EC) is the most common gynecological malignancy. Recent studies have uncovered miRNA acted a striking role in predicting the prognosis of multiple tumors. Over 500 EC samples were selected from the Cancer Genome Atlas (TCGA) database. Univariate, LASSO and multivariate Cox regression analysis were employed to screen out the prognosis-involved miRNAs. Kaplan-Meier (K-M) and time-dependent receiver operation characteristic (ROC) curves were conducted to reveal survival analysis and assess the accuracy of the signature. The independence of the model was verified via univariate and multivariate Cox regression analysis. Besides, qRT-PCR was conducted to testified the expression of 11 miRNAs in 16 paired tissues. A total of 514 specimens were randomly divided into the training set and the testing set, then an 11 miRNAs-based signature were determined which divided the patients into high-risk group and low-risk group. The survival was markedly different and the ROC curve exhibited a precise prediction. Meanwhile, the univariate and multivariate Cox regression analysis verified the miRNAs-based model was an independent indicator of EC. Moreove, the prediction ability of this model with clinicopathological features was more efficient. Finally, functional enrichment analysis demonstrated these miRNAs were associated with the occurrence and progression of cancer. Additionally, hsa-mir-216b, hsa-mir-363, hsa-mir-940 and hsa-mir-1301 were highly expressed in EC tissues in contrast to normal tissues through qRT-PCR. Importantly, the eleven-miRNA signature was full of robust ability to predict the prognosis of EC.
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spelling pubmed-88066682022-02-02 Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer Lu, Jing Liang, Jianqiang Xu, Mengting Wu, Zhipeng Cheng, Wenjun Wu, Jie Bioengineered Research Paper Endometrial cancer (EC) is the most common gynecological malignancy. Recent studies have uncovered miRNA acted a striking role in predicting the prognosis of multiple tumors. Over 500 EC samples were selected from the Cancer Genome Atlas (TCGA) database. Univariate, LASSO and multivariate Cox regression analysis were employed to screen out the prognosis-involved miRNAs. Kaplan-Meier (K-M) and time-dependent receiver operation characteristic (ROC) curves were conducted to reveal survival analysis and assess the accuracy of the signature. The independence of the model was verified via univariate and multivariate Cox regression analysis. Besides, qRT-PCR was conducted to testified the expression of 11 miRNAs in 16 paired tissues. A total of 514 specimens were randomly divided into the training set and the testing set, then an 11 miRNAs-based signature were determined which divided the patients into high-risk group and low-risk group. The survival was markedly different and the ROC curve exhibited a precise prediction. Meanwhile, the univariate and multivariate Cox regression analysis verified the miRNAs-based model was an independent indicator of EC. Moreove, the prediction ability of this model with clinicopathological features was more efficient. Finally, functional enrichment analysis demonstrated these miRNAs were associated with the occurrence and progression of cancer. Additionally, hsa-mir-216b, hsa-mir-363, hsa-mir-940 and hsa-mir-1301 were highly expressed in EC tissues in contrast to normal tissues through qRT-PCR. Importantly, the eleven-miRNA signature was full of robust ability to predict the prognosis of EC. Taylor & Francis 2021-08-01 /pmc/articles/PMC8806668/ /pubmed/34338136 http://dx.doi.org/10.1080/21655979.2021.1952051 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Lu, Jing
Liang, Jianqiang
Xu, Mengting
Wu, Zhipeng
Cheng, Wenjun
Wu, Jie
Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer
title Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer
title_full Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer
title_fullStr Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer
title_full_unstemmed Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer
title_short Identification of an eleven-miRNA signature to predict the prognosis of endometrial cancer
title_sort identification of an eleven-mirna signature to predict the prognosis of endometrial cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806668/
https://www.ncbi.nlm.nih.gov/pubmed/34338136
http://dx.doi.org/10.1080/21655979.2021.1952051
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