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The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1

Ovarian cancer (OC) is one of the most common malignancies of the female reproductive system. The miRNA miR-582-3p is associated with a variety of tumors, and the aim of this study was to investigate the role and mechanisms of miR-582-3p specifically in ovarian carcinogenesis and progression. Low ex...

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Autores principales: Dai, Tianyu, Liang, Junhui, Liu, Wei, Zou, Yonghui, Niu, Feifei, Li, Mengqing, Zhang, Haomeng, Li, Changzhong, Fan, Mingjun, Cui, Guoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810093/
https://www.ncbi.nlm.nih.gov/pubmed/34793263
http://dx.doi.org/10.1080/21655979.2021.2003662
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author Dai, Tianyu
Liang, Junhui
Liu, Wei
Zou, Yonghui
Niu, Feifei
Li, Mengqing
Zhang, Haomeng
Li, Changzhong
Fan, Mingjun
Cui, Guoying
author_facet Dai, Tianyu
Liang, Junhui
Liu, Wei
Zou, Yonghui
Niu, Feifei
Li, Mengqing
Zhang, Haomeng
Li, Changzhong
Fan, Mingjun
Cui, Guoying
author_sort Dai, Tianyu
collection PubMed
description Ovarian cancer (OC) is one of the most common malignancies of the female reproductive system. The miRNA miR-582-3p is associated with a variety of tumors, and the aim of this study was to investigate the role and mechanisms of miR-582-3p specifically in ovarian carcinogenesis and progression. Low expression of miR-582-3p was noted in OC tissue and cell lines, and lower expression of miR-582-3p correlated with lower overall survival in OC patients. Knockdown of miR-582-3p promoted the proliferation and migration of OC cells, while overexpression inhibited them. TUG1, a long non-coding RNA, was found to bind to miR-582-3p, and inhibition of lncRNA TUG1 decreased viability and migration and weakened the effect of miR-582-3p knockdown in OC cells. Implantation of OC cells with reduced miR-582-3p caused increased tumor growth, while lncRNA TUG1 knockdown suppressed tumor growth and relieved the impact of reduced miR-582-3p in vivo. Phosphorylation of AKT and mTOR were significantly enhanced with decreased miR-582-3p expression, but lncRNA TUG1 knockdown attenuated this trend in vitro and in vivo. The novel miR-582-3p represses the malignant properties of OC via the AKT/mTOR signaling pathway by targeting lncRNA TUG1. This axis may represent valuable prognostic biomarkers and therapeutic targets for OC.
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spelling pubmed-88100932022-02-03 The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1 Dai, Tianyu Liang, Junhui Liu, Wei Zou, Yonghui Niu, Feifei Li, Mengqing Zhang, Haomeng Li, Changzhong Fan, Mingjun Cui, Guoying Bioengineered Research Paper Ovarian cancer (OC) is one of the most common malignancies of the female reproductive system. The miRNA miR-582-3p is associated with a variety of tumors, and the aim of this study was to investigate the role and mechanisms of miR-582-3p specifically in ovarian carcinogenesis and progression. Low expression of miR-582-3p was noted in OC tissue and cell lines, and lower expression of miR-582-3p correlated with lower overall survival in OC patients. Knockdown of miR-582-3p promoted the proliferation and migration of OC cells, while overexpression inhibited them. TUG1, a long non-coding RNA, was found to bind to miR-582-3p, and inhibition of lncRNA TUG1 decreased viability and migration and weakened the effect of miR-582-3p knockdown in OC cells. Implantation of OC cells with reduced miR-582-3p caused increased tumor growth, while lncRNA TUG1 knockdown suppressed tumor growth and relieved the impact of reduced miR-582-3p in vivo. Phosphorylation of AKT and mTOR were significantly enhanced with decreased miR-582-3p expression, but lncRNA TUG1 knockdown attenuated this trend in vitro and in vivo. The novel miR-582-3p represses the malignant properties of OC via the AKT/mTOR signaling pathway by targeting lncRNA TUG1. This axis may represent valuable prognostic biomarkers and therapeutic targets for OC. Taylor & Francis 2021-12-02 /pmc/articles/PMC8810093/ /pubmed/34793263 http://dx.doi.org/10.1080/21655979.2021.2003662 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Dai, Tianyu
Liang, Junhui
Liu, Wei
Zou, Yonghui
Niu, Feifei
Li, Mengqing
Zhang, Haomeng
Li, Changzhong
Fan, Mingjun
Cui, Guoying
The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1
title The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1
title_full The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1
title_fullStr The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1
title_full_unstemmed The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1
title_short The miRNA mir-582-3p suppresses ovarian cancer progression by targeting AKT/MTOR signaling via lncRNA TUG1
title_sort mirna mir-582-3p suppresses ovarian cancer progression by targeting akt/mtor signaling via lncrna tug1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810093/
https://www.ncbi.nlm.nih.gov/pubmed/34793263
http://dx.doi.org/10.1080/21655979.2021.2003662
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