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Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition

Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities an...

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Autores principales: Campbell, Tayte P., Ulrich, Danielle E. M., Toyoda, Jason, Thompson, Jaron, Munsky, Brian, Albright, Michaeline B. N., Bailey, Vanessa L., Tfaily, Malak M., Dunbar, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811196/
https://www.ncbi.nlm.nih.gov/pubmed/35126334
http://dx.doi.org/10.3389/fmicb.2021.799014
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author Campbell, Tayte P.
Ulrich, Danielle E. M.
Toyoda, Jason
Thompson, Jaron
Munsky, Brian
Albright, Michaeline B. N.
Bailey, Vanessa L.
Tfaily, Malak M.
Dunbar, John
author_facet Campbell, Tayte P.
Ulrich, Danielle E. M.
Toyoda, Jason
Thompson, Jaron
Munsky, Brian
Albright, Michaeline B. N.
Bailey, Vanessa L.
Tfaily, Malak M.
Dunbar, John
author_sort Campbell, Tayte P.
collection PubMed
description Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities and diverse substrate chemistries that occur in natural soils make it difficult to identify links between community membership and decomposition processes in the soil environment. To identify potential relationships between microbes, soil organic matter, and their impact on carbon storage, we used sand microcosms to control for external environmental factors such as changes in temperature and moisture as well as the variability in available carbon that exist in soil cores. Using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) on microcosm samples from early phase litter decomposition, we found that protein- and tannin-like compounds exhibited the strongest correlation to dissolved organic carbon (DOC) concentration. Proteins correlated positively with DOC concentration, while tannins correlated negatively with DOC. Through random forest, neural network, and indicator species analyses, we identified 42 bacterial and 9 fungal taxa associated with DOC concentration. The majority of bacterial taxa (26 out of 42 taxa) belonged to the phylum Proteobacteria while all fungal taxa belonged to the phylum Ascomycota. Additionally, we identified significant connections between microorganisms and protein-like compounds and found that most taxa (12/14) correlated negatively with proteins indicating that microbial consumption of proteins is likely a significant driver of DOC concentration. This research links DOC concentration with microbial production and/or decomposition of specific metabolites to improve our understanding of microbial metabolism and carbon persistence.
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spelling pubmed-88111962022-02-04 Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition Campbell, Tayte P. Ulrich, Danielle E. M. Toyoda, Jason Thompson, Jaron Munsky, Brian Albright, Michaeline B. N. Bailey, Vanessa L. Tfaily, Malak M. Dunbar, John Front Microbiol Microbiology Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities and diverse substrate chemistries that occur in natural soils make it difficult to identify links between community membership and decomposition processes in the soil environment. To identify potential relationships between microbes, soil organic matter, and their impact on carbon storage, we used sand microcosms to control for external environmental factors such as changes in temperature and moisture as well as the variability in available carbon that exist in soil cores. Using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) on microcosm samples from early phase litter decomposition, we found that protein- and tannin-like compounds exhibited the strongest correlation to dissolved organic carbon (DOC) concentration. Proteins correlated positively with DOC concentration, while tannins correlated negatively with DOC. Through random forest, neural network, and indicator species analyses, we identified 42 bacterial and 9 fungal taxa associated with DOC concentration. The majority of bacterial taxa (26 out of 42 taxa) belonged to the phylum Proteobacteria while all fungal taxa belonged to the phylum Ascomycota. Additionally, we identified significant connections between microorganisms and protein-like compounds and found that most taxa (12/14) correlated negatively with proteins indicating that microbial consumption of proteins is likely a significant driver of DOC concentration. This research links DOC concentration with microbial production and/or decomposition of specific metabolites to improve our understanding of microbial metabolism and carbon persistence. Frontiers Media S.A. 2022-01-20 /pmc/articles/PMC8811196/ /pubmed/35126334 http://dx.doi.org/10.3389/fmicb.2021.799014 Text en Copyright © 2022 Campbell, Ulrich, Toyoda, Thompson, Munsky, Albright, Bailey, Tfaily and Dunbar. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Campbell, Tayte P.
Ulrich, Danielle E. M.
Toyoda, Jason
Thompson, Jaron
Munsky, Brian
Albright, Michaeline B. N.
Bailey, Vanessa L.
Tfaily, Malak M.
Dunbar, John
Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
title Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
title_full Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
title_fullStr Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
title_full_unstemmed Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
title_short Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
title_sort microbial communities influence soil dissolved organic carbon concentration by altering metabolite composition
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8811196/
https://www.ncbi.nlm.nih.gov/pubmed/35126334
http://dx.doi.org/10.3389/fmicb.2021.799014
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