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Protocol for ex vivo time lapse imaging of Drosophila melanogaster cytonemes

This protocol describes how to image time and spatially resolved time lapses of Drosophila air sac primordium (ASP) cytonemes in ex vivo cultures of wing imaginal discs. It describes how to manually measure the length of cytonemes using custom-made FIJI/ImageJ tools, and to analyze data using R/R-St...

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Detalles Bibliográficos
Autores principales:	Barbosa, Guilherme O., Kornberg, Thomas B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810567/ https://www.ncbi.nlm.nih.gov/pubmed/35141564 http://dx.doi.org/10.1016/j.xpro.2022.101138

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