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Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis

Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reac...

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Autores principales: KINOSHITA, Yuta, KAKOI, Hironaga, ISHIGE, Taichiro, YAMANAKA, Takashi, NIWA, Hidekazu, UCHIDA-FUJII, Eri, NUKADA, Toshio, UENO, Takanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810321/
https://www.ncbi.nlm.nih.gov/pubmed/34853198
http://dx.doi.org/10.1292/jvms.21-0539
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author KINOSHITA, Yuta
KAKOI, Hironaga
ISHIGE, Taichiro
YAMANAKA, Takashi
NIWA, Hidekazu
UCHIDA-FUJII, Eri
NUKADA, Toshio
UENO, Takanori
author_facet KINOSHITA, Yuta
KAKOI, Hironaga
ISHIGE, Taichiro
YAMANAKA, Takashi
NIWA, Hidekazu
UCHIDA-FUJII, Eri
NUKADA, Toshio
UENO, Takanori
author_sort KINOSHITA, Yuta
collection PubMed
description Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.
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spelling pubmed-88103212022-02-25 Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis KINOSHITA, Yuta KAKOI, Hironaga ISHIGE, Taichiro YAMANAKA, Takashi NIWA, Hidekazu UCHIDA-FUJII, Eri NUKADA, Toshio UENO, Takanori J Vet Med Sci Bacteriology Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples. The Japanese Society of Veterinary Science 2021-12-02 2022-01 /pmc/articles/PMC8810321/ /pubmed/34853198 http://dx.doi.org/10.1292/jvms.21-0539 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
KINOSHITA, Yuta
KAKOI, Hironaga
ISHIGE, Taichiro
YAMANAKA, Takashi
NIWA, Hidekazu
UCHIDA-FUJII, Eri
NUKADA, Toshio
UENO, Takanori
Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
title Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
title_full Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
title_fullStr Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
title_full_unstemmed Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
title_short Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis
title_sort comparison of seven nucleic acid amplification tests for detection of taylorella equigenitalis
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810321/
https://www.ncbi.nlm.nih.gov/pubmed/34853198
http://dx.doi.org/10.1292/jvms.21-0539
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