The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos

BACKGROUND: Somatic cell nuclear transfer (SCNT) is an approach for the propagation of elite animals. In vitro condi- tion, especially the composition of culture media has a profound effect on the developmental competency of in vitro derived e mbryos. There are limited studies evaluating the effect...

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Autores principales: Hajian, Mehdi, Jafarpour, Farnoosh, Aghamiri, Sayed Morteza, Varnosfaderani, Shiva Rouhollahi, Andani, Mohsen Rahimi, Nasr-Esfahani, Mohammad Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808249/
https://www.ncbi.nlm.nih.gov/pubmed/35103428
http://dx.doi.org/10.22074/IJFS.2021.531302.1130
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author Hajian, Mehdi
Jafarpour, Farnoosh
Aghamiri, Sayed Morteza
Varnosfaderani, Shiva Rouhollahi
Andani, Mohsen Rahimi
Nasr-Esfahani, Mohammad Hossein
author_facet Hajian, Mehdi
Jafarpour, Farnoosh
Aghamiri, Sayed Morteza
Varnosfaderani, Shiva Rouhollahi
Andani, Mohsen Rahimi
Nasr-Esfahani, Mohammad Hossein
author_sort Hajian, Mehdi
collection PubMed
description BACKGROUND: Somatic cell nuclear transfer (SCNT) is an approach for the propagation of elite animals. In vitro condi- tion, especially the composition of culture media has a profound effect on the developmental competency of in vitro derived e mbryos. There are limited studies evaluating the effect of culture media on SCNT outcomes. To address this gap, we compare the effect of two culture media synthetic oviduct fluid (SOF) vs. commercial bracket-oliphant (BO) on developmental comptenecy. MATERIALS AND METHODS: In this experimental study, embryos derived from in vitro fertilized (IVF) and SCNT were cultured in both BO and SOF media for 7 days. In addition to the assessment of cleavage and blastocyst on day 3, and 7, the quantitative expression of 16 genes in theresultant blastocysts were assessed. The resultant SCNT blastocysts from SOF and BO groups were also transferred to the synchronized recipient for developmental competency to term. RESULTS: The blastocyst rate in the BO medium was significantly higher than that of the SOF medium in the SCNT group (P<0.05). All of the examined genes showed increased expression levels in SCNT blastocyst in both media compared to IVF Blastocyst. In the IVF group, Oct4, Bmpr1, and Gcn5 showed significantly higher expression in the SOF medium com- pared to the BO medium while Akt, Fgfr4, Sox2 showed significantly lower expression in the SOF medium compared to the BO medium. In the SCNT group, Fgfr4, Gcn5, Fzd, Ctnnb, Bmpr1, and Fgfr4 showed significantly higher expression in SOF compared to BO derived blastocyst. CONCLUSION: It appears that in SCNT blastocysts, gene regulation is less controlled compared to IVF ones, irrespective of the type of medium. In addition, there are differences regarding certain genes expressions between IVF and SCNT derived blas- tocysts between SOF and BO, reiterating that culture composition affects developmental competency and gene expression.
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spelling pubmed-88082492022-05-02 The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos Hajian, Mehdi Jafarpour, Farnoosh Aghamiri, Sayed Morteza Varnosfaderani, Shiva Rouhollahi Andani, Mohsen Rahimi Nasr-Esfahani, Mohammad Hossein Int J Fertil Steril Original Article BACKGROUND: Somatic cell nuclear transfer (SCNT) is an approach for the propagation of elite animals. In vitro condi- tion, especially the composition of culture media has a profound effect on the developmental competency of in vitro derived e mbryos. There are limited studies evaluating the effect of culture media on SCNT outcomes. To address this gap, we compare the effect of two culture media synthetic oviduct fluid (SOF) vs. commercial bracket-oliphant (BO) on developmental comptenecy. MATERIALS AND METHODS: In this experimental study, embryos derived from in vitro fertilized (IVF) and SCNT were cultured in both BO and SOF media for 7 days. In addition to the assessment of cleavage and blastocyst on day 3, and 7, the quantitative expression of 16 genes in theresultant blastocysts were assessed. The resultant SCNT blastocysts from SOF and BO groups were also transferred to the synchronized recipient for developmental competency to term. RESULTS: The blastocyst rate in the BO medium was significantly higher than that of the SOF medium in the SCNT group (P<0.05). All of the examined genes showed increased expression levels in SCNT blastocyst in both media compared to IVF Blastocyst. In the IVF group, Oct4, Bmpr1, and Gcn5 showed significantly higher expression in the SOF medium com- pared to the BO medium while Akt, Fgfr4, Sox2 showed significantly lower expression in the SOF medium compared to the BO medium. In the SCNT group, Fgfr4, Gcn5, Fzd, Ctnnb, Bmpr1, and Fgfr4 showed significantly higher expression in SOF compared to BO derived blastocyst. CONCLUSION: It appears that in SCNT blastocysts, gene regulation is less controlled compared to IVF ones, irrespective of the type of medium. In addition, there are differences regarding certain genes expressions between IVF and SCNT derived blas- tocysts between SOF and BO, reiterating that culture composition affects developmental competency and gene expression. Royan Institute 2022 2022-01-17 /pmc/articles/PMC8808249/ /pubmed/35103428 http://dx.doi.org/10.22074/IJFS.2021.531302.1130 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hajian, Mehdi
Jafarpour, Farnoosh
Aghamiri, Sayed Morteza
Varnosfaderani, Shiva Rouhollahi
Andani, Mohsen Rahimi
Nasr-Esfahani, Mohammad Hossein
The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos
title The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos
title_full The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos
title_fullStr The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos
title_full_unstemmed The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos
title_short The Impact of Two Embryo Culture Media, Synthetic Oviduct Fluid and Commercial BO, on pre-and post-Implantation Development of Cloned SAANEN Goat Embryos
title_sort impact of two embryo culture media, synthetic oviduct fluid and commercial bo, on pre-and post-implantation development of cloned saanen goat embryos
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808249/
https://www.ncbi.nlm.nih.gov/pubmed/35103428
http://dx.doi.org/10.22074/IJFS.2021.531302.1130
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