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Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast
Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer or...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810105/ https://www.ncbi.nlm.nih.gov/pubmed/34874791 http://dx.doi.org/10.1080/21655979.2021.1974809 |
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author | Luo, XiangRong She, JianTao Xu, Tao Zhou, Yuan Xu, ChuanBo Jiang, JianPing Li, TianGang Liu, Huajiang Shen, Hui Yin, Bolong Dai, Bin |
author_facet | Luo, XiangRong She, JianTao Xu, Tao Zhou, Yuan Xu, ChuanBo Jiang, JianPing Li, TianGang Liu, Huajiang Shen, Hui Yin, Bolong Dai, Bin |
author_sort | Luo, XiangRong |
collection | PubMed |
description | Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer organoids from the surgical tumor samples of an elderly Chinese woman with an AME of the breast. Our findings confirmed the successful establishment of organoids from an AME of the breast of this patient. A short tandem repeat analysis revealed that the DNA signature of the AME of the breast organoids matched the DNA signature of the original tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast AME showed sensitivity to paclitaxel and doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional breast cancer organoid culture platform from an AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of AME of the breast to identify possible treatments and increase awareness about this disease entity. |
format | Online Article Text |
id | pubmed-8810105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-88101052022-02-03 Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast Luo, XiangRong She, JianTao Xu, Tao Zhou, Yuan Xu, ChuanBo Jiang, JianPing Li, TianGang Liu, Huajiang Shen, Hui Yin, Bolong Dai, Bin Bioengineered Research Paper Adenomyoepithelioma (AME) of the breast is a rare tumor that is composed of proliferating epithelial and myoepithelial cells. The pathogenesis of AME remains unclear, and no breast cancer cells have been identified in such tumor tissues. In this study, we established patient-derived breast cancer organoids from the surgical tumor samples of an elderly Chinese woman with an AME of the breast. Our findings confirmed the successful establishment of organoids from an AME of the breast of this patient. A short tandem repeat analysis revealed that the DNA signature of the AME of the breast organoids matched the DNA signature of the original tumor specimen. Moreover, diameter assay confirmed that the organoids from the breast AME showed sensitivity to paclitaxel and doxorubicin treatments, which was similar to, but lesser than that of primary culture cells. In conclusion, we established an efficient 3-dimensional breast cancer organoid culture platform from an AME of the breast. This platform can be effectively used for exploring clinicopathological and genomic characteristics of AME of the breast to identify possible treatments and increase awareness about this disease entity. Taylor & Francis 2021-12-07 /pmc/articles/PMC8810105/ /pubmed/34874791 http://dx.doi.org/10.1080/21655979.2021.1974809 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Luo, XiangRong She, JianTao Xu, Tao Zhou, Yuan Xu, ChuanBo Jiang, JianPing Li, TianGang Liu, Huajiang Shen, Hui Yin, Bolong Dai, Bin Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
title | Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
title_full | Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
title_fullStr | Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
title_full_unstemmed | Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
title_short | Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
title_sort | establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810105/ https://www.ncbi.nlm.nih.gov/pubmed/34874791 http://dx.doi.org/10.1080/21655979.2021.1974809 |
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