Better method for detection of CD30: Immunohistochemistry or flow cytometry?

We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was mo...

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Autores principales: Higashi, Morihiro, Kikuchi, Jun, Murakami, Chiaki, Takayanagi, Natsuko, Momose, Shuji, Kizaki, Masahiro, Tamaru, Jun-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: JSLRT 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808110/
https://www.ncbi.nlm.nih.gov/pubmed/34511584
http://dx.doi.org/10.3960/jslrt.21019
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author Higashi, Morihiro
Kikuchi, Jun
Murakami, Chiaki
Takayanagi, Natsuko
Momose, Shuji
Kizaki, Masahiro
Tamaru, Jun-ichi
author_facet Higashi, Morihiro
Kikuchi, Jun
Murakami, Chiaki
Takayanagi, Natsuko
Momose, Shuji
Kizaki, Masahiro
Tamaru, Jun-ichi
author_sort Higashi, Morihiro
collection PubMed
description We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expression. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.
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spelling pubmed-88081102022-02-15 Better method for detection of CD30: Immunohistochemistry or flow cytometry? Higashi, Morihiro Kikuchi, Jun Murakami, Chiaki Takayanagi, Natsuko Momose, Shuji Kizaki, Masahiro Tamaru, Jun-ichi J Clin Exp Hematop Short Communication We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expression. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30. JSLRT 2021-09-10 /pmc/articles/PMC8808110/ /pubmed/34511584 http://dx.doi.org/10.3960/jslrt.21019 Text en © 2021 by The Japanese Society for Lymphoreticular Tissue Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution ShareAlike (CC BY-NC-SA) 4.0 License.
spellingShingle Short Communication
Higashi, Morihiro
Kikuchi, Jun
Murakami, Chiaki
Takayanagi, Natsuko
Momose, Shuji
Kizaki, Masahiro
Tamaru, Jun-ichi
Better method for detection of CD30: Immunohistochemistry or flow cytometry?
title Better method for detection of CD30: Immunohistochemistry or flow cytometry?
title_full Better method for detection of CD30: Immunohistochemistry or flow cytometry?
title_fullStr Better method for detection of CD30: Immunohistochemistry or flow cytometry?
title_full_unstemmed Better method for detection of CD30: Immunohistochemistry or flow cytometry?
title_short Better method for detection of CD30: Immunohistochemistry or flow cytometry?
title_sort better method for detection of cd30: immunohistochemistry or flow cytometry?
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808110/
https://www.ncbi.nlm.nih.gov/pubmed/34511584
http://dx.doi.org/10.3960/jslrt.21019
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