Cargando…
Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma
In this study we report a novel specificity protein 1 (SP1)/microRNA-92b (miR-92b) feedback loop regulating the migration and invasion of head and neck squamous cell carcinoma (HNSCC). Microarray and real-time Polymerase Chain Reaction (PCR) were used to detect gene expression in HNSCC tissues and c...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810166/ https://www.ncbi.nlm.nih.gov/pubmed/34905435 http://dx.doi.org/10.1080/21655979.2021.2008698 |
_version_ | 1784644192999309312 |
---|---|
author | Pang, Pai Fang, Hui Wu, Hong Wang, Song Liu, Minda Jin, Shan Qi, Zhongzheng Li, Zhenning Liu, Fayu Sun, Changfu |
author_facet | Pang, Pai Fang, Hui Wu, Hong Wang, Song Liu, Minda Jin, Shan Qi, Zhongzheng Li, Zhenning Liu, Fayu Sun, Changfu |
author_sort | Pang, Pai |
collection | PubMed |
description | In this study we report a novel specificity protein 1 (SP1)/microRNA-92b (miR-92b) feedback loop regulating the migration and invasion of head and neck squamous cell carcinoma (HNSCC). Microarray and real-time Polymerase Chain Reaction (PCR) were used to detect gene expression in HNSCC tissues and cell lines. Transwell migration, invasion, wound healing and cell counting kit – 8 (CCK-8) cell assays were used to compare cell migration, invasion and proliferation abilities. Chromatin Immunoprecipitation (ChIP) assays were used to detect SP1 binding to the miR-92b promoter. Western blot was used to detect protein levels. An in vivo tumorigenesis experiment was used to evaluate the effect of SP1 knockdown on tumor growth and protein levels were evaluated by immunohistochemistry. We found that the miR-92b expression level was elevated in HNSCC primary focus tissue compared with adjacent normal tissue, and a higher level of miR-92b was related to a higher clinical stage and worse prognosis of HNSCC patients. MiR-92b and SP1 mutually promoted each expression and cooperatively facilitated the migration, invasion and proliferation of HNSCC cells. A decreased level of SP1/miR-92b resulted in a restraint of in vivo tumor growth. In conclusion, our results suggest that the SP1/miR-92b feedback loop generally promotes HNSCC invasion and metastasis, thus presenting a possible therapeutic target in the treatment of HNSCC patients. |
format | Online Article Text |
id | pubmed-8810166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-88101662022-02-03 Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma Pang, Pai Fang, Hui Wu, Hong Wang, Song Liu, Minda Jin, Shan Qi, Zhongzheng Li, Zhenning Liu, Fayu Sun, Changfu Bioengineered Research Paper In this study we report a novel specificity protein 1 (SP1)/microRNA-92b (miR-92b) feedback loop regulating the migration and invasion of head and neck squamous cell carcinoma (HNSCC). Microarray and real-time Polymerase Chain Reaction (PCR) were used to detect gene expression in HNSCC tissues and cell lines. Transwell migration, invasion, wound healing and cell counting kit – 8 (CCK-8) cell assays were used to compare cell migration, invasion and proliferation abilities. Chromatin Immunoprecipitation (ChIP) assays were used to detect SP1 binding to the miR-92b promoter. Western blot was used to detect protein levels. An in vivo tumorigenesis experiment was used to evaluate the effect of SP1 knockdown on tumor growth and protein levels were evaluated by immunohistochemistry. We found that the miR-92b expression level was elevated in HNSCC primary focus tissue compared with adjacent normal tissue, and a higher level of miR-92b was related to a higher clinical stage and worse prognosis of HNSCC patients. MiR-92b and SP1 mutually promoted each expression and cooperatively facilitated the migration, invasion and proliferation of HNSCC cells. A decreased level of SP1/miR-92b resulted in a restraint of in vivo tumor growth. In conclusion, our results suggest that the SP1/miR-92b feedback loop generally promotes HNSCC invasion and metastasis, thus presenting a possible therapeutic target in the treatment of HNSCC patients. Taylor & Francis 2021-12-14 /pmc/articles/PMC8810166/ /pubmed/34905435 http://dx.doi.org/10.1080/21655979.2021.2008698 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Pang, Pai Fang, Hui Wu, Hong Wang, Song Liu, Minda Jin, Shan Qi, Zhongzheng Li, Zhenning Liu, Fayu Sun, Changfu Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
title | Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
title_full | Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
title_fullStr | Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
title_full_unstemmed | Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
title_short | Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
title_sort | specificity protein 1/microrna-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810166/ https://www.ncbi.nlm.nih.gov/pubmed/34905435 http://dx.doi.org/10.1080/21655979.2021.2008698 |
work_keys_str_mv | AT pangpai specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT fanghui specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT wuhong specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT wangsong specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT liuminda specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT jinshan specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT qizhongzheng specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT lizhenning specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT liufayu specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma AT sunchangfu specificityprotein1microrna92bformsafeedbacklooppromotingthemigrationandinvasionofheadandnecksquamouscellcarcinoma |