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Expression and role of microRNA-212/nuclear factor I-A in depressive mice

Depression is characterized by persistent depressed mood and cognitive dysfunction, severely impacting human health. In the present study, we aimed to explore the role and mechanism of microRNA (miR)-212 in depression in vivo. Chronic unpredictable mild stress (CUMS) mice were established, and depre...

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Autores principales: Si, Liang, Wang, Yanyan, Liu, Min, Yang, Lifeng, Zhang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810195/
https://www.ncbi.nlm.nih.gov/pubmed/34889698
http://dx.doi.org/10.1080/21655979.2021.2009964
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author Si, Liang
Wang, Yanyan
Liu, Min
Yang, Lifeng
Zhang, Li
author_facet Si, Liang
Wang, Yanyan
Liu, Min
Yang, Lifeng
Zhang, Li
author_sort Si, Liang
collection PubMed
description Depression is characterized by persistent depressed mood and cognitive dysfunction, severely impacting human health. In the present study, we aimed to explore the role and mechanism of microRNA (miR)-212 in depression in vivo. Chronic unpredictable mild stress (CUMS) mice were established, and depression-like behaviors were confirmed using the forced swimming test (FST), sucrose preference test (SPT), and the tail suspension test (TST). Next, the expression of miR-212 and its potential target, i.e., nuclear factor I-A (NFIA), was verified using quantitative reverse transcription (qRT)-PCR analysis and Western blotting in CUMS mice. The effects of miR-212 and NFIA on depression-like behaviors, inflammatory response, and neuronal apoptosis were examined using FST, TST, SPT, enzyme-linked immunosorbent assay (ELISA) assay, and flow cytometry analysis. Finally, the relationship between miR-212 and NFIA was examined using a dual-luciferase reporter assay. Based on our findings, miR-212 was significantly upregulated, while NFIA was downregulated in CUMS mice. miR-212 overexpression could suppress the CUMS-induced weight loss, immobility time in FST and TST, and increased hippocampal neuronal apoptosis and pro-inflammatory cytokines levels. In addition, NFIA upregulation could partially reverse the effects of miR-212 mimic in CUMS mice. Accordingly, miR-212 could ameliorate CUMS-induced depression-like behavior in mice by targeting NFIA, indicating its protective role in depression.
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spelling pubmed-88101952022-02-03 Expression and role of microRNA-212/nuclear factor I-A in depressive mice Si, Liang Wang, Yanyan Liu, Min Yang, Lifeng Zhang, Li Bioengineered Research Paper Depression is characterized by persistent depressed mood and cognitive dysfunction, severely impacting human health. In the present study, we aimed to explore the role and mechanism of microRNA (miR)-212 in depression in vivo. Chronic unpredictable mild stress (CUMS) mice were established, and depression-like behaviors were confirmed using the forced swimming test (FST), sucrose preference test (SPT), and the tail suspension test (TST). Next, the expression of miR-212 and its potential target, i.e., nuclear factor I-A (NFIA), was verified using quantitative reverse transcription (qRT)-PCR analysis and Western blotting in CUMS mice. The effects of miR-212 and NFIA on depression-like behaviors, inflammatory response, and neuronal apoptosis were examined using FST, TST, SPT, enzyme-linked immunosorbent assay (ELISA) assay, and flow cytometry analysis. Finally, the relationship between miR-212 and NFIA was examined using a dual-luciferase reporter assay. Based on our findings, miR-212 was significantly upregulated, while NFIA was downregulated in CUMS mice. miR-212 overexpression could suppress the CUMS-induced weight loss, immobility time in FST and TST, and increased hippocampal neuronal apoptosis and pro-inflammatory cytokines levels. In addition, NFIA upregulation could partially reverse the effects of miR-212 mimic in CUMS mice. Accordingly, miR-212 could ameliorate CUMS-induced depression-like behavior in mice by targeting NFIA, indicating its protective role in depression. Taylor & Francis 2021-12-10 /pmc/articles/PMC8810195/ /pubmed/34889698 http://dx.doi.org/10.1080/21655979.2021.2009964 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Si, Liang
Wang, Yanyan
Liu, Min
Yang, Lifeng
Zhang, Li
Expression and role of microRNA-212/nuclear factor I-A in depressive mice
title Expression and role of microRNA-212/nuclear factor I-A in depressive mice
title_full Expression and role of microRNA-212/nuclear factor I-A in depressive mice
title_fullStr Expression and role of microRNA-212/nuclear factor I-A in depressive mice
title_full_unstemmed Expression and role of microRNA-212/nuclear factor I-A in depressive mice
title_short Expression and role of microRNA-212/nuclear factor I-A in depressive mice
title_sort expression and role of microrna-212/nuclear factor i-a in depressive mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810195/
https://www.ncbi.nlm.nih.gov/pubmed/34889698
http://dx.doi.org/10.1080/21655979.2021.2009964
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