Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines

C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriat...

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Autores principales: KANEI, Toshitaka, IWATA, Munetaka, KAMISHINA, Hiroaki, MIZUNO, Takuya, MAEDA, Sadatoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810311/
https://www.ncbi.nlm.nih.gov/pubmed/34866095
http://dx.doi.org/10.1292/jvms.21-0468
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author KANEI, Toshitaka
IWATA, Munetaka
KAMISHINA, Hiroaki
MIZUNO, Takuya
MAEDA, Sadatoshi
author_facet KANEI, Toshitaka
IWATA, Munetaka
KAMISHINA, Hiroaki
MIZUNO, Takuya
MAEDA, Sadatoshi
author_sort KANEI, Toshitaka
collection PubMed
description C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriate antibodies. In the present study, a fusion protein of C-C chemokine ligand 19 (CCL19) was employed as an alternative method to CCR7 antibodies. The fusion CCL19 protein specifically detected CCR7 expressed in canine lymphoma cell lines, which showed active chemotaxis to both canine and mouse ligands. The present study will help further research on the involvement of canine CCR7 in LN metastasis.
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spelling pubmed-88103112022-02-25 Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines KANEI, Toshitaka IWATA, Munetaka KAMISHINA, Hiroaki MIZUNO, Takuya MAEDA, Sadatoshi J Vet Med Sci Internal Medicine C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriate antibodies. In the present study, a fusion protein of C-C chemokine ligand 19 (CCL19) was employed as an alternative method to CCR7 antibodies. The fusion CCL19 protein specifically detected CCR7 expressed in canine lymphoma cell lines, which showed active chemotaxis to both canine and mouse ligands. The present study will help further research on the involvement of canine CCR7 in LN metastasis. The Japanese Society of Veterinary Science 2021-12-03 2022-01 /pmc/articles/PMC8810311/ /pubmed/34866095 http://dx.doi.org/10.1292/jvms.21-0468 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
KANEI, Toshitaka
IWATA, Munetaka
KAMISHINA, Hiroaki
MIZUNO, Takuya
MAEDA, Sadatoshi
Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
title Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
title_full Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
title_fullStr Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
title_full_unstemmed Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
title_short Expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
title_sort expression and functional analysis of chemokine receptor 7 in canine lymphoma cell lines
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810311/
https://www.ncbi.nlm.nih.gov/pubmed/34866095
http://dx.doi.org/10.1292/jvms.21-0468
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