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A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers

Beta-actin (ACTB), a highly conserved cytoskeleton structural protein, has been regarded as a common housekeep gene and used as a reference gene for years. However, accumulating evidence indicates that ACTB is abnormally expressed in multiple cancers and hence changes the cytoskeleton to affect the...

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Autores principales: Gu, Yuxi, Tang, Shouyi, Wang, Zhen, Cai, Luyao, Lian, Haosen, Shen, Yingqiang, Zhou, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806805/
https://www.ncbi.nlm.nih.gov/pubmed/34486492
http://dx.doi.org/10.1080/21655979.2021.1973220
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author Gu, Yuxi
Tang, Shouyi
Wang, Zhen
Cai, Luyao
Lian, Haosen
Shen, Yingqiang
Zhou, Yu
author_facet Gu, Yuxi
Tang, Shouyi
Wang, Zhen
Cai, Luyao
Lian, Haosen
Shen, Yingqiang
Zhou, Yu
author_sort Gu, Yuxi
collection PubMed
description Beta-actin (ACTB), a highly conserved cytoskeleton structural protein, has been regarded as a common housekeep gene and used as a reference gene for years. However, accumulating evidence indicates that ACTB is abnormally expressed in multiple cancers and hence changes the cytoskeleton to affect the invasiveness and metastasis of tumors. This study aimed to investigate the function and clinical significance of ACTB in pan-cancer. The role of ACTB for prognosis and immune regulation across 33 tumors was explored based on the datasets of gene expression omnibus and the cancer genome atlas. Differential expression of ACTB was found between cancer and adjacent normal tissues, and significant associations was found between ACTB expression and prognosis of tumor patients. In most cancers, ACTB expression was associated with immune cells infiltration, immune checkpoints and other immune modulators. Relevance between ACTB and metastasis and invasion was identified in various types of cancers by CancerSEA. Moreover, focal adhesion and actin regulation-associated pathways were included in the functional mechanisms of ACTB. The expression of ACTB was verified by quantitative real-time polymerase chain reaction. Knockdown of ACTB inhibited head and neck squamous carcinoma cell migration and invasion by NF-κB and Wnt/β-catenin pathways. Our first pan-cancer study of ACTB offers insight into the prognostic and immunological roles of ACTB across different tumors, indicating ACTB may be a potential biomarker for poor prognosis and immune infiltration in cancers, and the role of ACTB as a reference gene in cancers was challenged.
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spelling pubmed-88068052022-02-02 A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers Gu, Yuxi Tang, Shouyi Wang, Zhen Cai, Luyao Lian, Haosen Shen, Yingqiang Zhou, Yu Bioengineered Research Paper Beta-actin (ACTB), a highly conserved cytoskeleton structural protein, has been regarded as a common housekeep gene and used as a reference gene for years. However, accumulating evidence indicates that ACTB is abnormally expressed in multiple cancers and hence changes the cytoskeleton to affect the invasiveness and metastasis of tumors. This study aimed to investigate the function and clinical significance of ACTB in pan-cancer. The role of ACTB for prognosis and immune regulation across 33 tumors was explored based on the datasets of gene expression omnibus and the cancer genome atlas. Differential expression of ACTB was found between cancer and adjacent normal tissues, and significant associations was found between ACTB expression and prognosis of tumor patients. In most cancers, ACTB expression was associated with immune cells infiltration, immune checkpoints and other immune modulators. Relevance between ACTB and metastasis and invasion was identified in various types of cancers by CancerSEA. Moreover, focal adhesion and actin regulation-associated pathways were included in the functional mechanisms of ACTB. The expression of ACTB was verified by quantitative real-time polymerase chain reaction. Knockdown of ACTB inhibited head and neck squamous carcinoma cell migration and invasion by NF-κB and Wnt/β-catenin pathways. Our first pan-cancer study of ACTB offers insight into the prognostic and immunological roles of ACTB across different tumors, indicating ACTB may be a potential biomarker for poor prognosis and immune infiltration in cancers, and the role of ACTB as a reference gene in cancers was challenged. Taylor & Francis 2021-09-04 /pmc/articles/PMC8806805/ /pubmed/34486492 http://dx.doi.org/10.1080/21655979.2021.1973220 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Gu, Yuxi
Tang, Shouyi
Wang, Zhen
Cai, Luyao
Lian, Haosen
Shen, Yingqiang
Zhou, Yu
A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers
title A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers
title_full A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers
title_fullStr A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers
title_full_unstemmed A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers
title_short A pan-cancer analysis of the prognostic and immunological role of β-actin (ACTB) in human cancers
title_sort pan-cancer analysis of the prognostic and immunological role of β-actin (actb) in human cancers
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806805/
https://www.ncbi.nlm.nih.gov/pubmed/34486492
http://dx.doi.org/10.1080/21655979.2021.1973220
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