Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics

The assembly of mitochondrial respiratory complexes into supercomplexes has significant implications for mitochondrial function. This protocol details mitochondrial isolation from mouse tissues and the use of blue native gel electrophoresis (BN-PAGE) to separate pre-identified mitochondrial supercom...

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Detalles Bibliográficos
Autores principales: Moreno-Justicia, Roger, Gonzalez-Franquesa, Alba, Stocks, Ben, Deshmukh, Atul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8808288/
https://www.ncbi.nlm.nih.gov/pubmed/35128478
http://dx.doi.org/10.1016/j.xpro.2022.101135

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