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Generation of bright monomeric red fluorescent proteins via computational design of enhanced chromophore packing

Red fluorescent proteins (RFPs) have found widespread application in chemical and biological research due to their longer emission wavelengths. Here, we use computational protein design to increase the quantum yield and thereby brightness of a dim monomeric RFP (mRojoA, quantum yield = 0.02) by opti...

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Detalles Bibliográficos
Autores principales:	Legault, Sandrine, Fraser-Halberg, Derek P., McAnelly, Ralph L., Eason, Matthew G., Thompson, Michael C., Chica, Roberto A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2022
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809391/ https://www.ncbi.nlm.nih.gov/pubmed/35222925 http://dx.doi.org/10.1039/d1sc05088e

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