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Interkit Reproducibility of the Indirect Immunofluorescence Assay on HEp-2 Cells Depends on the Immunofluorescence Reactivity Intensity and Pattern

INTRODUCTION: The indirect immunofluorescence assay on HEp-2 cells (HEp-2/IFA) is used worldwide for screening for autoantibodies to cellular antigens. Cell culture and fixation methods influence the cell distribution of autoantigens and the preservation of epitopes. Therefore, discrepancy of result...

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Detalles Bibliográficos
Autores principales:	Silva, Mônica Jesus, Dellavance, Alessandra, Baldo, Danielle Cristiane, Rodrigues, Silvia Helena, Grecco, Marcelle, Prado, Monica Simon, Agustinelli, Renan, Andrade, Luís Eduardo Coelho
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Immunology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8807640/ https://www.ncbi.nlm.nih.gov/pubmed/35126363 http://dx.doi.org/10.3389/fimmu.2021.798322

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