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The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation

To explore mechanism of intestinal flora dysregulation promoting constipation, 60 specific pathogen-free (SPF) mice were used as research objects and were treated with constipation population fecal fluid gavage and distilled water gavage. Then, relationship between intestinal dysregulation and const...

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Autores principales: Fu, Ruibiao, Li, Zhongpeng, Zhou, Rui, Li, Chaoyang, Shao, Shuai, Li, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806846/
https://www.ncbi.nlm.nih.gov/pubmed/34517785
http://dx.doi.org/10.1080/21655979.2021.1973356
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author Fu, Ruibiao
Li, Zhongpeng
Zhou, Rui
Li, Chaoyang
Shao, Shuai
Li, Jin
author_facet Fu, Ruibiao
Li, Zhongpeng
Zhou, Rui
Li, Chaoyang
Shao, Shuai
Li, Jin
author_sort Fu, Ruibiao
collection PubMed
description To explore mechanism of intestinal flora dysregulation promoting constipation, 60 specific pathogen-free (SPF) mice were used as research objects and were treated with constipation population fecal fluid gavage and distilled water gavage. Then, relationship between intestinal dysregulation and constipation in mice with biofilm-mediated intestinal flora was investigated in vitro. The results showed that recombinant serotonin transporter (SERT) messenger ribonucleic acid (mRNA) level of the constipation population fecal fluid gavage group and the relative expression level of SERT mRNA were 1.61 ± 0.08 and 1.49 ± 0.06, which were higher markedly than those of distilled water group (P < 0.05). The level of 5-hydroxytryptamine (5-HT) in colonic tissue of the constipation population fecal fluid gavage group was 145.36 ± 14.12 ng/mL, and the expression level of 5-HT on the surface of epithelial cells of biofilm-positive colonic tissue was 20.11 ± 2.03, which were significantly lower than those of the distilled water group, with statistical significance (P < 0.05). Besides, the microbial sequencing of fecal flora indicated that The Akk and bacteroidetes ofconstipation population fecal fluid gavage group were higher hugely than those of distilled water group (P < 0.05).In conclusion, after the occurrence of constipation, the diversity of intestinal microflora decreased, and the probiotics reduced. Iintestinal microflora dysregulation would lead to increase of SERT expression level in defecation function and intestinal motility in mice, and the decrease of 5-HT, thereby changing the intestinal movement resulting in mucosal protective barrier damage,thereby causing changes in intestinal movement and the destruction of the intestinal mucosal protective barrier, which eventually resulted in constipation. The occurrence of constipation could be improved by regulating balance of intestinal flora, increasing the diversity of flora, and reducing the genus of opportunistic pathogens.
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spelling pubmed-88068462022-02-02 The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation Fu, Ruibiao Li, Zhongpeng Zhou, Rui Li, Chaoyang Shao, Shuai Li, Jin Bioengineered Research Paper To explore mechanism of intestinal flora dysregulation promoting constipation, 60 specific pathogen-free (SPF) mice were used as research objects and were treated with constipation population fecal fluid gavage and distilled water gavage. Then, relationship between intestinal dysregulation and constipation in mice with biofilm-mediated intestinal flora was investigated in vitro. The results showed that recombinant serotonin transporter (SERT) messenger ribonucleic acid (mRNA) level of the constipation population fecal fluid gavage group and the relative expression level of SERT mRNA were 1.61 ± 0.08 and 1.49 ± 0.06, which were higher markedly than those of distilled water group (P < 0.05). The level of 5-hydroxytryptamine (5-HT) in colonic tissue of the constipation population fecal fluid gavage group was 145.36 ± 14.12 ng/mL, and the expression level of 5-HT on the surface of epithelial cells of biofilm-positive colonic tissue was 20.11 ± 2.03, which were significantly lower than those of the distilled water group, with statistical significance (P < 0.05). Besides, the microbial sequencing of fecal flora indicated that The Akk and bacteroidetes ofconstipation population fecal fluid gavage group were higher hugely than those of distilled water group (P < 0.05).In conclusion, after the occurrence of constipation, the diversity of intestinal microflora decreased, and the probiotics reduced. Iintestinal microflora dysregulation would lead to increase of SERT expression level in defecation function and intestinal motility in mice, and the decrease of 5-HT, thereby changing the intestinal movement resulting in mucosal protective barrier damage,thereby causing changes in intestinal movement and the destruction of the intestinal mucosal protective barrier, which eventually resulted in constipation. The occurrence of constipation could be improved by regulating balance of intestinal flora, increasing the diversity of flora, and reducing the genus of opportunistic pathogens. Taylor & Francis 2021-09-13 /pmc/articles/PMC8806846/ /pubmed/34517785 http://dx.doi.org/10.1080/21655979.2021.1973356 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Fu, Ruibiao
Li, Zhongpeng
Zhou, Rui
Li, Chaoyang
Shao, Shuai
Li, Jin
The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
title The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
title_full The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
title_fullStr The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
title_full_unstemmed The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
title_short The mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
title_sort mechanism of intestinal flora dysregulation mediated by intestinal bacterial biofilm to induce constipation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806846/
https://www.ncbi.nlm.nih.gov/pubmed/34517785
http://dx.doi.org/10.1080/21655979.2021.1973356
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