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MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1)
The microRNA miR-130a-3p (miR-130a-3p) has anti-tumor activity against numerous cancer types. Further, miR-130a-3p may target Wnt signaling, which is a critical pathway regulating tumorigenesis. Functions of miR-130a-3p in colorectal cancer (CRC) and contributions of Wnt1 pathway modulation, however...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806712/ https://www.ncbi.nlm.nih.gov/pubmed/34657551 http://dx.doi.org/10.1080/21655979.2021.1977556 |
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author | Song, Guang-Lin Xiao, Ming Wan, Xiao-Ya Deng, Jun Ling, Jun-Da Tian, Ying-Guo Li, Min Yin, Jie Zheng, Ren-Ying Tang, Yi Liu, Gui-Yuan |
author_facet | Song, Guang-Lin Xiao, Ming Wan, Xiao-Ya Deng, Jun Ling, Jun-Da Tian, Ying-Guo Li, Min Yin, Jie Zheng, Ren-Ying Tang, Yi Liu, Gui-Yuan |
author_sort | Song, Guang-Lin |
collection | PubMed |
description | The microRNA miR-130a-3p (miR-130a-3p) has anti-tumor activity against numerous cancer types. Further, miR-130a-3p may target Wnt signaling, which is a critical pathway regulating tumorigenesis. Functions of miR-130a-3p in colorectal cancer (CRC) and contributions of Wnt1 pathway modulation, however, have not been examined, hence the exploration on these two aspects. In this study, in comparison with normal controls, both CRC tissue and multiple CRC cell lines showed downregulated miR-130a-3p. MiR-130a-3p overexpression contributed to a decrease in CRC cell proliferation. Additionally, its overexpression also caused reduced expression of WNT Family Member 1 (WNT1) and downstream WNT pathway factors c-myc and cyclin D1. Dual-luciferase assay revealed WNT1 as a direct target of miR-130a-3p, and further the inhibitory effect of miR-130a-3p on c-myc and cyclin D1 was proved to be reversed by overexpressed WNT1. Collectively, miR-130a-3p inhibits CRC growth by directly targeting WNT1, and miR-130a-3p and WNT1 pathway-associated factors are defined as potential targets for CRC treatment. |
format | Online Article Text |
id | pubmed-8806712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-88067122022-02-02 MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) Song, Guang-Lin Xiao, Ming Wan, Xiao-Ya Deng, Jun Ling, Jun-Da Tian, Ying-Guo Li, Min Yin, Jie Zheng, Ren-Ying Tang, Yi Liu, Gui-Yuan Bioengineered Research Paper The microRNA miR-130a-3p (miR-130a-3p) has anti-tumor activity against numerous cancer types. Further, miR-130a-3p may target Wnt signaling, which is a critical pathway regulating tumorigenesis. Functions of miR-130a-3p in colorectal cancer (CRC) and contributions of Wnt1 pathway modulation, however, have not been examined, hence the exploration on these two aspects. In this study, in comparison with normal controls, both CRC tissue and multiple CRC cell lines showed downregulated miR-130a-3p. MiR-130a-3p overexpression contributed to a decrease in CRC cell proliferation. Additionally, its overexpression also caused reduced expression of WNT Family Member 1 (WNT1) and downstream WNT pathway factors c-myc and cyclin D1. Dual-luciferase assay revealed WNT1 as a direct target of miR-130a-3p, and further the inhibitory effect of miR-130a-3p on c-myc and cyclin D1 was proved to be reversed by overexpressed WNT1. Collectively, miR-130a-3p inhibits CRC growth by directly targeting WNT1, and miR-130a-3p and WNT1 pathway-associated factors are defined as potential targets for CRC treatment. Taylor & Francis 2021-10-18 /pmc/articles/PMC8806712/ /pubmed/34657551 http://dx.doi.org/10.1080/21655979.2021.1977556 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Song, Guang-Lin Xiao, Ming Wan, Xiao-Ya Deng, Jun Ling, Jun-Da Tian, Ying-Guo Li, Min Yin, Jie Zheng, Ren-Ying Tang, Yi Liu, Gui-Yuan MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) |
title | MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) |
title_full | MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) |
title_fullStr | MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) |
title_full_unstemmed | MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) |
title_short | MiR-130a-3p suppresses colorectal cancer growth by targeting Wnt Family Member 1 (WNT1) |
title_sort | mir-130a-3p suppresses colorectal cancer growth by targeting wnt family member 1 (wnt1) |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806712/ https://www.ncbi.nlm.nih.gov/pubmed/34657551 http://dx.doi.org/10.1080/21655979.2021.1977556 |
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