Cargando…
Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reac...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810321/ https://www.ncbi.nlm.nih.gov/pubmed/34853198 http://dx.doi.org/10.1292/jvms.21-0539 |
_version_ | 1784644229461442560 |
---|---|
author | KINOSHITA, Yuta KAKOI, Hironaga ISHIGE, Taichiro YAMANAKA, Takashi NIWA, Hidekazu UCHIDA-FUJII, Eri NUKADA, Toshio UENO, Takanori |
author_facet | KINOSHITA, Yuta KAKOI, Hironaga ISHIGE, Taichiro YAMANAKA, Takashi NIWA, Hidekazu UCHIDA-FUJII, Eri NUKADA, Toshio UENO, Takanori |
author_sort | KINOSHITA, Yuta |
collection | PubMed |
description | Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples. |
format | Online Article Text |
id | pubmed-8810321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-88103212022-02-25 Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis KINOSHITA, Yuta KAKOI, Hironaga ISHIGE, Taichiro YAMANAKA, Takashi NIWA, Hidekazu UCHIDA-FUJII, Eri NUKADA, Toshio UENO, Takanori J Vet Med Sci Bacteriology Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples. The Japanese Society of Veterinary Science 2021-12-02 2022-01 /pmc/articles/PMC8810321/ /pubmed/34853198 http://dx.doi.org/10.1292/jvms.21-0539 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Bacteriology KINOSHITA, Yuta KAKOI, Hironaga ISHIGE, Taichiro YAMANAKA, Takashi NIWA, Hidekazu UCHIDA-FUJII, Eri NUKADA, Toshio UENO, Takanori Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
title | Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
title_full | Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
title_fullStr | Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
title_full_unstemmed | Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
title_short | Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis |
title_sort | comparison of seven nucleic acid amplification tests for detection of taylorella equigenitalis |
topic | Bacteriology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810321/ https://www.ncbi.nlm.nih.gov/pubmed/34853198 http://dx.doi.org/10.1292/jvms.21-0539 |
work_keys_str_mv | AT kinoshitayuta comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT kakoihironaga comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT ishigetaichiro comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT yamanakatakashi comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT niwahidekazu comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT uchidafujiieri comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT nukadatoshio comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis AT uenotakanori comparisonofsevennucleicacidamplificationtestsfordetectionoftaylorellaequigenitalis |