Cargando…
Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium
Modification of the titanium (Ti) surface is widely known to influence biological reactions such as protein adsorption and bacterial adhesion in vivo, ultimately controlling osseointegration. In this study, we sought to investigate the correlation of protein adsorption and bacterial adhesion with th...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810456/ https://www.ncbi.nlm.nih.gov/pubmed/35107647 http://dx.doi.org/10.1007/s10856-022-06646-7 |
_version_ | 1784644257002291200 |
---|---|
author | Zhong, Juan Li, Xuelian Yao, Yitong Zhou, Jing Cao, Shanshan Zhang, Xinping Jian, Yutao Zhao, Ke |
author_facet | Zhong, Juan Li, Xuelian Yao, Yitong Zhou, Jing Cao, Shanshan Zhang, Xinping Jian, Yutao Zhao, Ke |
author_sort | Zhong, Juan |
collection | PubMed |
description | Modification of the titanium (Ti) surface is widely known to influence biological reactions such as protein adsorption and bacterial adhesion in vivo, ultimately controlling osseointegration. In this study, we sought to investigate the correlation of protein adsorption and bacterial adhesion with the nanoporous structure of acid-alkali-treated Ti implants, shedding light on the modification of Ti implants to promote osseointegration. We fabricated nontreated porous Ti (NTPT) by powder metallurgy and immersed it in mixed acids and NaOH to obtain acid-alkali-treated porous Ti (AAPT). Nontreated dense sample (NTDT) served as control. Our results showed that nanopores were formed after acid-alkali treatment. AAPT showed a higher specific surface area and became much more hydrophilic than NTPT and NTDT (p < 0.001). Compared to dense samples, porous samples exhibited a lower zeta potential and higher adsorbed protein level at each time point within 120 min (p < 0.001). AAPT formed a thicker protein layer by serum precoating than NTPT and NTDT (p < 0.001). The main adsorbed proteins on AAPT and NTPT were albumin, α1 antitrypsin, transferrin, apolipoprotein A1, complement C3 and haptoglobin α1 chain. The amounts of bacteria adhering to the serum-precoated samples were lower than those adhering to the nonprecoated samples (p < 0.05). Lower-molecular-weight proteins showed higher affinity to porous Ti. In conclusion, acid-alkali treatment facilitated protein adsorption by porous Ti, and the protein coating tended to prevent bacteria from adhering. These findings may be utilized for Ti implant modification aimed at reducing bacterial adhesion and enhancing osseointegration. [Figure: see text] |
format | Online Article Text |
id | pubmed-8810456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-88104562022-02-22 Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium Zhong, Juan Li, Xuelian Yao, Yitong Zhou, Jing Cao, Shanshan Zhang, Xinping Jian, Yutao Zhao, Ke J Mater Sci Mater Med Biomaterials Synthesis and Characterization Modification of the titanium (Ti) surface is widely known to influence biological reactions such as protein adsorption and bacterial adhesion in vivo, ultimately controlling osseointegration. In this study, we sought to investigate the correlation of protein adsorption and bacterial adhesion with the nanoporous structure of acid-alkali-treated Ti implants, shedding light on the modification of Ti implants to promote osseointegration. We fabricated nontreated porous Ti (NTPT) by powder metallurgy and immersed it in mixed acids and NaOH to obtain acid-alkali-treated porous Ti (AAPT). Nontreated dense sample (NTDT) served as control. Our results showed that nanopores were formed after acid-alkali treatment. AAPT showed a higher specific surface area and became much more hydrophilic than NTPT and NTDT (p < 0.001). Compared to dense samples, porous samples exhibited a lower zeta potential and higher adsorbed protein level at each time point within 120 min (p < 0.001). AAPT formed a thicker protein layer by serum precoating than NTPT and NTDT (p < 0.001). The main adsorbed proteins on AAPT and NTPT were albumin, α1 antitrypsin, transferrin, apolipoprotein A1, complement C3 and haptoglobin α1 chain. The amounts of bacteria adhering to the serum-precoated samples were lower than those adhering to the nonprecoated samples (p < 0.05). Lower-molecular-weight proteins showed higher affinity to porous Ti. In conclusion, acid-alkali treatment facilitated protein adsorption by porous Ti, and the protein coating tended to prevent bacteria from adhering. These findings may be utilized for Ti implant modification aimed at reducing bacterial adhesion and enhancing osseointegration. [Figure: see text] Springer US 2022-02-02 2022 /pmc/articles/PMC8810456/ /pubmed/35107647 http://dx.doi.org/10.1007/s10856-022-06646-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biomaterials Synthesis and Characterization Zhong, Juan Li, Xuelian Yao, Yitong Zhou, Jing Cao, Shanshan Zhang, Xinping Jian, Yutao Zhao, Ke Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
title | Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
title_full | Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
title_fullStr | Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
title_full_unstemmed | Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
title_short | Effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
title_sort | effect of acid-alkali treatment on serum protein adsorption and bacterial adhesion to porous titanium |
topic | Biomaterials Synthesis and Characterization |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810456/ https://www.ncbi.nlm.nih.gov/pubmed/35107647 http://dx.doi.org/10.1007/s10856-022-06646-7 |
work_keys_str_mv | AT zhongjuan effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT lixuelian effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT yaoyitong effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT zhoujing effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT caoshanshan effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT zhangxinping effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT jianyutao effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium AT zhaoke effectofacidalkalitreatmentonserumproteinadsorptionandbacterialadhesiontoporoustitanium |