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In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)

Dragon’s head plant (Lallemantia iberica), is a flowering species belongs to the mint family (Lamiaceae). The species contains valuable essential oils, mucilage and oil which are used in pharmaceutical and food industries. Tissue culture is a feasible strategy to attain large‐scale production of pla...

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Autores principales: Ebrahimzadegan, Rahman, Maroufi, Asad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810885/
https://www.ncbi.nlm.nih.gov/pubmed/35110618
http://dx.doi.org/10.1038/s41598-022-05776-w
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author Ebrahimzadegan, Rahman
Maroufi, Asad
author_facet Ebrahimzadegan, Rahman
Maroufi, Asad
author_sort Ebrahimzadegan, Rahman
collection PubMed
description Dragon’s head plant (Lallemantia iberica), is a flowering species belongs to the mint family (Lamiaceae). The species contains valuable essential oils, mucilage and oil which are used in pharmaceutical and food industries. Tissue culture is a feasible strategy to attain large‐scale production of plantlets with a huge potential to produce plants with superior quality. The objective of this study was to develop a simple and efficient method for regeneration and transformation of L. iberica. To reach this goal, the regeneration ability of various explants including leaf, cotyledonary node, hypocotyl and cotyledon segments was investigated in MS medium supplemented with diverse concentrations of NAA (Naphthalene acetic acid) and BAP (6-Benzyl Amino Purine). According to the results, cotyledonary nodes showed the best regeneration response. The maximum rate of regeneration (and number of induced shoots was achieved in 1 mg l(−1) BAP in combination with 0.05 mg l(−1) NAA from the cotyledonary nodes. Additionally, through the optimized regeneration technique Agrobacterium-mediated transformation of L. iberica was successfully accomplished. Gene transfer was assessed on leaf samples from regenerated plantlets under a fluorescent microscope to detect the GFP signals. Moreover, transgene integration and its expression were confirmed by PCR and RT-PCR analysis, respectively. The establishment of these efficient regeneration and genetic transformation methods paved the way for further application such as plant improvement, functional analysis and gene editing.
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spelling pubmed-88108852022-02-03 In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica) Ebrahimzadegan, Rahman Maroufi, Asad Sci Rep Article Dragon’s head plant (Lallemantia iberica), is a flowering species belongs to the mint family (Lamiaceae). The species contains valuable essential oils, mucilage and oil which are used in pharmaceutical and food industries. Tissue culture is a feasible strategy to attain large‐scale production of plantlets with a huge potential to produce plants with superior quality. The objective of this study was to develop a simple and efficient method for regeneration and transformation of L. iberica. To reach this goal, the regeneration ability of various explants including leaf, cotyledonary node, hypocotyl and cotyledon segments was investigated in MS medium supplemented with diverse concentrations of NAA (Naphthalene acetic acid) and BAP (6-Benzyl Amino Purine). According to the results, cotyledonary nodes showed the best regeneration response. The maximum rate of regeneration (and number of induced shoots was achieved in 1 mg l(−1) BAP in combination with 0.05 mg l(−1) NAA from the cotyledonary nodes. Additionally, through the optimized regeneration technique Agrobacterium-mediated transformation of L. iberica was successfully accomplished. Gene transfer was assessed on leaf samples from regenerated plantlets under a fluorescent microscope to detect the GFP signals. Moreover, transgene integration and its expression were confirmed by PCR and RT-PCR analysis, respectively. The establishment of these efficient regeneration and genetic transformation methods paved the way for further application such as plant improvement, functional analysis and gene editing. Nature Publishing Group UK 2022-02-02 /pmc/articles/PMC8810885/ /pubmed/35110618 http://dx.doi.org/10.1038/s41598-022-05776-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ebrahimzadegan, Rahman
Maroufi, Asad
In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)
title In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)
title_full In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)
title_fullStr In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)
title_full_unstemmed In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)
title_short In vitro regeneration and Agrobacterium-mediated genetic transformation of Dragon’s Head plant (Lallemantia iberica)
title_sort in vitro regeneration and agrobacterium-mediated genetic transformation of dragon’s head plant (lallemantia iberica)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8810885/
https://www.ncbi.nlm.nih.gov/pubmed/35110618
http://dx.doi.org/10.1038/s41598-022-05776-w
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