Cargando…

Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora

Chronic obstructive pulmonary disease (COPD) is a common obstructive respiratory disease characterized by persistent respiratory symptoms and limited airflow due to airway obstruction. The present study investigates the distribution characteristics of respiratory tract flora in both frequent and inf...

Descripción completa

Detalles Bibliográficos
Autores principales: Ke, Li, Chen, Luo, Yaling, Yuan, Can, Gao, Jun, Lin, Chuan, Zhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811034/
https://www.ncbi.nlm.nih.gov/pubmed/35127772
http://dx.doi.org/10.3389/fmed.2021.816802
_version_ 1784644349570580480
author Ke, Li
Chen, Luo
Yaling, Yuan
Can, Gao
Jun, Lin
Chuan, Zhang
author_facet Ke, Li
Chen, Luo
Yaling, Yuan
Can, Gao
Jun, Lin
Chuan, Zhang
author_sort Ke, Li
collection PubMed
description Chronic obstructive pulmonary disease (COPD) is a common obstructive respiratory disease characterized by persistent respiratory symptoms and limited airflow due to airway obstruction. The present study investigates the distribution characteristics of respiratory tract flora in both frequent and infrequent exacerbators of COPD. The 16S sequencing technique was adopted to differentiate the inherent differences of respiratory tract flora between frequent exacerbators and infrequent exacerbators. Additionally, cell counting kit 8 (CCK8), lactate dehydrogenase (LDH) test, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and western blot were carried out in human bronchial epithelial cells cultured in vitro and the regulatory effects of differential flora were verified. The results revealed that the observed species index, Chao1 index, and the ACE estimator of COPD frequent exacerbators were markedly higher than those of COPD infrequent exacerbators. The top five strains of COPD frequent exacerbators included g_Streptococcus (15.565%), g_Prevotella (10.683%), g_Veillonella (6.980%), g_Haemophilus (5.601%), and g_Neisseria (4.631%). Veillonella parvula generated obvious cytotoxicity and substantially reduced the activity of human bronchial epithelial cells (p < 0.01). Furthermore, the results of flow cytometry indicated that the proportion of human bronchial epithelial cells in both the S phase and G2 phase decreased following Veillonella parvula treatment indicated that Veillonella parvula inhibited cell proliferation. Meanwhile, being treated using Veillonella parvula, the expressions of interleukin-1 (IL-1), IL-6, Tumor Necrosis Factor α (TNF-α), and p-nuclear factor kappa B (NF-κB) of the cells were increased markedly (p < 0.01). Taken together, the current research demonstrated that the relative abundance of Veillonella in COPD frequent exacerbators was higher than that of infrequent exacerbators. Veillonella parvula activated the inflammatory pathway, ultimately destroyed the cell viability, and greatly impaired the activity of human bronchial epithelial cells, thereby inhibiting cell proliferation.
format Online
Article
Text
id pubmed-8811034
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88110342022-02-04 Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora Ke, Li Chen, Luo Yaling, Yuan Can, Gao Jun, Lin Chuan, Zhang Front Med (Lausanne) Medicine Chronic obstructive pulmonary disease (COPD) is a common obstructive respiratory disease characterized by persistent respiratory symptoms and limited airflow due to airway obstruction. The present study investigates the distribution characteristics of respiratory tract flora in both frequent and infrequent exacerbators of COPD. The 16S sequencing technique was adopted to differentiate the inherent differences of respiratory tract flora between frequent exacerbators and infrequent exacerbators. Additionally, cell counting kit 8 (CCK8), lactate dehydrogenase (LDH) test, flow cytometry, enzyme-linked immunosorbent assay (ELISA), and western blot were carried out in human bronchial epithelial cells cultured in vitro and the regulatory effects of differential flora were verified. The results revealed that the observed species index, Chao1 index, and the ACE estimator of COPD frequent exacerbators were markedly higher than those of COPD infrequent exacerbators. The top five strains of COPD frequent exacerbators included g_Streptococcus (15.565%), g_Prevotella (10.683%), g_Veillonella (6.980%), g_Haemophilus (5.601%), and g_Neisseria (4.631%). Veillonella parvula generated obvious cytotoxicity and substantially reduced the activity of human bronchial epithelial cells (p < 0.01). Furthermore, the results of flow cytometry indicated that the proportion of human bronchial epithelial cells in both the S phase and G2 phase decreased following Veillonella parvula treatment indicated that Veillonella parvula inhibited cell proliferation. Meanwhile, being treated using Veillonella parvula, the expressions of interleukin-1 (IL-1), IL-6, Tumor Necrosis Factor α (TNF-α), and p-nuclear factor kappa B (NF-κB) of the cells were increased markedly (p < 0.01). Taken together, the current research demonstrated that the relative abundance of Veillonella in COPD frequent exacerbators was higher than that of infrequent exacerbators. Veillonella parvula activated the inflammatory pathway, ultimately destroyed the cell viability, and greatly impaired the activity of human bronchial epithelial cells, thereby inhibiting cell proliferation. Frontiers Media S.A. 2022-01-20 /pmc/articles/PMC8811034/ /pubmed/35127772 http://dx.doi.org/10.3389/fmed.2021.816802 Text en Copyright © 2022 Ke, Chen, Yaling, Can, Jun and Chuan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Ke, Li
Chen, Luo
Yaling, Yuan
Can, Gao
Jun, Lin
Chuan, Zhang
Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora
title Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora
title_full Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora
title_fullStr Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora
title_full_unstemmed Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora
title_short Investigation on the Pathological Mechanism of Frequent Exacerbators With Chronic Obstructive Pulmonary Disease Based on the Characteristics of Respiratory Flora
title_sort investigation on the pathological mechanism of frequent exacerbators with chronic obstructive pulmonary disease based on the characteristics of respiratory flora
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811034/
https://www.ncbi.nlm.nih.gov/pubmed/35127772
http://dx.doi.org/10.3389/fmed.2021.816802
work_keys_str_mv AT keli investigationonthepathologicalmechanismoffrequentexacerbatorswithchronicobstructivepulmonarydiseasebasedonthecharacteristicsofrespiratoryflora
AT chenluo investigationonthepathologicalmechanismoffrequentexacerbatorswithchronicobstructivepulmonarydiseasebasedonthecharacteristicsofrespiratoryflora
AT yalingyuan investigationonthepathologicalmechanismoffrequentexacerbatorswithchronicobstructivepulmonarydiseasebasedonthecharacteristicsofrespiratoryflora
AT cangao investigationonthepathologicalmechanismoffrequentexacerbatorswithchronicobstructivepulmonarydiseasebasedonthecharacteristicsofrespiratoryflora
AT junlin investigationonthepathologicalmechanismoffrequentexacerbatorswithchronicobstructivepulmonarydiseasebasedonthecharacteristicsofrespiratoryflora
AT chuanzhang investigationonthepathologicalmechanismoffrequentexacerbatorswithchronicobstructivepulmonarydiseasebasedonthecharacteristicsofrespiratoryflora